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| 1 | Exploitation of Fenton and Fenton-like reagents as alternative conditioners for alum sludge conditioning显示文摘The use of Fenton's reagent(Fe2+/H2O2) and Fenton-like reagents containing transition metals of Cu(II) ,Zn(II) ,Co(II) ,and Mn(II) for an alum sludge conditioning to improve its dewaterability was investigated. The results obtained were compared with those obtained from conditioning the same alum sludge using cationic and anionic polymers. Experimental results show that Fenton's reagent was the best among the Fenton and Fenton-like reagents for the alum sludge conditioning. A considerable effiectiveness of capillary suction time(CST) reduction efflciency of 47% can be achieved under test conditions of Fe2+/H2O2 = 20/125 mg/g DS(dry solid) and pH 6.0. The observation of floc-like particles after Fenton's reagent conditioning of alum sludge suggested that the mechanism of Fenton's reagent conditioning was different from that of polymer conditioning. In spite of the lower efflciency in the CST reduction of Fenton's reagent in alum sludge conditioning compared to that of polymer conditioning,Fenton's reagent o?ers a more environmentally safe option. This study provided an example of proactive treatment engineering,which is aimed at seeking a safe alternative to the use of polymers in sludge conditioning towards achieving a more sustainable sludge management strategy. | Maha A. Tony Y. Q. Zhao Aghareed M. Tayeb | 2009 | Journal of Environmental Sciences2009，21，1： | 26 |
| 2 | High fat diet dysregulates microRNA-17-5p and triggers retinal inflammation:Role of endoplasmic-reticulum-stress显示文摘AIM To elucidate how high diet-induced endoplasmic reticulum-stress upregulates thioredoxin interacting protein expression in Müller cells leading to retinal inflammation. METHODS Male C57Bl/J mice were fed either normal diet or 60% high fat diet for 4-8 wk. During the 4 wk study, mice received phenyl-butyric acid(PBA); endoplasmic reticulum-stress inhibitor; for 2 wk. Insulin resistance was assessed by oral glucose tolerance. Effects of palmitate-bovine serum albumin(BSA)(400 μmol/L) were examined in retinal Müller glial cell line and primary Müller cells isolated from wild type and thioredoxin interacting protein knock-out mice. Expression of thioredoxin interacting protein, endoplasmic reticulum-stress markers, mi R-17-5p m RNA, as well as nucleotide-binding oligomerization domain-like receptor protein(NLRP3) and IL1β protein was determined.RESULTS High fat diet for 8 wk induced obesity and insulin resistance evident by increases in body weight and impaired glucose tolerance. By performing quantitative real-time polymerase chain reaction, we found that high fat diet triggered the expression of retinal endoplasmic reticulum-stress markers(P < 0.05). These effects were associated with increased thioredoxin interacting protein and decreased mi R-17-5p expression, whichwere restored by inhibiting endoplasmic reticulumstress with PBA(P < 0.05). In vitro, palmitate-BSA triggered endoplasmic reticulum-stress markers, which was accompanied with reduced mi R-17-5p and induced thioredoxin interacting protein m RNA in retinal Müller glial cell line(P < 0.05). Palmitate upregulated NLRP3 and IL1β expression in primary Müller cells isolated from wild type. However, using primary Müller cells isolated from thioredoxin interacting protein knock-out mice abolished palmitate-mediated increase in NLRP3 and IL1β.CONCLUSION Our work suggests that targeting endoplasmic reticulumstress or thioredoxin interacting protein are potential therapeutic strategies for early intervention of obesityinduced retinal inflammation. | Maha Coucha Islam N Mohamed Sally L Elshaer Osinakachuk Mbata Megan L Bartasis Azza B El-Remessy | 2017 | World Journal of Diabetes2017，8，2： | 8 |
| 3 | Role of AXL in invasion and drug resistance of colon and breast cancer cells and its association with p53 alterations显示文摘AIM To characterize AXL receptor tyrosine kinase(AXL)expression in relationship to tumor protein P53(TP53gene,p53 protein)and its role in tumor invasion and response to therapy.METHODS We used 14 cell lines,including 3 isogenic pairs carrying mutant/knockout p53,to gain insight into the relationship between AXL and TP53.These included HCT116,HCT116.p53 mutant,RKO,and RKO.p53-/-lines(all from colon cancers)as well as breast cancer cell lines MCF7 and 1001(MCF7-p53 mutant clone).He La cell line was used as a positive control for epithelial to mesenchymal transition(EMT).AXL expression was determined by Western blotting using rabbit monoclonal antibody clone C89E7.AXL si RNA silencing was performed and followed by collagen invasion assay.Cell viability analysis using the sulforhodamine B assay and the invasion assay were performed after exposure to chemotherapeutic agents(doxorubicin for breast cancer cells;5FU or irinotecan for colon cancer cells).RESULTS We showed that the introduction of p53 mutations or knockout increased expression levels of AXL in isogenic cells compared to the matching p53 wild-type parental cells.Overall,we found a trend for correlation between the potential EMT candidate AXL,p53 alterations,and EMT markers in colorectal and breast cancers.The expression of AXL in RKO cells,a rare colon cancer cell line with inactive Wnt signaling,suggests that the AXL oncogene might provide an alternative genetic pathway for colorectal carcinogenesis in the absence of Wnt signaling activation and TP53 mutation.AXL silencing in the TP53 mutant isogenic cell lines 1001,HCT116.p53 mutant and RKO.P53-/-was>95%efficient and the silenced cells were less invasive compared to the parental TP53 wild-type cells.AXL silencing showed a subtle trend to restore colon cancer cell sensitivity to5FU or irinotecan.Importantly,AXL expressing cells developed more invasive potential after exposure to chemotherapy compared to the AXL-silenced cells.CONCLUSION AXL is influenced by p53 status and could cause the emergence of aggressive clones after exposure to chemotherapy.These findings could have applications in cancer management. | Wael M Abdel-Rahman Noura A Al-khayyal Vidhya A Nair S R Aravind Maha Saber-Ayad | 2017 | World Journal of Gastroenterology2017，23，19： | 7 |
| 4 | Evaluation of patients with dry eye disease for conjunctival Chlamydia trachomatis and Ureaplasma urealyticum显示文摘AIM:To determine the possibility of the development of dry eye disease(DED) as a result of persistent infection with Chlamydia trachomatis and Ureaplasma urealyticum in the conjunctiva of patients.METHODS: This study was conducted on 58 patients of age range 20-50 y,diagnosed with DED confirmed by Schirmer I test and tear breakup time.The non-dry eye control group included 27 subjects of the same age.Ocular specimens were collected as conjunctival scrapings and swabs divided into three groups: the first used for bacterial culture,the second and third taken to detect Chlamydia trachomatis and Ureaplasma urealyticum by direct fluorescent antibody(DFA) assay and polymerase chain reaction(PCR) method. RESULTS: Chlamydia trachomatis was detected in 65.5% and 76% of DED patients by DFA and PCR methods respectively.Ureaplasma urealyticum was found in 44.8% of DED infected patients using the PCR method.Both organisms were identified in only 37.9% of DED patients found to be infected.Control subjects had a 22%detection rate of Chlamydia trachomatis by DFA assay versus a 7% detection rate by PCR; while Ureaplasma urealyticum was detected in 3.7% of the controls by PCR method.The conjunctival culture revealed that gram positive microorganisms represented 75% of isolates with coagulase negative Staphylococci the most common(50%) followed by Staphylococcus aureus(20%),whereas gram negative microorganisms occurred in 25% of cases,isolating Moraxella spp.as the most frequent organism. CONCLUSION: Our results tend to point out that Chlamydia trachomatis and Ureaplasma urealyticum were detected in a moderate percentage of patients with DED,and could be a fair possibility for its development.PCR is more reliable in detecting Chlamydia trachomatis than DFA technique.The presence of isolated conjunctival bacterial microflora can be of some potential value. | Maha Mohssen Abdelfattah Rania Abdelmonem Khattab Magda H.Mahran Ebrahim S.Elborgy | 2016 | International Journal of Ophthalmology(English edition)2016，9，10： | 6 |
| 5 | Overview of recent advances in metastatic triple negative breast cancer显示文摘Metastatic triple negative breast cancer(TNBC)has an aggressive phenotype with a predilection for visceral organs and brain.Best responses to chemotherapy are predominately in the first line.Recent studies have demonstrated improved progression free survival with the combination of atezolizumab/pembrolizumab and chemotherapy in programmed death-ligand 1 positive metastatic TNBC.However,a recent trial in a similar population showed no benefit for atezolizumab and paclitaxel which led to a Food and Drug Administration alert.Two phase III trials(OLYMPIAD and BROCADE3)demonstrated a benefit in progression free survival(PFS)but not overall survival in patients with BRCAassociated metastatic TNBC treated with Olaparib or Talazoparib respectively.For those treated with Talazoparib,the time to deterioration in health related-quality of life was also longer compared to chemotherapy.The BROCADE3 trial demonstrated that the combination of a platinum and veliparib increased PFS in first-line metastatic TNBC but at the cost of increased toxicity.There are no headto-head comparisons of a poly(adenosine diphosphate-ribose)polymerase inhibitors(PARPi)and platinums.There are unanswered questions regarding the role of PARPi maintenance after platinum therapy as is standard of care in BRCAassociated ovarian cancer.Other areas of therapeutic interest include targeting aberrations in the phosphoinositide 3-kinase pathway,protein kinase B,mammalian target of rapamycin or utilising antibody drug conjugates.This review focusses on recent and emerging therapeutic options in metastatic TNBC.We searched PubMed,clinicaltrials.gov and recent international meetings from American Society of Clinical Oncology,San Antonio Breast Cancer Conference and the European Society of Medical Oncology. | David O'Reilly Maha Al Sendi Catherine M Kelly | 2021 | World Journal of Clinical Oncology2021，12，3： | 5 |
| 6 | Compliant plant wearables for localized microclimate and plant growth monitoring显示文摘The microclimate surrounding a plant has major effect on its health and photosynthesis process,where certain plants struggle in suboptimal environmental conditions and unbalanced levels of humidity and temperature.The ability to remotely track and correlate the effect of local environmental conditions on the healthy growth of plants can have great impact for increasing survival rate of plants and augmenting agriculture output.This necessitates the widespread distribution of lightweight sensory devices on the surface of each plant.Using flexible and biocompatible materials coupled with a smart compact design for a low power and lightweight system,we develop widely deployed,autonomous,and compliant wearables for plants.The demonstrated wearables integrate temperature,humidity and strain sensors,and can be intimately deployed on the soft surface of any plant to remotely and continuously evaluate optimal growth settings.This is enabled through simultaneous detection of environmental conditions while quantitatively tracking the growth rate(viz.elongation).Finally,we establish a nature-inspired origami-assembled 3D-printed“PlantCopter”,used as a launching platform for our plant wearable to enable widespread microclimate monitoring in large fields. | Joanna M.Nassar Sherjeel M.Khan Diego Rosas Villalva Maha M.Nour Amani S.Almuslem Muhammad M.Hussain | 2018 | npj Flexible Electronics2018，2，1： | 5 |
| 7 | Helicobacter pylori may be an initiating factor in newly diagnosed ulcerative colitis patients: A pilot study显示文摘AIM To directly visualize Helicobacter pylori(H. pylori) by the highly sensitive and specific technique of immunohistochemical staining in colonic tissue from patients newly diagnosed with ulcerative colitis(UC).METHODS Colonoscopic biopsies from thirty patients with newly diagnosed UC and thirty controls were stained with Giemsa stain and immunohistochemical stain for detection of H. pylori in the colonic tissue. Results were confirmed by testing H. pylori Ag in the stool then infected patients were randomized to receive either anti H. pylori treatment or placebo.RESULTS Twelve/30(40%) of the UC patients were positive for H. pylori by Giemsa, and 17/30(56.6%) by immunohistochemistry stain. Among the control group 4/30(13.3%) and 6/30(20 %) were positive for H. pylori by Giemsa and immunohistochemistry staining respectively. H. pylori was significantly higher in UC than in controls(P = 0.04 and 0.007). All Giemsa positive patients and controls were positive by immunohistochemical stain. Four cases of the control group positive for H. pylori also showed microscopic features consistent with early UC.CONCLUSION H. pylori can be detected in colonic mucosa of patients with UC and patients with histological superficial ulcerations and mild infiltration consistent with early UC. There seems to be an association between UC and presence of H. pylori in the colonic tissue. Whether this is a causal relationship or not remains to be discovered. | Loai Mansour Ferial El-Kalla Abdelrahman Kobtan Sherief Abd-Elsalam Mohamed Yousef Samah Soliman Lobna Abo Ali Walaa Elkhalawany Ibrahim Amer Heba Harras Maha M Hagras Mohamed Elhendawy | 2018 | World Journal of Clinical Cases2018，6，13： | 4 |
| 8 | Cytokine profile in Egyptian hepatitis C virus genotype-4 in relation to liver disease progression显示文摘AIM: To observe the imbalance between T helper cell Th1 and Th2 cytokines in several chronic hepatitis disease at different stages of disease progression.METHODS: We measured the cytokine levels of Th1 (IL-2 and IL-2R), Th2 (IL-10) and the pro-inflammatory cytokines (IL-6 and IL-6R and TNF and TNF-RⅠ and Ⅱ)by the ELISA technique in the sera of 33 hepatocellular carcinoma (HCC) patients and 20 chronic liver disease (CLD) patients. In addition, 20 asymptomatic hepatitis C virus carriers and 20 healthy subjects negative for hepatitis C virus(HCV) markers served as controls.RESULTS: Anti-HCV antibodies were found to be positive in 94% of HCC cases and 75% of CLD cases.On the other hand, HCV viremia was detected using RTPCR in 67% of HCC cases and 65% of CLD cases. HBsAg was positive in 9% of HCC cases and 30% of CLD cases.Also bilharzial-Ab was positive in 55% of HCC cases,65% of CLD cases and in 70% of asymptomatic carriers (ASC). HCC patients had significantly higher values of IL-2R, TNF-RⅡ (P<0.001), and TNF-RⅠ (P>0.05), but lower TNFα (P<0.001) and IL-6 (P = 0.032) in comparison to ASC. But, in comparison to non-cancer controls, HCC patients had higher values of IL-2R, IL-6R, TNF-RⅠ and TNF-RⅡ, but lower TNF-α (P<0.001). CLD patients had higher IL-2R, TNF-RⅠ, and TNF-RⅡ (P<0.001) than ASC. But, in comparison to non-cancer controls, CLD patients had higher values of IL-2R, TNF-RⅠ and TNF-RⅡ, but lower TNF-α (P<0.001). IL-10 was higher (though not significantly) in HCC and CLD patients than in symptomatic carriers and non-cancer controls.CONCLUSION: Liver disease progression from CLD to HCC due to HCV genotype-4 infection is associated with an imbalance between Th1 and Th2 cytokines. IL-2R,TNF-RⅠ, and TNF-RⅡ could be used as potential markers. | Abdel-Rahman N Zekri Mohammed S El-Din Ashour Ahmed Hassan Hanaa M Alam El-Din Amal MR El-Shehaby Maha A Abu-Shady | 2005 | World Journal of Gastroenterology2005，11，42： | 4 |
| 9 | 电化学氢压缩和纯化与竞争技术的对比:Ⅱ.电催化的挑战显示文摘在未来的几十年里,氢将成为世界能源政策的基础,因为它的脱碳性质,至少在可再生能源生产时是如此.目前,氢基本上仍然是从化石原料中生产出来的(在某种程度上来自于生物质);因此,目前市场上的氢气含有不可忽略的杂质,这些杂质阻碍了氢气立即用于特殊化学或作为燃料电池的能量载体,例如在运输应用(汽车、公共汽车、火车、船只等)中逐渐扩散到地球上.因此,氢气必须具有足够的纯度,但也必须充分压缩(在高压下,通常为70 MPa),这使得氢气循环中不可避免存在净化和压缩步骤.如本文第一部分'电化学氢压缩和净化与竞争技术的对比--第一部分:优缺点'所示,电化学氢压缩机(EHCs)能够实现氢净化和压缩,具有比它们相应的机械设备更多的理论(热力学)和实际(动力学)的优越性.然而,为了具有竞争力, EHCs必须在非常密集的条件下运行(高电流密度和低电池电压),只有在其核心材料(如膜和电极/电催化剂)经过优化后才能达到.本文将特别关注电催化剂在EHCs中必须表现出的特性:在杂质存在的情况下它们应促进(非常)快速的氢氧化反应(HOR),这意味着它们对毒物也具有(非常)耐受性.这包括操作氢气净化压缩所用的EHC阳极的先决条件,而大量低温燃料电池文献中为耐受毒性而开发的材料可能并不总是满足这两个标准,正如本综述所阐述的. | Marine Tregaro Maha Rhandi Florence Druart Jonathan Deseure Marian Chatenet | 2020 | Chinese Journal of Catalysis2020，41，5： | 4 |
| 10 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 11 | High body mass index is an independent risk factor for nonresponse to antiviral treatment in chronic hepatitis C显示文摘 | Brian L. Bressler Maha Guindi George Tomlinson Jenny Heathcote | 2003 | Hepatology2003，，3： | 3 |
| 12 | Nogo receptor expression in microglia/macrophages during experimental autoimmune encephalomyelitis progression显示文摘Myelin-associated inhibitory factors within the central nervous system(CNS) are considered to be one of the main obstacles for axonal regeneration following disease or injury. The nogo receptor 1(NgR1) has been well documented to play a key role in limiting axonal regrowth in the injured and diseased mammalian CNS. However, the role of nogo receptor in immune cell activation during CNS inflammation is yet to be mechanistically elucidated. Microglia/macrophages are immune cells that are regarded as pathogenic contributors to inflammatory demyelinating lesions in multiple sclerosis(MS). In this study, the animal model of MS, experimental autoimmune encephalomyelitis(EAE) was induced in ngr1^(+/+) and ngr1^(–/–) female mice following injection with the myelin oligodendrocyte glycoprotein(MOG_(35–55)) peptide. A fatemap analysis of microglia/macrophages was performed throughout spinal cord sections of EAE-induced mice at clinical scores of 0, 1, 2 and 3, respectively(increasing locomotor disability) from both genotypes, using the CD11 b and Iba1 cell markers. Western immunoblotting using lysates from isolated spinal cord microglia/macrophages, along with immunohistochemistry and flow cytometric analysis, was performed to demonstrate the expression of nogo receptor and its two homologs during EAE progression. Myelin protein engulfment during EAE progression in ngr1^(+/+) and ngr1^(–/–) mice was demonstrated by western immunblotting of lysates from isolated spinal cord microglia/macrophages, detecting levels of Nogo-A and MOG. The numbers of M1 and M2 microglia/macrophage phenotypes present in the spinal cords of EAE-induced ngr1^(+/+) and ngr1^(–/–) mice, were assessed by flow cytometric analysis using CD38 and Erg-2 markers. A significant difference in microglia/macrophage numbers between ngr1^(+/+) and ngr1^(–/–) mice was identified during the progression of the clinical symptoms of EAE, in the white versus gray matter regions of the spinal cord. This difference was unrelated to the expression of Ng R on these macrophage/microglial cells. We have identified that as EAE progresses, the phagocytic activity of microglia/macrophages with myelin debris, in ngr1^(–/–) mice, was enhanced. Moreover, we show a modulation from a predominant M1-pathogenic to the M2-neurotrophic cell phenotype in the ngr1^(–/–) mice during EAE progression. These findings suggest that CNS-specific macrophages and microglia of ngr1^(–/–) mice may exhibit an enhanced capacity to clear inhibitory molecules that are sequestered in inflammatory lesions. | Amani A.Alrehaili Jae Young Lee Maha M.Bakhuraysah Min Joung Kim Pei-Mun Aui Kylie A.Magee Steven Petratos | 2018 | Neural Regeneration Research2018，13，5： | 3 |
| 13 | 电化学氢压缩和纯化与竞争技术的对比:Ⅰ.优缺点显示文摘毫无疑问,氢将在我们未来的能量组合中发挥重要作用,因为它可以储存可再生电(电-氢)并在燃料电池中可逆地转化为电能,更不用说它在(石油)化学工业中的广泛应用了.然而,在这些应用中需要纯氢,而如今的制氢仍主要基于化石燃料,因而不能被视为纯氢.因此,大规模的氢气净化是必须的.此外,氢是最轻的气体,它的体积能量含量远远低于它的竞争燃料,除非它在高压下被压缩(通常70MPa),使压缩不可避免.本文将详细说明目前可用于氢气净化和压缩的方法.这将表明在现有的技术中,也可以实现氢气净化的电化学氢压缩机(EHC)与目前工业规模上使用的经典技术相比具有许多优势.EHC有其热力学和操作上的优点,但也易于使用.然而,只有达到足够的性能, EHCs的部署才是可行的,这意味着他们的基础材料应遵守一些规范.本文将详述这些规范. | Maha Rhandi Marine Tregaro Florence Druart Jonathan Deseure Marian Chatenet | 2020 | Chinese Journal of Catalysis2020，41，5： | 3 |
| 14 | Validated stability indicating methods for determination of nitazoxanide in presence of its degradation products显示文摘Three sensitive,selective and reproducible stability-indicating methods are presented for determination of nitazoxanide (NTZ),a new anti-protozoal drug,in presence of its degradation products.Method A utilizes the first derivative of ratio spectra spectrophotometry by measurement of the amplitude at 364.4 nm using one of the degradation products as a divisor.Method B is a chemometric-assisted spectrophotometry,where principal component regression (PCR) and partial least squares (PLS) were applied.These two approaches were successfully applied to quantify NTZ in presence of degradation products using the information included in the absorption spectra in the range 260-360 nm.Method C is based on the separation of NTZ from its degradation products followed by densitometric measurement of the bands at 254 nm.The separation was carried out on silica gel 60F 254,using chloroform-methanol-ammonia solution-glacial acetic acid (95:5:1:1 by volume,pH=5.80) as a developing system.These methods are suitable as stability-indicating methods for the determination of NTZ in presence of its degradation products either in bulk powder or in pharmaceutical formulations.Statistical analysis of the results has been carried out revealing high accuracy and good precision. | Nouruddin W.Ali Samah Sayed Abbas Hala El-Sayed Zaazaa Maha Mohamed Abdelrahman Mohamed Abdelkawy | 2012 | Journal of Pharmaceutical Analysis2012，2，2： | 3 |
| 15 | Hypoglycemic and antioxidant activities of Caesalpinia ferrea Martius leaf extract in streptozotocininduced diabetic rats显示文摘Objective: To evaluate the antidiabetic and antioxidant ef ects of aqueous ethanolic extract of Caesalpinia ferrea(C. ferrea) leaf in normal and streptozotocin(STZ) induced diabetic rats.Methods: Male Sprague-Dawley rats divided into 6 groups of 6 rats each were assigned into diabetic and non-diabetic groups. Diabetes was induced in rats by single intraperitoneal administration of STZ(65 mg/kg body weight). C. ferrea extract at the doses of 250 and 500 mg/kg body weight was orally administered to both diabetic and non-diabetic animals for a period of 30 days. After completion of experimental duration serum, liver and pancreas were used for evaluating biochemical and histopathological changes.Results: Oral administration of C. ferrea leaf extract significantly reduced elevated serum glucose, α-amylase, liver function levels and signii cantly increased serum insulin, total protein and body weight as well as improved lipid proi le due to diabetes. Furthermore, the treatment resulted in a marked increase in glutathione peroxidase, superoxide dismutase, catalase and reduced glutathione, and diminished levels of lipid peroxidation in liver and pancreas of diabetic rats. Histopathological studies demonstrated the reduction in the pancreas and liver damage and coni rmed the biochemical i ndings.Conclusions: From the present study, it can be concluded that the C. ferrea leaf extract ef ectively improved hyperglycaemia while inhibiting the progression of oxidative stress in STZ-induced diabetic rats. Hence, it can be used in the management of diabetes mellitus. | Sherien Kamal Hassan Nermin Mohammed El-Sammad Amria Mamdouh Mousa Maha Hashim Mohammed Abd el Razik Hussein Farrag Amani Nassir Eldin Hashim Victoria Werner Ulrike Lindequist Mahmoud Abd El-Moein Nawwar | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，6： | 3 |
| 16 | Assessment of lnc RNA GAS5, lnc RNA HEIH, lnc RNA BISPR and its m RNA BST2 as serum innovative non-invasive biomarkers: Recent insights into Egyptian patients with hepatitis C virus type 4显示文摘BACKGROUND Hepatitis C virus(HCV)infection and its consequent complications are undeniably a public health burden worldwide,particularly in Egypt.Emerging evidence suggests that many lncRNAs have relevant roles in viral infections and antiviral responses.AIM To investigate the expression profiles of circulating lncRNAGAS5,lncRNAHEIH,lncRNABISPR and mRNABST2 in naïve,treated and relapsed HCV Egyptian patients,to elucidate relation to HCV infection and their efficacy as innovative biomarkers for the diagnosis and prognosis of HCV GT4.METHODS One hundred and thirty HCV-infected Egyptian patients and 20 healthy controls were included in this study.Serum lncRNAs and mRNABST2 were measured using quantitative real-time polymerase chain reaction(qRT-PCR).RESULTS Our results indicated that serum lncRNAGAS5 and LncRNABISPR were upregulated,whereas mRNA BST2 and LncRNA HEIH were downregulated in naïve patients.In contrast,HCV patients treated with sofosbuvir and simeprevir;with sofosbuvir and daclatasvir;or with sofosbuvir,daclatasvir and ribavirin exhibited lower levels of lncRNAGAS5 and lncRNABISPR with higher mRNABST2 compared to naïve patients.Notably,patients relapsed from sofosbuvir and simeprevir showed higher levels of these lncRNAs with lower mRNABST2 compared to treated patients.LncRNAGAS5 and lncRNABISPR were positively correlated with viral load and ALT at P<0.001,whereas mRNABST2 was negatively correlated with viral load at P<0.001 and ALT at P<0.05.Interestingly,a significant positive correlation between lncRNA HEIH and AFP was observed at P<0.001.CONCLUSION Differential expression of these RNAs suggests their involvement in HCV pathogenesis or antiviral response and highlights their promising roles in diagnosis and prognosis of HCV. | Nourhan M El Samaloty Marwa I Shabayek Ramy S Ghait Shohda A El-Maraghy Sherine M Rizk Maha M El-Sawalhi | 2020 | World Journal of Gastroenterology2020，26，2： | 2 |
| 17 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 18 | Study of the prevalence and association of ocular chlamydial conjunctivitis in women with genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans attending outpatient clinic显示文摘AIM: To determine the association between chlamydial conjunctivitis and genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans, in addition to the possible relationship between cultured bacterial pathogens and oculogenital chlamydial infection.METHODS: This study was performed on 100(50 symptomatic and 50 asymptomatic) women attending the Gynecological and Obstetric outpatient clinic of Alzahra hospital, Alazhar University. Simultaneously a conjunctival swab was taken from these patients.Polymerase chain reaction(PCR) was done on DNA extracted from both vaginal and conjunctival swab samples. Culture for both vaginal and conjunctival swabs was also done.RESULTS: Candida albicans was the predominant organism isolated by culture in 20% and 40% of conjunctival and vaginal swabs respectively. By the PCR method, ocular Chlamydia trachomatis was present in 60% of symptomatic women, while genital Chlamydia trachomatis infection was present in 30% of symptomatic women. The results of this method also indicated that 25/50(50%) vaginal swabs were positive with PCR for Candida albicans versus 15/50(30%) were PCR positive in conjunctival swab. Mycoplasma genitalium was present in only 10% of vaginal swabs.Concomitant oculogenital PCR positive results for Chlamydia trachomatis and Candida albicans were 30% and 28% respectively.CONCLUSION: Ocular Chlamydia trachomatis was associated with genital Chlamydia trachomatis in a high percentage of women followed by Candida albicans.Cultured bacterial organisms do not play a role in enhancement of Chlamydia trachomatis infection. | Rania Abdelmonem Khattab Maha Mohssen Abdelfattah | 2016 | International Journal of Ophthalmology(English edition)2016，9，8： | 2 |
| 19 | 反复使用抗生素患者结膜菌群的变化及耐药模式研究(英文)显示文摘目的:观察和分析反复使用抗生素患者结膜菌群的变化。此外,确定这种反复暴露对结膜菌群抵抗模式的影响。方法:研究纳入40例眼科研究所视网膜科患者。所有受试者至少进行了连续4次、每月一次的玻璃体腔注射(IVI),其中一些患者根据需要延长至6mo或1y。将患者随机分为3组,每组给予氧氟沙星、莫西沙星或头孢他啶任意一种抗生素。使用Kirby-Bauer扩散法对所有细菌进行药敏试验。结果:研究中,以表皮葡萄球菌(51.2%)为主,其次是金黄色葡萄球菌(14%),微球菌(12.8%)和其他凝固酶阴性葡萄球菌(CONS)占13%。结果显示,在4次随访中,与随机接受头孢他啶治疗的患者的基线培养相比,接受头孢他啶治疗的患者眼部表皮葡萄球菌的百分比有所增加。相比之下,与基线相比,金黄色葡萄球菌的百分比没有明显增加。在氟喹诺酮治疗眼中观察到表皮葡萄球菌的百分比从基线增加。与头孢他啶治疗组相比,氟喹诺酮治疗组的金黄色葡萄球菌百分比较基线升高。然而,随着新一代和老一代氟喹诺酮类药物的暴露,眼部菌群组成模式发生了变化。我们注意到,从基线开始,莫西沙星治疗组比氧氟沙星治疗组的表皮葡萄球菌增多。暴露于老一代和新一代氟喹诺酮类药物的金黄色葡萄球菌的模式没有差异。结论:反复使用眼用抗生素不仅会改变正常眼部菌群的组成,还会选择耐药菌株。 | Rania Abdelmonem Khattab Maha Abdelfattah Iman Fahmy | 2019 | 国际眼科杂志2019，19，11： | 2 |
| 20 | Factors Influencing the Direct Electrochemical Reduction of Nb_2O_5 Pellets to Nb Metal in Molten Chloride Salts显示文摘Powder compacted and sintered Nb_2O_5 pellets were cathodically polarised against graphite anode in calcium chloride melt at 1173 K to study the influence of various factors on the electrochemical reduction of the oxide.The parameters were;duration and temperature of electrolysis,open porosity of pellets,nature of anode,mode of electrolysis and configuration of the oxide cathode.The experiments were also conducted in KC1,KC1-25 mol%CaCl_2 and NaCl melts to understand the effect of melt composition on the electroreduction.Different Ca-Nb-O and Nb-O intermediates were found in the pellets electrolysed for different durations of time in CaCl_2 melt which eventually reduced to Nb.The current efficiency of the process decreased with increasing duration of electrolysis.Decrease in electrolysis temperature from 1173 to 1073 K led to the decrease in the rate of reduction of the oxide pellets.Pellets with high open porosity reduced faster.Carbon contamination of the melt was relatively less when pyrolytic graphite was used as anode.Of all the melts studied,the reduction was found to be better in calcium chloride melt,that too when alumina crucible was used as container of the melt. | D.Sri Maha Vishnu N.Sanil K.S.Mohandas K.Nagarajan | 2017 | Acta Metallurgica Sinica(English Letters)2017，30，3： | 2 |
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