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| 1 | Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification显示文摘AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects (-4.08±0.90 vs-3.08±1.38,P=0.05).Furthermore,a phylotype with 94%similarity to R.torques was more prevalent in IBS-D patients'intestinal micro- biota than in that of control subjects(-2.43±1.49 vs -4.02±1.63,P=0.01).A phylotype with 93%simi- larity to R.torques was associated with control sam- ples when compared with IBS-M(-2.41±0.53 vs -2.92±0.56,P=0.00).Additionally,a R.bromii-like phylotype was associated with IBS-C patients in com- parison to control subjects(-1.61±1.83 vs-3.69± 2.42,P=0.01).All of the above mentioned phylotype specific alterations were independent of the effect of time. CONCLUSION:Significant phylotype level alterationsin the intestinal microbiotas of IBS patients were observed,further emphasizing the possible contribution of the gastrointestinal microbiota in IBS. | Anna Lyra Teemu Rinttil Janne Nikkil Lotta Krogius-Kurikka Kajsa Kajander Erja Malinen Jaana Mtt Laura Mkel Airi Palva | 2009 | World Journal of Gastroenterology2009，15，47： | 26 |
| 2 | Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome显示文摘AIM:To investigate the correlations between selfreported symptoms of irritable bowel syndrome(IBS) and the gastrointestinal(GI) microbiota composition.METHODS:Fecal samples were collected from a total of 44 subjects diagnosed with IBS.Their symptoms were monitored with a validated inflammatory bowel disease questionnaire adjusted for IBS patients.Thirteen quantitative real-time polymerase chain reaction assays were applied to evaluate the GI microbiota composition.Eubacteria and GI bacterial genera(Bifidobacterium,Lactobacillus and Veillonella),groups(Clostridium coccoides/Eubacterium rectale,Desulfovibrio desulfuricans) and distinct bacterial phylotypes [closest 16S rDNA sequence resemblance to species Bifidobacterium catenulatum,Clostridium cocleatum,Collinsella aerofaciens(C.aerofaciens),Coprococcus eutactus(C.eutactus),Ruminococcus torques and Streptococcus bovis ] with a suspected association with IBS were quantified.Correlations between quantities or presence/absence data of selected bacterial groups or phylotypes and various IBSrelated symptoms were investigated.RESULTS:Associations were observed between subjects' self-reported symptoms and the presence or quantities of certain GI bacteria.A Ruminococcus torques(R.torques)-like(94% similarity in 16S rRNA gene sequence) phylotype was associated with severity of bowel symptoms.Furthermore,among IBS subjects with R.torques 94% detected,the amounts of C.cocleatum 88%,C.aerofaciens-like and C.eutactus 97% phylotypes were significantly reduced.Interesting observations were also made concerning the effect of a subject's weight on GI microbiota with regard to C.aerofaciens like phylotype,Bifidobacterium spp.and Lactobacillus spp.CONCLUSION:Bacteria seemingly affecting the symptom scores are unlikely to be the underlying cause or cure of IBS,but they may serve as biomarkers of the condition. | Erja Malinen Lotta Krogius-Kurikka Janne Nikkil Airi Palva Anna Lyra Anne Jskelinen Teemu Rinttil Terttu Vilpponen-Salmela Atte Johannes von Wright | 2010 | World Journal of Gastroenterology2010，16，36： | 13 |
| 3 | Classification of dynamic contrast enhanced MR images of cervical cancers using texture analysis and support vector machine显示文摘 | Torheim T Malinen E Kvaal K | 2014 | IEEE Trans Med Imaging2014，33，8： | 1 |
| 4 | Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syn-drome sufferers显示文摘 | Krogius-Kurikka L Lyra A Malinen E | | 0，，： | 1 |
| 5 | Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real- time PCR显示文摘 | Rinttilfi T Kassinen A Malinen E | 2004 | J Appl Microbiol2004，97，6： | 1 |
| 6 | Comparison of real-timePCR with SYBR Green I or 5'-nuclease assays and dot-blot hy-bridization with r DNA-targeted oligonucleotide probes in quantifi-cation of selected faecal bacteria显示文摘 | Malinen E Kassinen A Rinttila T | 2003 | Microbiology2003，149，1： | 1 |
| 7 | Differential tran- scriptional response of Bifidobacterium longum to human milk, formula milk, and galactooligosaccharide显示文摘 | Gonzhlez R Klaassens ES Malinen E | 2008 | Appl Environ Mi- crobio12008，74，15： | 1 |
| 8 | Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by realtime PCR显示文摘 | Rinttila T Kassinen A Malinen E | 2004 | J Appl Microbiol2004，97，6： | 1 |
| 9 | Comparison of realtime PCR with SYBR Green Ⅰ or 5(n)uclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria显示文摘 | MALINEN E KASSINEN A RINTTILA T | 2003 | Microbiology2003，149，： | 1 |
| 10 | Determination of therelaxation modulus of a linearly viscoelastic material 显示文摘 | Joonas Sorvari Matti Malinen | 2006 | Mechanics of Time-dependent Materials2006，10，2： | 1 |
| 11 | Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR显示文摘 | Rinttil?T Kassinen A Malinen E | | 0，，06： | 1 |
| 12 | The number of vitamin D receptor binding sites defines the different vitamin D responsiveness of the CYP24 gene in malignant and normal mammary ceils显示文摘 | Matilainen JM Malinen M Turunen MM | 2010 | J Biol Chem2010，285，24： | 1 |
| 13 | Comparison of realtime PCR with SYBR Green I or 5'nuclease assays and dot-blot hybridization with RDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria显示文摘 | MALINEN E KASSINEN A RINTTILA T | 2003 | 149:269-2772003，149，： | 1 |
| 14 | Genetic labeling of lactobacilli in a food grade manner for strain-specific detection of industrial starlets and probiotic strains显示文摘 | EFIA MALINEN REIJA LAITINCN AIRI PALVA | 2001 | Food Microbilogy2001，，18： | 1 |
| 15 | Development of an extensive set of 16S rDNA-targeted pri-mers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR显示文摘 | Rinttila T Kassinen A Malinen E | 2004 | Journal of Ap- plied Microbiology2004，97，： | 1 |
| 16 | Comparison of real-time PCR with SYBR Green I or 5'nuclease assays and dot-blot hybridization with rDNA-targeted oligonueleotide probes in quantification of' selected faecal bacteria显示文摘 | Malinen E Kassinen A Rinttila T | 2003 | Microbiology2003，149，1： | 1 |
| 17 | Microbial Community Analysis Reveals High Level Phylogenetie Alterations in the Overall Gastroin- testinal Miexxbiota of Diarrhoea-Predominant Irritable Bowel Syn- drome Sufferers显示文摘 | Kurikka LK Lyra A Malinen E | 2009 | OMC Gastroenterology2009，9，12： | 1 |
| 18 | LED-Based NIR Spectrometer Module for Hand-Held and Process Analyser Applications显示文摘 | Malinen J Kansakoski M Rikola R Eddison C G | 1998 | Sensor and Actuators B1998，51，13： | 1 |
| 19 | Comparison of realtime PCR with SYBR Green Ⅰ or 59-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria显示文摘 | Malinen E Kassinen A Rinttil(a) T | 2003 | Microbiology2003，149，1： | 1 |
| 20 | Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture显示文摘 | Bhattacharya M Malinen MM Lauren P | 2012 | J Control Release2012，164，3： | 1 |
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