|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 乙型肝炎相关肝癌的癌前病变标志物检测及其意义显示文摘目的探讨乙型肝炎相关的肝癌前病变标志物及其临床意义。方法在X基因阳性与阴性的HepG2细胞系中,通过抑制性cDNA差减杂交,建立特异性ELISA方法,对深圳及美国的韩国裔移民730人血清检测肝癌变前标志物抗体(抗-URG4、抗-URG7、抗-URG11、抗-URG12、抗-VEGFR3和抗-Suil)。730人中HBsAg阴性者416名、慢性HBV感染者298例、其他肝炎患者16例。另外对其中53例乙型肝炎肝硬化患者进行3~5年的临床观察。结果HBsAg阴性者6个抗体阳性率与乙型肝炎肝硬化、肝癌患者比较,差异有统计学意义(P值均〈0.01),而与HBV携带者、非肝癌和非病毒性肝炎患者相比,差异均无统计学意义(P值均〉0.05)。而且,这些抗体发生率在高危人群中,与种族、国家、地区无相关性。临床观察显示:53例乙型肝炎肝硬化患者中28例发展成肝癌,其肝癌发生前抗-URG11和抗-VEGFR3同时阳性者16例(P〈0.05);未发生癌变的25例肝硬化患者中,11例抗-URG7、抗-Suil同时阳性(P〈0.05)。此外,在发生肝癌之前检测到4个或更多抗体的患者,其生存期较短。结论6个抗体可在肝癌发生数月或数年前就能被检测到,提示它们可能是肝癌癌前病变重要的标志物,用它们预测肝癌的发生与预后,可能会使通过血清AFP检测诊断小肝癌提前到诊断癌前病变。 | 童光东 周大桥 贺劲松 肖春玲 刘心亮 周小舟 张希 邢宇锋 吕屏 Mark A.Feitelson | 2007 | 中华肝脏病杂志2007，15，11： | 12 |
| 2 | Small rodent models of hepatitis B and C virus replication and pathogenesis显示文摘The narrow host range of infection supporting the long-term propagation of hepatitis B and C viruses is a major limitation that has prevented a more thorough understanding of persistent infection and the pathogenesis of chronic liver disease(CLD).With hepatitis B virus(HBV),this has been partially overcome by the discovery and characterization of HBV-like viruses in wild animals.With hepatitis C virus(HCV),related Flaviviruses have been used as surrogate systems for such studies.Independent work has developed various mouse strains for the transplantation of human hepatocytes,which are then susceptible to infection with HBV and HCV.Other laboratories have developed transgenic mice that express virus gene products and/or support virus replication.Some HBV transgenic mouse models develop fulminant hepatitis,acute hepatitis,or CLD following adoptive transfer,while others spontaneously develop hepatocellular carcinoma(HCC),as in human infections.Among HCV transgenic mice,most develop no disease,but acute hepatitis has been observed in one model,while HCC appears in another.Other mouse models include the introduction of xenographs that replicate HBV or HCV.Although mice are not susceptible to these viruses,their ability to support virus replication and to develop liver disease characteristic of human infections,provides new opportunities to study pathogenesis and develop novel therapeutics. | Mark A.Feitelson Alla Arzumanyan Marcia M.Clayton | 2012 | 微生物与感染2012，7，2： | 0 |
| 3 | Expression of HBxAg in human chronic hepatitis,cirrhosis and primary hepatic carcinoma and its significance显示文摘The specimens of this study were obtained from 110 cases of chronic hepatitis,108cirrhosis and 110 primary hepatic carcinoma(PHC).Formalin-fixed and paraffin-embedded seetions were stained by ABC method forHBxAg,and by PAP method for HRsAg and HBcAgOf the 110 cases of chronic hepatitis,72(65.5%)were positive HBxAg in the liver cells,66(60%)were postitive in HBsAg and 35(31.8%)in HBcAg.Among the 108 eases of drrhosis,84(77.8%)revealed to be HBxAg positive in the liver cells,73(67.6%)were demonstrated to beHBsAg-positive and 18(16.7%)were shown to be HBcAg-positive.Among the 110 eases of pri-mary hepatic carcinoma,64(58.2%)showed HBxAg-positive reaction in cancerous tissues.Therates of positive HRsAg and HBcAg in tumor tissues were 15.5% and 10.9%,respectively.Six-ty-three(78.8%)of 80 cases of the non-cancerous hepatic tissues displayed HBxAg positivenessand the rates of positive HRsAg and HBcAg in the non-tumor tissues were 47(58.8%)and 21(2.6.3%),respectively.The above-mentioned results sugared that the detection rote of HBxAg inchronic hepatitis,cirrhosis and PHC was higher than that of HBsAg and HBcAg.This studydemonstrates a dose relationship between chronic hepatitis,cirrhosis,PHC and chronic persistentinfection of hepatitis B virus(HBV).Persistent chronic HBV infection plays an important role inthe pathogenesis of chronic hepatitis, cirrhosis and PHC.It is possible that the detection ofHBxAg with anti-HBx could be an additional new diagnostic marker for HBV infection.Howev-er,the role of HBxAg in the pathogenesis of chronic liver diseases needs to be furtherinvestigated. | 王文亮 W.Thomas London Mark A.Feitelson | 1992 | Journal of Medical Colleges of PLA(China)1992，7，2： | 0 |
| 4 | 慢性肝炎和肝硬变内HBxAg分布并与HBsAg和 HBcAg的关系显示文摘作者所用110例慢性肝炎和108例肝硬变标本来自西京医院病理科.10%福尔马林固定,石蜡包埋,HE染色,检测HBxAg用ABC方法,检测HBsAg和HBcAg用PAP方法.在110例慢性肝炎中,72(65.5%)例在肝细胞内显示HBxAg阳性,66(60.0%)例HBsAg和35(31.8%)例HBcAg亦阳性.在108例肝硬变中.84(77.8%)例在肝细胞内显示HBxAg阳性,73(67.6%)例HBsAg和18(16.7%)例HBcAg亦阳性.HBxAg与HBsAg和HBcAg之间的关系:在72例HBxAg阳性的慢性肝炎中,56(77.8%)例伴有HBsAg或(和)HBcAg.在84例HBxAg阳性的肝硬变中,60(71.8%)例伴有HBsAg或(和)HBcAg.所有HBsAg或HBcAg阳性的病例,HBxAg也阳性.结果表明,HBxAg的表达和HBV感染有密切关系.HBxAg的检出率比HBsAg和HBcAg高,因此检测HBxAg可以作为判断HBV感染的指标之一.HBxAg在慢性肝炎或肝硬变的发病中可能起重要作用,但如何起作用有待进一步研究. | 王文亮 W.Thomas London Mark A.Feitelson | 1992 | 第四军医大学学报1992，13，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员