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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Pushing the limits of liver surgery for colorectal liver metastases:Current state and future directions显示文摘Liver surgery for the treatment of colorectal liver metastases is the standard treatment in a dynamic surgical field with many variables that should be considered in a curative intent scenario. Hepatectomy for colorectal liver metastases has undergone constant changes over the last 30 years, including indications until the need for rescue procedures of recurrent and advanced diseases as well as minimally invasive surgery. These advancements in liver surgery have not only resulted from overall improvements in the surgical field but have also resulted from a better understanding of the biological behavior of the disease, liver regeneration, and homeostasis during and after surgery.Improvements in anesthesiology, intensive care medicine, radiology, and surgical devices have correlated with further advancements of hepatectomies. Moreover,changes are still forthcoming, and both fields of augmented reality and artificial intelligence will likely have future contributions in this field in regard to both diagnoses and the planning of procedures. The aim of this editorial is to emphasize several aspects that have contributed to the paradigm shifts in colorectal liver metastases surgery over the last three decades as well as to discuss the factors concerning patient selection and the technical aspects of liver surgery. Finally, this editorial will highlight the promising new features of this surgery for diagnoses and treatments in this field. | Raphael LC Araujo Marcelo M Linhares | 2019 | World Journal of Gastrointestinal Surgery2019，11，2： | 3 |
| 3 | Regulation of endothelial cell differentiation and specification显示文摘 | Marcelo KL Goldie LC Hirschi KK | 2013 | Circ Res2013，112，9： | 1 |
| 4 | Optimum size in grid soil sampling for variable rate application in site-specific management显示文摘 | Marcos RN Fabricio PP Jose AMD Roney BO Marcelo LC Everson C | 2011 | Scientia Agricola2011，63，3： | 1 |
| 5 | Regulation of endothelial cell differentiation and specification 显示文摘 | Marcelo KL Goldie LC Hirschi KK | 2013 | Circ Res2013，112，9： | 1 |
| 6 | Conservative surgical treatment of anterior mitral valve aneurysm secondary to aortic valve endocarditis显示文摘 | Marcelo LC Poblo M Limbert LS | | 0，，： | 1 |
| 7 | Regulation of endothelial cell differentiation and specification 显示文摘 | Marcelo KL Goldie LC Hirschi KK | 2013 | Circ Res2013，112，9： | 1 |
| 8 | Oxytocinergic regula- tion of cardiovascular function: studies in oxytocin-deficient mice 显示文摘 | Michelini LC Marcelo MC Amico J | 2003 | Am J Physiol Heart Circ Physiol2003，284，6： | 1 |
| 9 | Regulation of endothe- lial cell differentiation and specification 显示文摘 | Marcelo KL Goldie LC Hirschi KK | 2013 | Circulation Re- search2013，112，9： | 1 |
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