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| 1 | Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low-and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs显示文摘Background: Bacillus spp. seem to be an alternative to antimicrobial growth promoters for improving animals' health and performance. However, there is little information on the effect of Bacillus spp. in combination with different dietary crude protein(CP) levels on the ileal digestibility and microbiota composition. Therefore, the objective of this study was to determine the effect of Bacillus spp. supplementation to low-(LP) and high-protein diets(HP) on ileal CP and amino acid(AA) digestibility and intestinal microbiota composition.Methods: Eight ileally cannulated pigs with an initial body weight of 28.5 kg were randomly allocated to a rowcolumn design with 8 pigs and 3 periods of 16 d each. The assay diets were based on wheat-barley-soybean meal with two protein levels: LP(14% CP, as-fed) and HP diet(18% CP, as-fed). The LP and HP diets were supplemented with or without Bacil us spp. at a level of 0.04%(as-fed). The apparent ileal digestibility(AID) and standardized ileal digestibility(SID) of CP and AA was determined. Bacterial community composition from ileal digesta was analyzed by Illumina amplicon sequencing and quantitative real-time PCR. Data were analyzed as a 2 × 2 factorial design using the GLIMMIX procedures of SAS.Results: The supplementation with Bacillus spp. did not affect both AID and SID of CP and AA in growing pigs.Moreover, there was no difference in AID of CP and AA between HP and LP diets, but SID of cystine, glutamic acid,glycine, and proline was lower(P < 0.05) in pigs fed the HP diets. The HP diets increased abundance of Bifidobacterium spp. and Lactobacillus spp.,(P < 0.05) and by amplicon sequencing the latter was identified as predominant genus in microbiota from HP with Bacillus spp., whereas dietary supplementation of Bacillus spp. increased(P < 0.05) abundance of Roseburia spp.Conclusions: The HP diet increased abundance of Lactobacillus spp. and Bifidobacterium spp.. The supplementation of Bacil us spp. resulted in a higher abundance of healthy gut associated bacteria without affecting ileal CP and AA digestibility, whereas LP diet may reduce the flow of undigested protein to the large intestine of pigs. | Chanwit Kaewtapee Katharina Burbach Georgina Tomforde Thomas Hartinger Amelia Camarinha-Silva Sonja Heinritz Jana Seifert Markus Wiltafsky Rainer Mosenthin Pia Rosenfelder-Kuon | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 5 |
| 2 | Determination of basal ileal endogenous losses and standardized ileal digestibility of amino acids in barley fed to growing pigs显示文摘Background: Basal ileal endogenous amino acid(AA) losses(IAA_(end)) and standardized ileal digestibility(SID) values of cereal grains, such as barley, are apparently underestimated when determined according to the nitrogen(N)-free method. Regression analysis between the dietary apparent ileal digestible content(c AID) and total crude protein(CP) and AA can be considered as alternative approach to obtain more accurate values for IAA_(end)and SID of AA in cereal grains.Methods: Eight hulled barley genotypes were used, with barley being the only source of CP and AA in the assay diets. The diets contained 95 % as-fed of these eight barley genotypes each, ranging in CP content between 109.1 and 123.8 g/kg dry matter(DM). Nine ileally T-cannulated barrows, average body weight(BW) 30 ± 2 kg, were allotted to a row-column design comprising eight periods with 6 d each and nine pigs. On d 5 and the night of d 6 of every period, ileal digesta were collected for a total of 12 h. The IAA_(end) and the SID were determined by linear regression analysis between c AID and total dietary CP and AA.Results: There exist linear relationships between cA ID and total CP and AA(P < 0.001). The IAA_(end) of CP, Lys, Met, Thr and Trp amounted to 35.34, 1.08, 0.25, 1.02 and 0.38 g/kg DM intake(DMI), respectively, which are greater compared to average IAA_(end) determined previously under N-free feeding conditions. The SID of CP, Lys, Met, Thr and Trp was 90,79, 85, 79 and 86 %, respectively, and was greater when compared to tabulated values. Moreover, these SID values were greater than those reported in literature, based on correction of apparent ileal digestibility(AID) of CP and AA for their IAA_(end)values. Summarized, the results of the present regression analysis indicate greater IAA_(end)in barley-based diets compared to those obtained by N-free feeding.Conclusions: For low-protein feed ingredients like barley the regression method may be preferred over correction of AID values for their IAA_(end)determined under N-free feeding conditions, as intercepts and slopes of the linear regression equations between cA ID and total dietary CP and AA provide direct estimates of IAA_(end)and SID of CP and AA in the presence of the assay feed ingredient. | Hanna Katharina Spindler Rainer Mosenthin Pia Rosenfelder Henry J?rgensen Knud Erik Bach Knudsen Meike Eklund | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 2 |
| 3 | Effect of supplementation of xylanase and phospholipase to a wheat-based diet for weanling pigs on nutrient digestibility and concentrations of microbial metabolites in ileal digesta and feces显示文摘 | DIEBOLD G MOSENTHIN R PIEPHO H P | 2004 | Jouranl of Animal Science2004，82，9： | 1 |
| 4 | Effect of fumaric acid and dietary buffering capacity on ileal and fecal amino digestibility in early - weaned pigs 显示文摘 | BLANK R MOSENTHIN R SAUER W C | 1999 | J Anita Sci1999，77，： | 1 |
| 5 | E- valuation of the SN-isotope dilution technique for de- termining the recovery of endogenous protein in ileal digestion of pigs: effect of dilution in the precursor pool for endogenous nitrogen secretion 显示文摘 | LIEN K A SAUER W C MOSENTHIN R | 1997 | Journal of Animal Science1997，75，1： | 1 |
| 6 | Effect of supplementation of xylanase and phospho-lipase to a wheat-based diet for weanling pigs on nutri-ent digestibility and concentrations of microbial metab-olites in ileal digesta and feces 显示文摘 | DIEBOLD G MOSENTHIN R PIEPHO H P | 2004 | Journal of AnimalScience2004，82，9： | 1 |
| 7 | Effect of fumaric acid and dietary buffering capacity on ileal and fecal amino acid digestibili- ties in early-weaned pigs显示文摘 | Blank R Mosenthin R Sauer W C | 1999 | Journal of Animal Science1999，77，： | 1 |
| 8 | Evaluation of the 15N-isotope dilution technique for determining the recovery of endogenous protein in ileal digestion of pigs:Effect of dilution in the precursor pool for endogenous nitrogen secretion显示文摘 | Lien K A Sauer W C Mosenthin R | 1997 | J Anim Sci1997，75，： | 1 |
| 9 | Effect of fumaric acid supplementation and dietary buffering capacity on the concentra- tion of microbial metabolites in digesta of young pigs显示文摘 | Blank R Sauer W C Mosenthin R | 2001 | Canadian Journal of Animal Science2001，81，： | 1 |
| 10 | The use of grain legumes as a protein source in pig nutrition: A review 显示文摘 | Jezierny D Mosenthin R Bauer E | 2010 | Anim Feed Sci Technol2010，157，3： | 1 |
| 11 | Metabolic, osmoregulatory and nutritional functions of betaine in monogastric animals 显示文摘 | Ratriyanto A Mosenthin R Bauer E | 2009 | Asian Austral J Anita Sci2009，22，： | 1 |
| 12 | Effect of supplementation of xylanase and phospholipase to a wheat-based diet for weanling pigs on nutrient digestibility and concentrations of microbial metabolites in ileal digesta and feces显示文摘 | DIEBOLD G MOSENTHIN R PIEPHO H P | 2004 | Journal of Animal Science2004，82，9： | 1 |
| 13 | Effects of betaine and condensed molasses solubles on ileal and t~tal tract nutrient digestibilities in piglets 显示文摘 | Eklund M Mosenthin R Piepho H P | 2006 | Acta Agric Scand Section A2006，，56： | 1 |
| 14 | Oat p-glucan and dietary calciumand phosphorus differentially modify intestinal expres-sion of proinflammatory cytokines and monocarboxylatetransporter 1 and cecal morphology in weaned pigs 显示文摘 | METZLER-ZEBELI B U GANZLE M G MOSENTHIN R Et al | 2012 | The Journal of Nutrition2012，142，4： | 1 |
| 15 | The effect of dietary phosphorus and calcium level,phytase supplementation,and ileal infusion of pectin on the chemical composition and carbohydrase activity of fecal bacteria and the level of microbial metabolites in the gastrointestinal tract of pigs显示文摘 | Metzler B Mosenthin R Baumgrtel T | 2008 | Journal of Animal Science2008，86，7： | 1 |
| 16 | Digestibility mesurements in pigs by using post-value T-caccrm cannulation or end-to-side ileo-netal anastomosis 显示文摘 | Koehler T Den Hartog L A Huisman J Mosenthin R | 1991 | J Anita Physiolog Anita Nutr1991，66，： | 1 |
| 17 | Principles of Physiology of Lipid Digestion Asian-Aust显示文摘 | Bauer E Jakob S Mosenthin R | 2005 | Anum Sci2005，18，2： | 1 |
| 18 | Effect of fumaric acid and dietary buffering capacity on ileal and fecal amino acid digestibilities in early-weaned pigs显示文摘 | Blank R Mosenthin R Sauer W C | 1999 | Journal of Animal Science1999，77，11： | 1 |
| 19 | The role of dietary nucle- otides in single-stomached animals显示文摘 | SAUER N MOSENTHIN R BAUER E | 2011 | Nutr Res Rev2011，24，： | 1 |
| 20 | Dietary pectin's effect on ileal and fecal amino acid digestibility and exocrine pancreatic secretinns in growing pigs显示文摘 | Mosenthin R Saucier W C Ahrens F | 1994 | J Nutr1994，124，： | 1 |
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