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| 1 | Microbiota modification by probiotic supplementation reduces colitis associated colon cancer in mice显示文摘AIM To investigate the effect of probiotic supplementation during the development of an experimental model of colitis associated colon cancer(CAC). METHODS C57 BL/6 mice received an intraperitoneal injection of azoxymethane(10 mg/kg), followed by three cycles of sodium dextran sulphate diluted in water(5% w/v). Probiotic group received daily a mixture of Lactobacillus acidophilus, Lactobacil us rhamnosus and Bifidobacterium bifidum. Microbiota composition was assessed by 16 Sr RNA Illumina Hi Seq sequencing. Colon samples were collected for histological analysis. Tumor cytokines was assessed by Real Time-PCR(Polymerase Chain Reaction); and serum cytokines by Multiplex assay. All tests were two-sided. The level of significance was set at P < 0.05. Graphs were generated and statistical analysis performed using the software Graph Pad Prism 5.0. The project was approved by the institutional review board committee. RESULTS At day 60 after azoxymethane injection, the mean number of tumours in the probiotic group was 40% lower than that in the control group, and the probiotic group exhibited tumours of smaller size(< 2 mm)(P < 0.05). There was no difference in richness and diversity between groups. However, there was a significant difference in beta diversity in the multidimensional scaling analysis. The abundance of the genera Lactobacillus, Bifidobacterium, Allobaculum, Clostridium XI and Clostridium XVⅢ increased in the probiotic group(P < 0.05). The microbial change was accompanied by reduced colitis, demonstrated by a 46% reduction in the colon inflammatory index; reduced expression of the serum chemokines RANTES and Eotaxin; decreased p-IKK and TNF-α and increased IL-10 expression in the colon. CONCLUSION Our results suggest a potential chemopreventive effect of probiotic on CAC. Probiotic supplementation changes microbiota structure and regulates the inflammatory response, reducing colitis and preventing CAC. | Maria Carolina S Mendes Daiane SM Paulino Sandra R Brambilla Juliana A Camargo Gabriela F Persinoti José Barreto C Carvalheira | 2018 | World Journal of Gastroenterology2018，24，18： | 17 |
| 2 | Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells显示文摘AIM:To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells. METHODS: Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths. RESULTS: Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P < 0.05) adhesion to all the cell lines and clones tested, when compared to the non-pathogenic strain HPTx30a. Bacteria showed a significantly higher (P < 0.05) adhesion to the GP202 cell line, when compared to the MKN45 cell line. Furthermore, both strains showed a significantly higher (P < 0.05) adhesion to GP202 clones with larger MUC1 VNTR domains. CONCLUSION: This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells. The extent of bacterial adhesion depends on the size of theMUC1 VNTR domain. The adhesion is further dependent on bacterial pathogenicity and the gastric cell line. MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa. | Natália R Costa Nuno Mendes Nuno T Marcos Celso A Reis Thomas Caffrey Michael A Hollingsworth Filipe Santos-Silva | 2008 | World Journal of Gastroenterology2008，14，9： | 4 |
| 3 | Trends in overall opportunistic illnesses, Pneumocystis carinii pneumonia, cerebral toxoplasmosis and Mycobacterium avium complex incidence rates over the 30 years of the HIV epidemic: a systematic review显示文摘 | Lara Coelho Valdiléa Gon?alves Veloso Beatriz Grinsztejn Paula Mendes Luz | 2013 | Brazilian Journal of Infectious Diseases2013，，： | 2 |
| 4 | Phenylalanine hydroxylase deficiency:Molecular epidemiology and predictable BH (4)-responsiveness in South Portugal PKU patients显示文摘 | Rivera I Mendes D Afonso A | 2011 | Mol Genet Metab2011，104，1： | 1 |
| 5 | An enzyme-linked immunosorbent assay for the detection of vesicular stomatitis virus antibodies 显示文摘 | Allende R Sepulveda L Mendes da Silva A | 1992 | Prev Vet Med1992，14，： | 1 |
| 6 | Scanning electron microsco- py evaluation of the bonding mechanism of a self - etching primer on enamel显示文摘 | Cal -Neto J P Mendes -Miguel J A | 2006 | Angle Orthod2006，76，1： | 1 |
| 7 | The role of constraints within generalized nonextensive statistics显示文摘 | Tsallis C Mendes R S Plastino A R | 1998 | Physica A:Statistical Mechanics and its Applications1998，261，3： | 1 |
| 8 | One dimensional model of phase holdups in three phase stratified flow显示文摘 | Khor S H Mendes Tatsis M A Hewitt G F | 1997 | Int J Muhiphase Flow1997，23，5： | 1 |
| 9 | Coverage of the left subclavian artery during thoracic endovascular aortic repair 显示文摘 | Riesenman P J Farber M A Mendes R R | 2007 | J Vasc Surg2007，45，1： | 1 |
| 10 | Crypthecodinium cohnii with emphasis on DHA production:a review显示文摘 | Mendes A Reis A Vasconcelos R | 2009 | J Appl Phycol2009，21，2： | 1 |
| 11 | Handgrip strength at ad- mission and time to discharge in medical and surgical inpatients 显示文摘 | Mendes J Azevedo A Amaral T F | 2013 | Journal of Parenteral and Enteral Nutrition2013，，： | 1 |
| 12 | Familial hyperc- holesterolemia: Molecular characterization of possible cases from the Azores Islands (Portugal) 显示文摘 | Cymbron T Mendes P Ramos A | 2014 | Meta Gene2014，14，2： | 1 |
| 13 | Functional transcranial Doppler: presymptomatic changes in Fabry disease显示文摘 | Azevedo E Mendes A Seixas 0 | 2012 | Eur Neurol2012，67，6： | 1 |
| 14 | The effect of supplementation with expanded sunflower seed on carcass and meat quality of lambs raised on pasture 显示文摘 | Santos Silva J Bessa R J B Mendes I A | 2003 | Meat Science2003，65，： | 1 |
| 15 | Theinfluence of doublepulse on porosity formation in aluminum GMAW 显示文摘 | Mendes da Silva C L Scotti A | 2006 | Journal of Materials Processing Technology2006，171，： | 1 |
| 16 | Ectopic micronodular thymoma with lymphoid stroma显示文摘 | Mende S Moschopulos M Marx A | 2004 | Virchows Arch2004，444，4： | 1 |
| 17 | Thermal actions for concrete bridge design 显示文摘 | Branco F A Mendes P A | 1993 | Journal of Structural Engineering1993，119，8： | 1 |
| 18 | Long-term progressive deterioration following fire exposure of OPC versus slag blended cement pastes 显示文摘 | Mendes A Sanjayan J G Frank | 2009 | Materials and Structures2009，42，1： | 1 |
| 19 | Numerical simulation of reactive transfers in spouted beds at high temperature:application to coal gasification显示文摘 | Mendes A Dollet A Ablitzer C | 2008 | Journal of Analytical and Applied Pyrolysis2008，82，1： | 1 |
| 20 | Comparison of practical methods for urinary glycosaminoglycans and serum hyaluronan w ith clinical activity scores in patients w ith Grave’s ophthalmopathy显示文摘 | Martins JR Furlanetto RP Oliveira LM Mendes A Passerotti CC Chiamolera MI | 2004 | Clin Endocrinol2004，60，6： | 1 |
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