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| 1 | Genes transactivated by hepatitis C virus core protein, a microarray assay显示文摘AIM: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.METHODS: Reverse transcribed cDNA was subjected tomicroarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.RESULTS: The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene namedHCTP4 was cloned with molecular biological method in combination with bioinformatics method.CONCLUSION: HCV core is a potential transactivator.Microarray is an efficient and convenient method for analysis of differentially expressed genes. | MinLiu Shu-LinZhang JunCheng YanLiu LinWang QingShao JianZhang Shu-MeiLin | 2005 | World Journal of Gastroenterology2005，11，22： | 5 |
| 2 | Transactivating effect of hepatitis C virus core protein: A suppression subtractive hybridization study显示文摘AIM: To investigate the transactivating effect of hepatitis C virus (HCV) core protein and to screen genes transactivated by HCV core protein. METHODS: pcDNA3.1(-)-core containing full-length HCV core gene was constructed by insertion of HCV core gene into EcoRI/BarnHI site. HepG2 cells were cotransfected with pcDNA3.1(-)-core and pSV-lacZ. After 48 h, cells were collected and detected for the expression of β-gal by an enzyme-linked immunosorbent assay (ELISA) kit. HepG2 cell swere transiently transfected with pcDNA3.1(-)-core using Upofectamine reagent. Cells were collected and total mRNA was isolated. A subtracted cDNA library was generated and constructed into a pGEM-Teasy vector. The library was amplified with E. coil strain JM109. The cDNAs were sequenced and analyzed in GenBank with BLAST search after polymerase chain reaction (PCR). RESULTS: The core mRNA and protein could be detected in HepG2 cell lysate which was transfected by the pcDNA3.1(-)-core. The activity of β-galactosidase in HepG2 cells transfected by the pcDNA3.1(-)-core was 5.4 times higher than that of HepG2 cells transfected by control plasmid. The subtractive library of genes transactivated by HCV core protein was constructed successfully. The amplified library contained 233 positive clones. Colony PCR showed that 213 clones contained 100-1 000 bp inserts. Sequence analysis was performed in 63 clones. Six of the sequences were unknown genes. The full length sequences were obtained with bioinformatics method, accepted by Genl3ank. It was suggested that six novel cDNA sequences might be target genes transactivated by HCV core protein. CONCLUSION: The core protein of HCV has transactivating effects on SV40 early promoter/enhancer. A total of 63 clones from cDNA library were randomly chosen and sequenced. Using the BLAST program at the National Center for Biotechnology Information, six of the sequences were unknown genes. The other 57 sequences were highly similar to known genes. | MinLiu YanLiu ]unCheng Shu-LinZhang LinWang QingShao JianZhang QianYang | 2004 | World Journal of Gastroenterology2004，10，12： | 4 |
| 3 | Assessment of Impacts of Public Health Interventions on the SARS Epidemic in Beijing in Terms of the Intervals Between Its Symptom Onset, Hospital Admission, and Notification显示文摘Objectives To assess the impacts of public health interventions on the outbreak of SARS in Beijing by analyzing the intervals between symptom onset, hospital admission and notification of its cases. Methods Data of SARS cases reported from the Beijing Municipal Centers for Disease Prevention and Control (BCDC) were collected and analyzed by descriptive epidemiology. Results In the early epidemic period, the intervals between the disease onset and the hospital admission seemed irregular, so was the intervals between the hospital admission and the notification. After the middle ten days of April, the intervals turned out to be more regular, and the disordered situation in terms of the hospital admission and the case notification was gradually brought under control. Conclusions Public health interventions against SARS has revealed positive impacts on SARS control program in Beijing. The timing and sensitivity of epidemic information reporting systems has been greatly improved in Beijing as a result of successful fight against this disease. | WAN-NIANLIANG MINLIU ZE-JUNLIU XIONGHE YUEPAN XUE-QINXIE | 2005 | Biomedical and Environmental Sciences2005，18，3： | 3 |
| 4 | Production of human liver prolidase by Saccharomyces cerevisiae as host cells显示文摘AIM: To clone and express the recombinant human liver prolidase in yeast and explore the activities of both dipeptidaseand organophosphoric acid anhydrolase(OPAA). METHODS:The cDNAencodinghuman liver prolidase derived from healthy adult liver was cloned into the pYES2, an expression vector of S cerevisiae, and then trans- formed into S cerevisiae INVSc1 by electroporation. The transformant with the highest enzymatic activity was induced by galactose for expression. The optimal induction conditions (temperature, induction time, and the initial amount of inoculation cells) were estimated by orthogonal experimental design. The recombinant prolidase and OPAA activities were assayed by spectrocolorimetric methods. RESULTS: The recombinant enzyme catalyzed the hydrolysis of organophosphorous compound soman as well as the hydrolysis of dipeptide Gly-Pro. Under the optimal induction conditions (20 h, 25 oC, initial OD600=0.4), the maximum activities of prolidase and OPAA came to 226.5 and 578 μmol·min-1·g-1 protein in cell lysate supernatants, respectively. SDS-PAGE of the recombinant enzyme in disrupted cell supernatants showed a molecular weight of 56 kDa. Intensity scanning of the SDS-PAGE gel revealed that the enzyme accounted for 3.16 % of the total protein in the supernatant. One liter incubation medium produced 7 g of wet yeast cell containing 4.56 mg of the recombination protein. CONCLUSION: The recombinant human liver prolidase produced by yeast cell (S cerevisiae) exhibited both dipeptidase and OPAA activities. | Shu-haoWANG MinLIU Mu-genCHI Qing-dingWANG Man-jiSUN | 2004 | Acta Pharmacologica Sinica2004，25，6： | 2 |
| 5 | Growth arrest and apoptosis of human hepatocellular carcinoma cells induced by hexamethylene bisacetamide显示文摘AIM: To investigate the cellular effects of hybrid polar compound hexamethylene bisacetamide (HMBA) on the growth and apoptosis of human hepatocellular carcinoma cells and to provide the molecular mechanism for potential application of HMBA in the treatment of liver cancer. METHODS: Effects of HMBA on the growth of human hepatocellular carcinoma SMMC-7721 cells were assayed by MTT chronometry. Apoptosis induced by HMBA was detected by phase-contrast microscopy, flow cytometry, propidium iodide staining and immunocytochemical analysis. RESULTS: The growth of SMMC-7721 cells was significantly inhibited by HMBA, and the growth inhibitory rate was 51.1%, 62.6%, 68.7% and 73.9% respectively after treatment with 5.0, 7.5, 10.0 and 12.5 mmol/L of HMBA. In the cells treated with 10 mmol/L of HMBA for 72 h, the population of cells at sub-G1 phase significantly increased, and the apoptotic bodies and condensed nuclei were detected. Moreover, treatment of SMMC-7721 cells with 10 mmol/L of HMBA down-regulated the expression of Bcl2 anti-apoptotic protein, while slightly up-regulated the level of pro-apoptotic protein Bax. CONCLUSION: Treatment with 10.0 mmol/L of HMBA can significantly inhibit the growth and induce apoptosis of human hepatocellular carcinoma SMMC-7721 cells by decreasing the ratio of Bcl-2 to Bax. | Gao-LiangOuyang Qiu-FengCai MinLiu Rui-ChuanChen ZhiHuang Rui-ShengJiang FuChen Shui-GenHong Shi-DengBao | 2004 | World Journal of Gastroenterology2004，10，7： | 2 |
| 6 | MicroRNA‐9 inhibits ovarian cancer cell growth through regulation of NF‐κB1显示文摘 | TaoLiu Yi‐XuanLi MinLiu XinLi HuaTang | 2009 | FEBS Journal2009，，19： | 2 |
| 7 | Effects of land-use categorization on landscape metrics:a case study in urban landscape of Shenzhen,China显示文摘 | PENG Jing WANG Yangyuan YE Minliu | | 0，，21： | 1 |
| 8 | Enhanced Secretion of Heterologous Proteins in Pichia pastoris Following Overexpression of Saccharomyces cerevisiae Chaperone Proteins显示文摘 | WeiZhang Hong‐liangZhao ChongXue Xiang‐huaXiong Xue‐qinYao Xian‐yuLi Hui‐pengChen Zhi‐minLiu | 2008 | Biotechnol Progress2008，，4： | 1 |
| 9 | MicroRNA‐23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin‐6 receptor显示文摘 | Li‐HuaZhu TaoLiu HuaTang Rui‐QingTian ChangSu MinLiu XinLi | 2010 | FEBS Journal2010，，18： | 1 |
| 10 | Synthesis of yt- trium aluminum garnet (YAG) powder by homogeneous pre- cipitation combined with supercritical carbon dioxide or ethanol fluid drying显示文摘 | Yang Ru Qin Jie Li MinLiu Guoqiang | 2008 | Journal of the Euro- pean Ceramic Society2008，28，： | 1 |
| 11 | Effect of sand burial depth on seed germination and seedling emergence of Calligonum L species显示文摘 | Jun Ren Ling Tao Xin Minliu | 2002 | Journal of Arid Environments2002，51，： | 1 |
| 12 | mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C. elegans显示文摘MicroRNA (miRNA ) 在许多细胞的事件调整基因表示,然而,仅仅一些 miRNAs 的功能在 C 被知道。elegans。我们分析了对 mir-35 调整的这里的蠕虫,和表演唯一的 mir-35-41 的功能肠的房间和细菌房间增长的 G1/S 转变。mir-35 的损失在肠和远侧的有丝分裂的性腺导致原子核数字的减少,当 mir-35 的再介绍救变异的显型时。基因分析显示 mir-35 可以通过 Rb/E2F 和 SCF 小径行动。进一步生物信息、功能的分析证明 mir-35 目标进化地保存了 lin-23 和 gld-1。一起,我们的学习在房间分割揭示 mir-35 家庭的新奇功能规定。 | MinLiu PengpengLiu LiZhang QingchunCai GeGao WenxiaZhang ZuoyanZhu DongLiu QichangFan | 2011 | Cell Research2011，21，11： | 1 |
| 13 | Specific Features of the Contact History of Probable Cases of Severe Acute Respiratory Syndrome显示文摘Objective To describe the specific features of the contact history of probable cases of severe acute respiratory syndrome (SARS) in Beijing. Methods Data of SARS cases notified from the Beijing Municipal Center for Disease Control and Prevention (BCDC) and supplemented by other channels were collected. All the data were analyzed by descriptive epidemiology. Results ①The number of probable cases with contact history was significantly higher than the excluded cases. ②The proportion of probable cases with contact history descended with epidemic development, but this situation did not occur in health care workers (HCWs). ③The fatality rate of probable cases with contact history was significantly higher than the cases without contact history (OR=1.489). ④The proportion of probable cases with contact history was 85.86% among health care workers, which was significantly higher than that of non-health care workers (85.86% v.s. 56.44%, OR=4.69). Conclusions ①The susceptible persons with contact history may not get infected, and the contact history is just a sufficient condition of infecting SARS; ②There are 3 conceivable reasons for the descending trend of the proportion in probable cases with contact history; ③The contact history is one of the risk factors of the death of SARS cases; ④The risk of contacting with SARS among health care workers is approximately 5 times higher than that of non-HCWs. | WAN-NIANLIANG MINLIU QICHEN ZE-JUNLIU XIONGHE XUE-QINXIE | 2005 | Biomedical and Environmental Sciences2005，18，2： | 0 |
| 14 | Synthesize of 2,6-Di-O-acetyl Sucrose by Regioselective Acetylation with Dibutyltin Oxide显示文摘Di-O-acetyl sucrose was regioselective synthesized by using dibutyltin oxide in about 60% yield. The structure of acetyl derivative was elucidated by 1H NMR, 13C NMR, 2D-NMR and MS spectral data. | YuKE MinLIU ZhenZhongLIU | 2004 | Chinese Chemical Letters2004，15，3： | 0 |
| 15 | Analysis on thoracic stab of 210 patients in clinical anatomy显示文摘 | JunHu RuiWang MingzhiChen BinMeng WeiTao JianCao PengXu MinLiu ZhengweiWang DishengMeng JunyuXu | 2004 | 中国组织化学与细胞化学杂志2004，13，3： | 0 |
| 16 | HMLH1 gene mutation in gastric cancer patients and their kindred显示文摘AIM: To study the status of hMLH1 gene point mutations of gastric cancer kindreds and gastric cancer patients from northern China, and to find out gene mutation status in the population susceptible to gastric cancer. METHODS: Blood samples of 120 members from five gastric cancer families, 56 sporadic gastric cancer patients and control individuals were collected. After DNA extraction,the mutations of exon 8 and exon 12 of hMLH1 gene were investigated by PCR-SSCP-CE, followed by DNA sequencing.RESULTS: In the five kindreds, the mutation frequency was 25% (5/16) for the probands and 18% (19/104) for the non-cancerous members, which were significantly higher than the controls (P<0.01 x2 = 7.71, P<0.01 x2 = 8.65, respectively). In the sporadic gastric cancer, the mutation frequency was 7% (4/56), which was similar to that (5/100) in the healthy controls. The mutation point of exon 8 was at 219 codon of hMLH1 gene (A-G), resulting in a substitution of Ile-Val (ATC-GTC), whereas the mutation of exon 12 was at 384 codon of hMLH1 gene (T-A) resulting in a substitution of Asp-Val (GTT-GAT), which were the same as previously found in hereditary nonpolyposis colorectal carcinoma.CONCLUSION: The members of gastric cancer families from northern China may have similar genetic background of hMLH1 gene mutation as those of hereditary nonpolyposis colorectal carcinoma. | Jian-HuaLi Xian-ZheShi ShenLü MinLiu Wan-MingCui Li-NaLiu JingJiang Guo-WangXu | 2005 | World Journal of Gastroenterology2005，11，20： | 0 |
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