|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 健康成人24h尿液细胞外囊泡定量及大小分布显示文摘目的研究观察健康成人24 h尿液细胞外囊泡(u EVs)数量、大小分布及个体间差异。方法利用液压透析滤过法分离9名健康成年人24 h u EVs,通过western blot(WB)和透射电镜(TEM)技术验证u EVs的分离效果,应用BCA蛋白定量法及纳米微粒跟踪分析技术分别对富集的u EVs样品进行蛋白定量测定和囊泡数量及大小分布的分析。结果 TEM下可见大小不等、形状不一的囊泡。WB技术检测到外泌体的标记物—人肿瘤易感基因(TSG101)。9名健康成人24 h尿液分离的u EVs样品蛋白定量范围为132.50~760.70 ng/m L。纳米微粒跟踪分析技术检测结果显示24 h u EVs数量为(3.56~5.12)×1012个,CV=14.23%。24 h分泌的u EVs中,直径在〈40 nm范围内的囊泡占所检测到的总囊泡数的0.04~0.69%,数量为(1.80~26.49)×109个;符合外泌体直径范围(40~100 nm)的囊泡占总囊泡数的22.07%~42.08%,数量为(1.00~1.77)×1012个。符合微囊泡直径范围(100~1000 nm)的囊泡约占总囊泡数的57.88%~77.85%,数量为(2.09~3.86)×1012个。结论新型液压透析滤过法能高效、便捷地实现大样本尿液u EVs的分离。健康成人分泌的24 h u EVs个体间差异较小,是u EVs相关研究较理想的标本来源。 | 林韩翡 刘新宇 徐小蒙 Luca Musante Harry Holthofer 邹和群 | 2015 | 南方医科大学学报2015，35，11： | 3 |
| 2 | Proportional assist ventilation in low birth weight infants with acute respiratory disease: A comparison to assist/control and conventional mechanical ventilation显示文摘 | Schulze A Gerhardt T Musante G | 1999 | Pediatry1999，135，3： | 1 |
| 3 | Blue silver:A very sensitive eoUoidal Coomassie G - 250 staining for proteome analysis 显示文摘 | Candiano G Brusehi M Musante L | 2004 | Eleetrophoresis2004，25，9： | 1 |
| 4 | Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis 显示文摘 | Candiano G Bruschi M Musante L | 2004 | Electrophoresis2004，25，9： | 1 |
| 5 | Magnetic resonance imaging in local staging of endometrial carcinoma:Diagnostic performance,pitfalls and literature review显示文摘 | Zandrino F La Paglia E Musante F | 2010 | Tumori2010，96，4： | 1 |
| 6 | Blue silver:a very sensitive colloidal Coomassie G‐250 staining for proteome analysis显示文摘 | Candiano G Bruschi M Musante L | 2004 | Electrophoresis2004，25，9： | 1 |
| 7 | MR imaging of adrenalmyelo lipomas显示文摘 | Musante F Derchi LE Bazzoch IM | 1991 | JCAT1991，15，1： | 1 |
| 8 | Blue silver: a very sensi- tive colloidal Coomassie G-250 staining for proteome analysis 显示文摘 | Candiano G Bruschi M Musante L | 2004 | Electrophoresis2004，25，9： | 1 |
| 9 | Blue silver:a very sensitive colloidal Coomassie G-250 staining for proteome analysis显示文摘 | Candiano G Bruschi M Musante L | 2004 | Electrophoresis2004，25，： | 1 |
| 10 | Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis 显示文摘 | CANDIANO G BRUSCHI M MUSANTE L | 2004 | Electrophoresis2004，25，9： | 1 |
| 11 | Pre-synaptic glycine GlyT1 transporter-NMDA receptor interaction: relevance to NMDA autoreceptor activation in the presence of Mg^2+ ions显示文摘 | Musante V Summa M Cunha RA | 2011 | J Neurochem2011，117，3： | 1 |
| 12 | Blue silver:A very sensitive colloidal Coomassie G-250 staining for proteome analysis显示文摘 | Candiano G Bruschi M Musante L | | 0，，09： | 1 |
| 13 | Characterization of plasma factors that alter the permeability to albumin within isolated glomeruli 显示文摘 | Musante L Candiano G Bruschi M | 2002 | Proteomics2002，2，2： | 1 |
| 14 | cDNA cloning and characterization of the human THRAP2 gene which maps to chromosome 12q24,and its mouse ortholog Thrap2显示文摘 | Musante L Bartsch O Ropers HH | 2004 | Gene2004，332，： | 1 |
| 15 | Con- current plant uptake of heavy metals and persistent organic pollutants form soil 显示文摘 | Mattina M J I Lannucci Berger W Musante C | 2003 | Environmental Pollution2003，124，3： | 1 |
| 16 | Magnetic resonauce imaging in local staging of endometrial carcinoma:Diagnostic performance,pitfalls,and literature review显示文摘 | Zandrino F La Paglia E Musante F | 2010 | Tumori2010，96，4： | 1 |
| 17 | Blue silver: a very sensitive colloidal coomassie G-250 staining for proteome analysis 显示文摘 | Candiano G Bruschi M Musante L | 2004 | Electrophoresis2004，25，9： | 1 |
| 18 | Blue silver: A very sensitive colloidal Coomassie G--250 staining for proteome analysis显示文摘 | Giovanni Candiano Maurizio Bruschi Luca Musante Laura Santueci Gian Marco Ghiggeri Barbara Carnemolla Paola Orecchia Luciano Zardi Pier Giorgio Righetti | 2004 | Electrophoresis2004，25，： | 1 |
| 19 | Characterization of plasma factors that alter the permeability to albumin within isolated glomeruli显示文摘 | | 2002 | Proteomics2002，2，： | 1 |
| 20 | Effect of volume-guaranteed synchronized intermittent mandatory ventilation in preterm infant recovering from respiratory failure 显示文摘 | Herrera CM Gerhardt T Claure N Everett R Musante G Thomas C | 2002 | Pediatrics2002，110，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员