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2篇 您的检索式:作者名="Mathieu Erhardt"
    题名 作者 年代 出处 被引量
1Conserved Cytochrome P450 Evolved in Seed Plants Regulates Flower Maturation显示文摘植物染色体的全球检查识别越过 taxa 并且在强壮的净化的选择下面在低拷贝维持的基因,它是可能的有必要功能。基于这个基本原理,我们调查了在种子植物早出现了并且在强壮的否定选择下面演变的低副本的 CYP715 细胞色素 P450 基因家庭的功能。Arabidopsis CYP715A1 在开花期之上在花芽的 tapetum 并且在花药细丝显示出限制织物特定的表情。cyp715a1 插入线在花粉 intine 免职的花瓣开发,和短暂改变显示出一个强壮的缺点。比较表达式分析揭示了涉及花粉开发,房间墙生物的续生说,荷尔蒙动态平衡,和花的 sesquiterpene 的基因的 downregulated 表达式生合成,特别 TPS21 和调整象 MYB21, MYB24,和 MYC2 那样的花的开发的几关键基因。因此,花的 sesquiterpene 排放在 cyp715a1 异种被压制。另外,花荷尔蒙介绍显示了 gibberellinhomeostasis 和 jasmonic 酸衍生物的周转的强壮的骚乱的修正。花瓣生长被活跃赤霉素 GA 3 或 jasmonoyl 异白氨酸的功能的类似物部分恢复, coronatine。CYP715 看起来作为花成熟的一个关键管理者工作,同步花瓣扩大和不稳定的排放。是一个重要决定因素 offlower 昆虫相互作用因此被期望。Zhenhua Liu Benoit Boachon RaphaelLugan Raquel Tavares Mathieu Erhardt Jerome Mutterer Valerie Demais Stephanie Pateyron Veronique Brunaud Toshiyuki Ohnishi Ales Pencik Patrick Achard Fan Gong Peter Hedden Daniele Werck-Reichhart Hugues Renault 2015Molecular Plant2015,8,12:3
2A Non-canonical Transferred DNA Insertion at the BRI1 Locus in Arabidopsis thaliana显示文摘Agrobacterium-mediated transformation is widely used in transgenic plant engineering and has been proven to be a powerful tool for insertional mutagenesis of the plant genome.The transferred DNA(T-DNA) from Agrobacterium is integrated into the plant genome through illegitimate recombination between the T-DNA and the plant DNA.Contrasting to the canonical insertion,here we report on a locus showing a complex mutation associated with T-DNA insertion at the BRI1 gene in Arabidopsis thaliana.We obtained a mutant line,named salade for its phenotype of dwarf stature and proliferating rosette.Molecular characterization of this mutant revealed that in addition to T-DNA a non-T-DNA-localized transposon from bacteria was inserted in the Arabidopsis genome and that a region of more than 11.5 kb of the Arabidopsis genome was deleted at the insertion site.The deleted region contains the brassinosteroid receptor gene BRI1 and the transcription factor gene WRKY13.Our finding reveals non-canonical T-DNA insertion,implicating horizontal gene transfer and cautioning the use of T-DNA as mutagen in transgenic research.Zhong Zhao Yan Zhu Mathieu Erhardt Ying Ruan Wen-Hui Shen 2009Journal of Integrative Plant Biology2009,51,4:2
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