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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | 青藏高原羌塘中部蓝片岩的地球化学特征及其构造意义显示文摘青藏高原羌塘中部的冈玛日 -桃形错地区蓝片岩被认为是板块构造边界的产物 ,通过对其主量元素、微量元素和稀土元素的地球化学特征的综合研究 ,其原岩属于洋岛型碱性玄武岩。再结合该地区的地质研究 ,表明在该地区存在一个古特提斯洋 。 | 邓希光 丁林 刘小汉 An Yin Paul A KAPP Michael A MURPHY Craig E MANNING | 2002 | 岩石学报2002，18，4： | 58 |
| 3 | Schistosoma mansoni proteins attenuate gastrointestinal motility disturbances during experimental colitis in mice显示文摘AIM:To investigate the therapeutic effect of Schistosoma mansoni(S.mansoni) soluble worm proteins on gastrointestinal motility disturbances during experimental colitis in mice. METHODS:Colitis was induced by intrarectal injection of trinitrobenzene sulphate(TNBS) and 6 h later,mice were treated ip with S.mansoni proteins.Experiments were performed 5 d after TNBS injection.Inflammationwas quantified using validated inflammation parameters. Gastric emptying and geometric center were measured to assess in vivo gastrointestinal motility.Peristaltic activity of distal colonic segments was studied in vitro using a modified Trendelenburg set-up.Cytokine profiles of T-lymphocytes isolated from the colon were determined by real time reverse transcriptase-polymerase chain reaction. RESULTS:Intracolonic injection of TNBS caused severe colitis.Treatment with S.mansoni proteins significantly ameliorated colonic inflammation after 5 d.TNBS did not affect gastric emptying but significantly decreased the geometric center and impaired colonic peristaltic activity 5 d after the induction of colitis.Treatment with S.mansoni proteins ameliorated these in vivo and in vitro motility disturbances.In addition,TNBS injection caused a downregulation of effector T cell cytokines after 5 d,whereas a S.mansoni protein effect was no longer observed at this time point. CONCLUSION:Treatment with S.mansoni proteins attenuated intestinal inflammation and ameliorated motility disturbances during murine experimental colitis. | Nathalie E Ruyssers Benedicte Y De Winter Joris G De Man Natacha D Ruyssers Ann J Van Gils Alex Loukas Mark S Pearson Joel V Weinstock Paul A Pelckmans Tom G Moreels | 2010 | World Journal of Gastroenterology2010，16，6： | 11 |
| 4 | Heuristic solutions for the multiple-choice multi-dimension knapsack problem显示文摘 | Akbar M M Manning E G Shoja G C | 2001 | Lecture Notes in Computer Science2001，2074，2： | 1 |
| 5 | The effect test method and surface condition on pitting potential of single and duplex phase 304 L stainless steel 显示文摘 | Manning P E Duquette D Jand Savage W F | 1979 | Corroion1979，35，4： | 1 |
| 6 | Knowledge Management: Strategy or Software Management显示文摘 | Man J Beckett Charles E R Wainwright David Bance | | Decision0，38，： | 1 |
| 7 | Development of a quan titative real-time detection assay for hepatitis B virus DNA and comparison with two commercial assay s显示文摘 | Pas sd Fries E De Man R A | 2000 | Clin Microbiol2000，39，8： | 1 |
| 8 | Entrepreneurial competencies and the performance of small and medium enterprises: an investi- gation through a framework of competitiveness 显示文摘 | Man T Lan T Snape E | 2008 | Journal of Small Business and Entrepreneurship2008，21，3： | 1 |
| 9 | Characteriza- tion of the interaction between the influenza A virus poly- merase subunit PB1 and the host nuclear import factor Ran- binding protein 5显示文摘 | Hutchinson E C Orr O E Man Liu S | 2011 | J Gen Virol2011，92，8： | 1 |
| 10 | Sulfur bearing capacity of hydrogen sulfide gas 显示文摘 | Swiit S C Manning F C Thompson R E | 1976 | SPEJ1976，4，16： | 1 |
| 11 | Atril ejectionforce:a noninvasive assessment of atrial systolic function显示文摘 | Manning W J Silverman D I Katz S E | 1993 | J Am coll cardiol1993，22，1： | 1 |
| 12 | Prediction of angio-graphic vasospasm after aneurysmal subarachnoid hemor-rhage:value of the Hijdra sum scoring system显示文摘 | Dupont S A Wijdicks E F Man no E M | 2009 | Neurocrit Care2009，11，： | 1 |
| 13 | Early phase differential effects of slow and fast harhell squat training显示文摘 | MORRISSEY M C HAP MAN E A FRYKMAN P N | 1998 | Am J Sports Med1998，26，2： | 1 |
| 14 | Survival and prognostic indicators in hepatitis B surface antigen-positive cirrhosis of the liv- er显示文摘 | Jongh F E Janssen H L de Man R A | 1992 | Gastroenterology1992，103，5： | 1 |
| 15 | Crop salt tolerance-current assessment显示文摘 | Maas E V Hof man G J | | 0，，02： | 1 |
| 16 | A comparative study between transmission electron microscopy and immunofluorescence mapping in the diagnosis of epidermolysis bullosa显示文摘 | Yiasemides E Walton J Man P | 2006 | Am J Dermatopathol2006，28，5： | 1 |
| 17 | Synthesis,structural characterization, experimental, and computational spectrophotometric studies of 8-quinolinyloxymethypho- sphonate compounds 显示文摘 | Man S P Benoit D M Buchaca E | 2006 | Inorg Chem2006，45，： | 1 |
| 18 | Analysis of potential lard adulteration in chocolate and chocolate products using fourier transform infrared spectroscopy 显示文摘 | Che Man Y B Syahariza Z A Mirghani M E S | 2005 | Food Chemistry2005，90，4： | 1 |
| 19 | Role of placental growth factor (PLGF) in wound healing after glaucoma filtration surgery 显示文摘 | VAN BT VAN DE VEIRE S VANDEWALLE E MOONS L STAL- MANS I | 2011 | Bull Soc Beige Ophtalmo12011，，317： | 1 |
| 20 | A luciferase reporter gene assay and aryl hydrocarbon receptor 1 genotype predict the LD50 of polychlorinated biphenyls in avian species显示文摘 | Manning G E Farmahin R Crump D | 2012 | Toxicology and Applied Pharmacology2012，263，3： | 1 |
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