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| 1 | Hydrogen sutfide protects colon cancer cells from chemopreventative agent β-phenylethyl isothiocvanate induced apoptosis显示文摘AIM: Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent β-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells.METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubG1 population analysis,caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage.RESULTS: PEITC significantly induced apoptosis in HGT116cells as assessed by SubG1 population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described.CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the response of colonic epithelial cells to beneficial as well as toxic agents present within the GI tract. | Peter Rose Philip K Moore Shen Han Ming Ong Choon Nam Jeffrey S Armstrong Matt Whiteman | 2005 | World Journal of Gastroenterology2005，11，26： | 13 |
| 2 | 无压力室喷油嘴液力研磨对直喷式柴油机混合气形成的影响显示文摘液力研磨(HG)工艺可应用于无压力室喷油嘴(VCO)产品上。当确定VCO喷油嘴液力研磨对增压型高速直喷式柴油机混合气形成的影响,进行了全面的理论计算与试验研究。搞清了液力研磨喷油嘴的喷束特性能满足增压型高速直喷式(HSDI)柴油机混合气形成的要求。 | Kampmann S Dittus B Mattes P 何庆康 | 1998 | 国外内燃机1998，30，4： | 2 |
| 3 | The Performance of Remote Display Mechanisms for Thin-Client Computing显示文摘 | S Jae Yong Jason Nieh Matt Selsky | 2002 | In:Proceddings of the 2002 USENIX Annual Technical Conference2002，，6： | 1 |
| 4 | HCN2 channels in localinhibitory interneurons constrain LTP in the hippocampal direct per-forant path显示文摘 | Matt L Michalakis S Hofmann F | 2011 | Cell Mol Life Sci2011，68，： | 1 |
| 5 | Atrial fibrillation and acute myocardial infarction without significant coronary stenoses associated with subclinical hyperthyroidism and erythrocytosis显示文摘 | Patan6 S Matte F | 2010 | International Journal of Cardiology2010，145，1： | 1 |
| 6 | Involvement of fibrocytes in asthma and clinical implications显示文摘 | Matt oli S | 2015 | Clin Exp Allergy2015，45，10： | 1 |
| 7 | Influence of high fiber diets given to gestating sows on serum concentrations of micronutrients显示文摘 | Girard C L Robert S Matte J J | 1995 | Livestock Producion Science1995，43，： | 1 |
| 8 | Sensitization of low bandgap polymer bulk heterojunction solar cells显示文摘 | WINDER C MATT G HUMMELEN C JAMSSEN R A J SARICIFTCI N S BRABEC C J | 2002 | Thin Solid Films2002，404，： | 1 |
| 9 | Exon 11 mutations, Ki67, and p16(INKgA) as predictors of prognosis in patients with GIST 显示文摘 | Cerski M R Pereira F Matte U S | 2011 | Pathol Res Pract2011，207，11： | 1 |
| 10 | Coping style use predicts posttraumatic stress and complicated grief symptom severity among college students reporting a traumatic loss显示文摘 | Kimberly R S Jon D E Matt J G | 2007 | Journal of Counseling Psychology2007，54，3： | 1 |
| 11 | NO in exhaled air is correlated with markers of eosinophilic airway inflammation in corticosteroid-dependent childhood asthma 显示文摘 | Mattes J Storm van's Gravesande K | 1999 | Eur Respir J1999，13，： | 1 |
| 12 | Sustained Benefit From a Long-Term Antiretroviral Adherence Intervention显示文摘 | Mannheimer S Morse E Matts J | 2006 | JAIDS2006，43，1： | 1 |
| 13 | Regulation of microRNA by an tagomirs:a new class of pharmacological antagonists for the specific regulation of gene function显示文摘 | Mattes J Yang M Foster P S | 2007 | American Journal of Respiratory Cell and Molecular Biology2007，36，1： | 1 |
| 14 | Laser brazing of a steel/aluminium assembly with hot filler wire (88%Al,12%Si)显示文摘 | MATHIEU A PONTEVICCI S VIALA J C CICALA E MATTE S GREVEY D | 2006 | Mater Sci Eng A2006，，5： | 1 |
| 15 | Hydroxy fatty acids as indicators for ageing and the influence of oxygen in the brewhouse on the flavour stability of beer显示文摘 | Wackerbauer K Meyna S Matte S | 2003 | Monatsschrift Fur Brauwissenschaft2003，56，910： | 1 |
| 16 | lnfluence of high fiber diets given to gestating sows on serum concentrations of micronutri- ents显示文摘 | Girard C L Robert S Matte J J et d | 1995 | Livestock Producion Science1995，43，： | 1 |
| 17 | Aerobic stability and in vitro fiber digestibility of microbially inoculated corn and sorghum silages显示文摘 | Matt A S | 1993 | Journal of Animal Science1993，71，2： | 1 |
| 18 | Gra- phene analogues of inorganic layered materials显示文摘 | RAO CNR MATTE H S S R MAITRA U | 2013 | Angew Chem2013，52，13： | 1 |
| 19 | Biomass production of 'Alamo' switchgrass in response to nitrogen,phosphorous and row spacing显示文摘 | James P M Matt A S William R O | 2001 | Agronomy Journal2001，93，4： | 1 |
| 20 | Successful RNA extraction from various human postmortem tissues显示文摘 | Heinrich M Matt K Lutz-Bonengel S | 2007 | Int J Leg Med2007，121，2： | 1 |
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