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您的检索式：作者名="MarkA.Feitelson"
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| 1 | Inhibitory effect of tumor suppressor p33^(ING1b) and its synergy with p53 gene in hepatocellular carcinoma显示文摘AIM: To investigate the inhibitory effect of tumor suppressor p33ING1b and its synergy with p53 gene in hepatocellular carcinoma (HCC).METHODS: Recombinant sense and antisense p33ING1b plasmids were transfected into hepatoma cell line HepG2 with lipofectamine. Apoptosis, G0/G1 arrest, cell growth rate and cloning efficiency in soft agar of HepG2 were analyzed after transfection. In three hepatoma cell lineswith different endogenous p53 gene expressions, the synergistic effect of p33ING1b with p53 was analyzed by flow cytometry and luciferase assay was performed to detect the activation of p53 downstream gene p21WAF1/CIP1. In addition, the expression and mutation rates of p33ING1b in HCC tissues were measured by immunohistochemistry and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP).RESULTS: Overexpression of p33ING1b inhibited cell growth of HepG2, induced more apoptosis and protected cells from growth in soft agar. Combined transfer of p33ING1b and p53 gene promoted hepatoma cell apoptosis, G0/G1 arrest and elevated expression of p21WAF1/CIP1. Immunostaining results showed co-localized P33ING1b with P53 protein in HCC tissues and there was a significant relation between protein expression rates of these two genes (P<0.01).Among 28 HCC samples, p33ING1b presented a low gene mutation rate (7.1%).CONCLUSION: p33ING1b collaborates with p53 in cell growth inhibition, cell cycle arrest and apoptosis in HCC. Loss or inactivation of p33ING1b normal function may be an important mechanism for the development of HCC retaining wildtype p53. | ZhiZhu JingLin Jian-HuiQu MarkA.Feitelson Can-RongNi Fang-MeiLi Ming-HuaZhu | 2005 | World Journal of Gastroenterology2005，11，13： | 10 |
| 2 | 乙型肝炎病毒基因组中p53应答成分结合序列的确定及意义显示文摘目的:探讨HBV与肿瘤抑制基因p53相互作用的机制及其在原发性肝癌发生中的意义.方法:应用计算机程序对HBV全基因组进行比较分析,合成含DNA-蛋白质结合位点的基因片段作为探针,与肝癌细胞核蛋白作用.通过凝胶电泳移动试验,凝胶电泳超移动试验,原位紫外线交联试验,确定HBV与p53蛋白的特异性结合.应用带报道基因CAT与p53,HBVX基因共转染,观察HBV与p53蛋白相互作用的生物学功能.结果:HBV基因组增强子I上游区域(1047~1059)存在p53基因应答成份结合位点序列,能特异性地与p53蛋白结合,使p53蛋白在细胞内积聚.结论:HBV与p53存在DNA-蛋白质结合关系,在HBV感染相关的肝癌发生中具有非常重要的理论意义. | 朱明华 MarkA.Feitelson RogerJ.Pomerantz | 1997 | 第四军医大学学报1997，18，5： | 1 |
| 3 | 慢性肝炎、肝硬变和原发性肝癌内HBxAg的表达及意义显示文摘为探讨HBxAg在慢性肝炎、肝硬变和原发性肝癌病变发生中的意义,作者以ABC和PAP免疫组化法研究了HBxAg以及HBsAg和HBcAg在肝及肝癌组织内的存在与分布。在110例慢性肝炎中,72(65.5%)例显示HBxAg阳性,66(60%)例HBsAg和35(31.8%)例HBcAg阳性。在108例肝硬变中,84(77.8%)例显示HBxAg阳性,73(67.6%)例HBsAg和18(16.7%)例HBcAg阳性。在110例原发性肝癌病例中,64(58.2%)例在肝癌组织内显示HBxAg阳性,17(15.5%)例HBsAg和12(10.9%)例HBcAg阳性;在80例癌周肝组织内,63(78.8%)例显示HBxAg阳性,47(58.8%)例HBsAg和21(26.3%)例HBcAg阳性。上述结果表明,在慢性肝炎、肝硬变和肝癌内HBxAg的检出率比HBsAg和HBcAg高,在肝癌组织内尤为明显。本研究证明了慢性肝炎、肝硬变及原发性肝癌和HBV慢性感染有密切关系。 | 王文亮 W.ThomasLondon MarkA.Feitelson | 1992 | 第四军医大学学报1992，13，2： | 1 |
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