| 4 | Plasma effects on the bacteria Escherichia coli via two evaluation methods显示文摘The degradation of?Escherichia coli?bacteria?by treatment with?cold,?weakly ionised, highly dissociated oxygen plasma,?with?an?electron temperature of 3 e V, a?plasma density of 8?×?10^(15)m^(-3) and a?neutral oxygen atom density of 3.5?×?1021m^(-3) was studied. To determine the ‘real' plasma effects,?two methods were used for evaluation and determination, as well as a comparison of the number of?bacteria that had?survived: the standard plate count technique(PCT) and?advanced fluorescence-activated cell sorting(FACS). Bacteria were deposited onto glass substrates and kept below 50 °C during the experiments with oxygen plasma. The results showed that the?bacteria had?fully degraded after about 2 min of plasma treatment, depending slightly on the amount of bacteria that had been?deposited on the substrates. The very?precise determination of the O flux on?the substrates and the two-method comparison allowed for the determination of the critical dose of oxygen atoms required for the destruction of a bacterial cell wall—about 6?×?10^(24)m^(-2)—as well as deactivation of the substrates—about 8?×?1025m^(-2).These results were taken in order to discuss other results obtained by comparable studies and scientific method?evaluations in the determination of plasma effects on bacteria. | Danijela VUJO?EVI? Uro? CVELBAR Ur?ka REPNIK Martina MODIC Sa?a LAZOVI? Tina ZAVA?NIK-BERGANT Nevena PUA? Boban MUGO?A Evangelos GOGOLIDES Zoran Lj PETROVI? Miran MOZETI? | 2017 | Plasma Science and Technology2017，19，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员