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3篇 您的检索式:作者名="Mallam Nock Joshua"
    题名 作者 年代 出处 被引量
1杆状病毒p35基因延缓昆虫细胞凋亡显示文摘构建了以新霉素抗性基因为筛选标记的带有p35基因的转移载体p35IE1Neo,以此载体转染Sf9细胞,经G418筛选得到染色体上稳定整合质粒p35IE1Neo的Sf9细胞,克隆化培养后取名为Sf9-35.经细胞原位杂交实验证明,Sf9-35细胞的染色体DNA上含有p35基因;经放线菌素D处理后的细胞核酸电泳检测,证实Sf9-35具有抗凋亡特性能够支持凋亡抑制基因缺失的vAcΔp35病毒复制;发现p35基因在细胞内组成型表达只能延缓vAcΔp35感染及放线菌素D处理诱发的细胞凋亡的过程,而不能完全阻止其诱发的细胞凋亡.李小峰 齐义鹏 林宏 Mallam Nock Joshua 李燕 李志达 1998武汉大学学报(自然科学版)1998,44,4:2
2Nucleotide Sequence of Polyhedrin Gene of LsNPV and Analysis of Baculovirus Polyhedron Proteins显示文摘NucleotideSequenceofPolyhedrinGeneofLsNPVandAnalysisofBaculovirusPolyhedronProteins¥WangJiawang;QiYipeng;DengYanhui;MallamNoo...Wang Jiawang Qi Yipeng Deng Yanhui Mallam Nock Joshua 1996Wuhan University Journal of Natural Sciences1996,1,2:0
3Cloning and Expression of HIV-1 p24 Gene in Insect Cells by Using BAC-TO-BAC System显示文摘Recombinant transposing vector pFHIV24 was constructed by cloning the HIV-1 p24 gene intothe Multiple cloning site (MCS) of the transposing vector pFastBac1 in the correct orientation with respect tothe polyhedrin promoter. Recombinant bacmid bHIV24 was obtained by transposing a mini-att Tn7 elementfrom the recombinant pFHIV24 to the mini-att Tn7 attachment site on the bacmid by Tn7 transposition functions provided by the helper plasmid. Minipreparation of recombinant bacmid DNA was transfected intoSpodoptera frugiperda(Sf9). cells to get the recombinant virus. Fresh insect Sf9 cells were infected with therecombinant virus containing p24 to express the target protein. The target protein expressed was analyzed ona 15% polghcrylamide gels and then used as antigen to check HIV-1 positive serum by ELISA. Our positiveresult shows that the expressed p24 protein could be used as standard antigen for HIV-1 diagnosis by ELISAand other reliable diagnostic methods or HIV-1 Infection.Mallam Nock Joshua Qi Yipeng Huang Yongxiu Liu Ziye 1998Wuhan University Journal of Natural Sciences1998,3,1:0
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