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| 1 | Effect of process parameters on additive-free electrospinning of regenerated silk fibroin nonwovens显示文摘Silk fibroin is a biomaterial with multiple beneficial properties for use in regenerative medicine and tissue engineering.When dissolving and processing the reconstituted silk fibroin solution by electrospinning,the arrangement and size of fibers can be manifold varied and according fiber diameters reduced to the nanometer range.Such nonwovens show high porosity as well as potential biocompatibility.Usually,electrospinning of most biomaterials demands for the application of additives,which enable stable electrospinning by adjusting viscosity,and are intended to evaporate during processing or to be washed out afterwards.However,the use of such additives increases costs and has to be taken into account in terms of biological risks when used for biomedical applications.In this study,we explored the possibilities of additive-free electrospinning of pure fibroin nonwovens and tried to optimize process parameters to enable stable processing.We used natural silk derived from the mulberry silkworm Bombyx mori.After degumming,the silk fibroin was dissolved and the viscosity of the spinning solution was controlled by partial evaporation of the initial solving agent.This way,we were able to completely avoid the use of additives and manufacture nonwovens,which potentially offer higher biocompatibility and reduced immunogenicity.Temperature and relative humidity during electrospinning were systematically varied(25–35°C,25–30%RH).In a second step,the nonwovens optionally underwent methanol treatment to initiate beta-sheet formation in order to increase structural integrity and strength.Comprehensive surface analysis on the different nonwovens was performed using scanning electron microscopy and supplemented by additional mechanical testing.Cytotoxicity was evaluated using BrdU-assay,XTT-assay,LDH-assay and live-dead staining.Our findings were,that an increase of temperature and relative humidity led to unequal fiber diameters and defective nonwovens.Resistance to penetration decreased accordingly.The most uniform fiber diameters of 998±63 nm were obtained at 30°C and 25%relative humidity,also showing the highest value for resistance to penetration(0.20 N).The according pure fibroin nonwoven also showed no signs of cytotoxicity.However,while the biological response showed statistical evidence,the material characteristics showed no statistically significant correlation to changes of the ambient conditions within the investigated ranges.We suggest that further experiments should explore additional ranges for temperature and humidity and further focus on the repeatability of material properties in dependency of suitable process windows. | Alexander Kopp Ralf Smeets Martin Gosau Nadja Kröger Sandra Fuest Marius Köpf Magnus Kruse Judith Krieger Rico Rutkowski Anders Henningsen Simon Burg | 2020 | Bioactive Materials2020，5，2： | 3 |
| 2 | Metabolism and excretion of 14C atorvastatin in patients with T-tube drainage显示文摘 | Martin PF Pond SM | 1996 | Proc Aust Soc Clin Exp Pharmacol Toxicol1996，3，2： | 1 |
| 3 | Failed newborn hearing screens presentation for otitis media with effusion in the newborn population显示文摘 | Boone RT Bower CM Martin PF | 2005 | Int J Pediatr Otorbinolaryngol2005，69，： | 1 |
| 4 | E ffect of a dental air turbine dirll on the bacterial counts in air显示文摘 | Larato DC Ruskin PF Martin A | 1966 | J Prosthet Dent1966，16，4： | 1 |
| 5 | Familial nonmedullary thyroid carcinoma(FNMTC):analysis of fPTC/PRN,NMTC1,MNG1 and TCO susceptibility loci and identification of somatic BRAF and RAS mutations显示文摘 | Cavaco BM Batista PF Martins C | 2008 | Endocr Relat Cancer2008，15，1： | 1 |
| 6 | Fam- ilial non-medullary thyroid carcinoma (FNMTC): analysis of fPTC/PRN, NMTC 1, MNG 1 and TCO susceptibility- loci and identification of somatic BRAF and RAS mutations显示文摘 | Cavaco BM Batista PF Martins C | 2008 | Endocrine Related Cancer2008，15，1： | 1 |
| 7 | Estimation of resting energy expenditure considering effects of race and diabetes status显示文摘 | Martin K Rust PF Wallace P | 2004 | Diabetes Care2004，27，6： | 1 |
| 8 | Cytokine mRNA expression during experimental corneal allograft rejection显示文摘 | Torres PF De Vos AF Van der Gaag R Martins B Kijlstra A | 1996 | Exp Eye Res1996，63，： | 1 |
| 9 | Cytokine mRNA expression during experimental corneal allograft rejection显示文摘 | Torres PF De Vos AF Van der Gaag R Martins B Kijistra A | 1996 | Exp Eye Res1996，63，： | 1 |
| 10 | Cytokine mRNA expression during experimental corneal allograft rejection显示文摘 | Torres PF Devos AF Van der Gaag R Martins B Kijlstra A | 1996 | Exp Eye Res1996，63，4： | 1 |
| 11 | The expression of a new variant of the pro-apoptotic molecule Bax, Baxpsi, is correlated with an increased survival of glioblastoma multiforme patients 显示文摘 | Cartron PF ()liver L Martin S | 2002 | Hum Mol Genet2002，11，6： | 1 |
| 12 | Familial nonmedullary thy- roid carcinoma (FNMTC): analysis of Fptc/PRN, NMTC1, MNG1 and TCO susceptibility loci identi fi cation of somatic BRAF and RAS mutations显示文摘 | Cavaco BM Batista PF Martins C | 2008 | Endocr Relat Cancer2008，15，1： | 1 |
| 13 | Psychometric properties of the functions and addictive features scales of the Ottawa self- injury inventory: a preliminary investigation using a university sample显示文摘 | Martin J Cloutier PF Levesque C | 2013 | PsycholAssess2013，25，3： | 1 |
| 14 | Cytokine m RNA expression during experimental corneal allograftrejection显示文摘 | Torres PF Devos AF Van der Gang R Martins B Kijlstra A | 1996 | Exp Eye Res1996，63，4： | 1 |
| 15 | Cytokine mRNA expression during experimental corneal allograft rejection显示文摘 | Torres PF De Vos AF van der Gaag R Martins B Kijlstra A | 1996 | Exp Eye Res1996，63，4： | 1 |
| 16 | The influence of some fractions of egg yolk on the survival of ram spermatozoa at 5℃ 显示文摘 | Watson PF Martin CA | 1975 | J Biol Sci1975，，28： | 1 |
| 17 | Sartorius myoplasty for deep groin wounds following vascular reconstl'uction显示文摘 | Petrasek PF Kalman PG Martin RD | 1990 | AmJ SurE1990，160，2： | 1 |
| 18 | Optimizing dose selection with modeling and simulation:application to the vasopeptidase inhibitor M100240显示文摘 | Isterm PF Martin NE Haskell LP | 2004 | J Clin Pharmacol2004，44，： | 1 |
| 19 | Effects of acute microinjeetions of thyroid hormone to the-' preopti~, region of euthyroid adult male rats on sleep and motor activity显示文摘 | Martin JV Giannopoulos PF Moffett SX | 2013 | Brain Res2013，1516，26： | 1 |
| 20 | Familial nonmedullary thyroid carcinoma (FNMTC):analysis of fPTC/PRN,NMTC1,MNGI and TCO susceptibility loci and identification of somatic BRAF and RAS mutations显示文摘 | Cavaco BM Batista PF Martins C | 2008 | Endocr Relat Cancer2008，15，1： | 1 |
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