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| 1 | Abdominal wall defects: two - versus three - dimensional uhrasonographic diagnosis 显示文摘 | Bonilla - Musoles F Machado LE Bailao LA | 2001 | J Ultrasound Med2001，20，4： | 1 |
| 2 | Upper airway cellular pattern in infants with acute bronchiolitis: neutrophils or eosinophils? 显示文摘 | Pitrez PM Pinto LA Machado DC Tsukazan MT Jones MH Stein RT | 2003 | J Pediatr (Rio J)2003，79，5： | 1 |
| 3 | The McKenzie method for low back pain : a systematic review of the literature with a meta-analysis approach 显示文摘 | Machado LA De Souza MS Ferreira PH | 2006 | Spine ( Phila Pa 1976 )2006，31，9： | 1 |
| 4 | Comparison of two rotarysystems in root canal preparation regarding disinfection显示文摘 | Machado ME Sapia LA Cai S | 2010 | J En-dod2010，36，7： | 1 |
| 5 | Impaired mitogenic responseof peripheral blood T cells in ulcerative Colitis is not due to apoptosis显示文摘 | Perez Machado MA Espinosa LM de - la - Madrigal E J | 1999 | Dig Dis Sci1999，44，： | 1 |
| 6 | Clinical evaluation of the NS1 antigen-capture ELISA for early diagnosis of dengue virus infection in Brazil显示文摘 | Castro-Jorge LA Machado PR Favero CA | 2010 | J Med Virol2010，82，8： | 1 |
| 7 | Diffusionweighted MR imaging of cystic lesions of neurocysticercrosis: a preliminary study显示文摘 | Raffin LS Baeheschi LA Machado LR | 2001 | Arq Neuropsiquiatr2001，59，4： | 1 |
| 8 | Client centered therapy vs exercise therapy for chronic low back pain: a pilot randomized cont rolled trial in Brazil显示文摘 | Machado LA Azevedo DC Capanema MB | 2007 | Pain Med2007，8，3： | 1 |
| 9 | Clinical evaluation of the NS1 antigen-capture ELISA for early di- agnosis of dengue virus infection in Brazil显示文摘 | Castro-Jorge LA Machado PR Favero CA | 2010 | J Med Vir- ol2010，82，14： | 1 |
| 10 | Diffusion-weighted MR imaging of cystic lesions of neurocysticercosis : a preliminary study显示文摘 | Raffin LS Bacheschi LA Machado LR | 2001 | Arq Neurop siquiatr2001，59，4： | 1 |
| 11 | Phototherapeutic keratectomy (PTK) and bullous keratopathy:case report显示文摘 | Obeid WN Richinho Kde P Osores AP Machado MA Obeid Rde C Vieira LA | 2005 | Arq Bras Oftalmol2005，68，5： | 1 |
| 12 | The Mckenzie method for low back pain:a systematic review of the literature with a meta-analysis approach显示文摘 | Machado LA de Souza MS Ferreira PH | 2006 | Spine(Phila Pa 1976)2006，31，9： | 1 |
| 13 | Effect of disinfectants on hardness and roughness of reline acrylic resins显示文摘 | Azevedo A Machado LA Vargani CE | 2006 | J Prosthodont2006，15，4： | 1 |
| 14 | Client-centered therapy vs exercise therapy for chronic low back pain:a pilot randomized controlled trial in Brazil显示文摘 | Machado LA Azevedo DC Capanema MB | 2007 | Pain Med2007，8，3： | 1 |
| 15 | The McKenzie Method for Low Back Pain A Systematic Review of the Literature With a meta-analysis approach 显示文摘 | Machado LA de Souza MS Ferreira PH | 2006 | Spine2006，31，9： | 1 |
| 16 | Optimized procedure for DNA isolation from fresh and cryopreserved clotted human blood useful in clinical molecular testing显示文摘 | Salazar LA Hirata MH Cavalli SA Machado MO Hirata RDC | 1998 | Clin Chem1998，44，： | 1 |
| 17 | The effect of age on the monoamines of the hypothalamus 显示文摘 | Rodriguez GJA de la Roza C Machado A | 1995 | Mech Ageing Dev1995，77，： | 1 |
| 18 | Client-cen- tered therapy vs exercise therapy for chronic low back pain: a pilot randomized controlled trial in Brazil 显示文摘 | Machado LA Azevedo DC Capanema MB | 2007 | Pain Med2007，8，3： | 1 |
| 19 | In vitro induced pluripotency from urine-derived cells in porcine显示文摘BACKGROUND The generation of induced pluripotent stem cells(iPSC)has been a game-changer in translational and regenerative medicine;however,their large-scale applicability is still hampered by the scarcity of accessible,safe,and reproducible protocols.The porcine model is a large biomedical model that enables translational applications,including gene editing,long term in vivo and offspring analysis;therefore,suitable for both medicine and animal production.AIM To reprogramme in vitro into pluripotency,and herein urine-derived cells(UDCs)were isolated from porcine urine.METHODS The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4(OCT4),SRY-box2(SOX2),Kruppel-like factor 4(KLF4),and C-MYC,and cultured with basic fibroblast growth factor(bFGF)supplementation.To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase(AP),OCT4,SOX2,NANOG,TRA181 and SSEA 1 detection.Endogenous transcripts related to the pluripotency(OCT4,SOX2 and NANOG)were analyzed via reverse transcription quantitative realtime polymerase chain reaction in different time points during the culture,and all three lineages formed embryoid bodies(EBs)when cultured in suspension without bFGF supplementation.RESULTS The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming.Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors(mOSKM),but not from human factors(hOSKM).Three clonal lineages were isolated and further cultured for at least 28 passages,all the lineages were positive for AP detection,the OCT4,SOX2,NANOG markers,albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1,similar results were observed in the abundance of the endogenous transcripts related to pluripotent state.All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts.CONCLUSION For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotentlike state,enabling new numerous applications in both human or veterinary regenerative medicine. | Kaiana Recchia Lucas Simões Machado Ramon Cesar Botigelli Naira Caroline Godoy Pieri Gabriela Barbosa Raquel Vasconcelos Guimarães de Castro Mariana Groke Marques Laís Vicari de Figueiredo Pessôa Paulo Fantinato Neto Flávio Vieira Meirelles Aline Fernanda de Souza Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan | 2022 | World Journal of Stem Cells2022，14，3： | 0 |
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