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| 1 | 肺炎链球菌呼吸道感染分离株的耐药性显示文摘目的 :研究肺炎链球菌呼吸道感染分离株的耐药性及其传播情况。方法 :肺炎链球菌分离株进行药物敏感试验和血清学分型 ,并结合BOXPCR、脉冲场电泳 (pulse-fieldgelelectrophoresis,PFGE)和青霉素结合蛋白基因 (penicillinbindingpro teingene,pbp)指纹等分子生物学方法分析菌株间亲缘关系。 结果 :呼吸道感染分离株 1 2 8株中有青霉素低度耐药株 (peni cillinintermediatedStreptococcuspneumoniae,PISP) 1 0株 ,未发现青霉素高度耐药株 (penicillinresistantStreptococcuspneumoni ae,PRSP)。 1 1 1株存活菌株分属 2 5种血清型 ,主要为 2 3F、3、1 9F、1 4、9V、6A、6B等。 9株存活PISP中有 6种PFGE谱型 ,2株PFGE谱型相同菌株的耐药谱和血清型相仿 ;2株不同PFGE谱型菌株pbp指纹相似 ,对青霉素和头孢曲松敏感性相似 ;上述呼吸道感染分离PISP中未发现与世界主要流行耐药克隆代表株DNA指纹或 pbp基因相似菌株。 结论 :上述肺炎链球菌呼吸道感染分离株青霉素耐药率尚低 ,存在青霉素耐药克隆和基因的传播 ,其中尚未发现世界主要流行耐药克隆。 | 杨帆 张婴元 Lesley McGee Avril Wasas Keith Klugman 吴卫红 周乐 朱德妹 汪复 | 2001 | 中国抗感染化疗杂志2001，1，1： | 26 |
| 2 | AMPK调节骨骼肌细胞GLUT4基因表达的机制研究显示文摘腺苷酸活化蛋白激酶(AMPK)能调节运动/肌肉收缩所引起的骨骼肌细胞葡萄糖转运蛋白4(GLUT4)基因的表达,但至今它的调节机制不清。研究显示在非运动刺激引起的细胞信号事件中由组蛋白去乙酰化酶(HDACs)以及组蛋白乙酰化酶(HATs)控制的组蛋白乙酰化状态是调节基因表达的重要机制,所以我们假设AMPK信号途径是通过征用HDACs中的HDAC5(在骨骼肌细胞内高表达)来实现对运动/肌肉收缩引起的GLUT4基因表达控制。细胞分为正常浓度葡萄糖对照组(NGLU组)、正常浓度AICAR组(NGLU+AICAR组)、高浓度对照组(HGLU组)、高浓度AICAR组(HGLU+AICAR组)。用5 mmol/L和20 mmol/L葡萄糖浓度培养骨骼肌细胞后,NGLU+AICAR组和HGLU+AICAR组与肌肉收缩模拟信号刺激5-氨基-4-甲酰胺咪唑核糖核苷酸(AICAR)孵育。AICAR能激活NGLU组骨骼肌细胞AMPKα2、减少骨骼肌细胞核HDAC5蛋白、促使HDAC5与骨骼肌细胞加强因子(MEF2)蛋白分离和上调GLUT4基因的表达;相反,高浓度葡萄糖延迟由AICAR引起的AMPKα2磷酸化、AMPKα2向细胞核转入、HDAC5向细胞核转出和GLUT4基因的表达。实验结果说明在不同葡萄糖浓度下的骨骼肌细胞GLUT4基因表达变化都对应着上游AMPK蛋白和下游HDAC5蛋白的变化,AMPK可能是征用转录抑制子HDAC5来调节MEF2的活性而达到控制肌肉收缩所引起的GLUT4基因表达。 | 李良刚 陈槐卿 Sean L. McGee | 2008 | 生物医学工程学杂志2008，25，1： | 7 |
| 3 | LIMS实施经验之谈显示文摘实验室必须花费必要的时间和资源去规划、选择并实施一个新的LIMS系统。 | George Avery Charles McGee Stan Falk 闫蕊 梁斌 | 2004 | 现代科学仪器2004，21，5： | 7 |
| 4 | TDP-43 regulates cancer-associated microRNAs显示文摘 | Xiaowei Chen Zhen Fan Warren McGee Mengmeng Chen Ruirui Kong Pushuai Wen Tengfei Xiao Xiaomin Chen Jianghong Liu Li Zhu Runsheng Chen Jane Y. Wu | 2018 | Protein & Cell2018，9，10： | 4 |
| 5 | 上海市791名健康儿童222株肺炎链球菌携带株研究显示文摘目的 了解健康儿童肺炎链球菌携带株的流行病学特征。方法 测定来自上海 5所幼儿园 (代号分别为JR、PL、SY、WS、ZF) 791名健康儿童鼻咽部分离的 2 2 2株肺炎链球菌健康儿童携带株的耐药谱和血清型 ,并以PFGE、BOXPCR和pbp基因指纹等分子生物学方法分析菌株间亲缘关系。结果 2 2 2株携带株中有青霉素低度耐药株 32株 ,未发现青霉素高度耐药株。全部菌株包括 2 2种血清型 ,常见为 2 3F(2 5 7% )、6A(13 1% )、19F(10 8% )、6B(8 1% )等 ;青霉素低度耐药株包括 8种血清型 ,主要为 2 3F(5 0 % )、19A和 19F(各占 15 6 % )。 32株青霉素低度耐药菌株可分为 8种BOXPCR谱型和 7种PFGE谱型。PFGE谱型A菌株的耐药谱、血清型以及BOXPCR和pbp基因指纹高度一致。自幼儿园SY的儿童分离的肺炎链球菌青霉素耐药率 31 8% ,远高于平均水平 ,该园 2 1株青霉素耐药菌株中 2 0株属于PFGE谱型A或B。结论 上海 5所幼儿园健康儿童肺炎链球菌携带株青霉素耐药率总体尚较低 ,但个别幼儿园具有较高耐药率 。 | 杨帆 张婴元 Lesley McGee Avril Wasas Keith Klugman 吴卫红 周乐 朱德妹 汪复 | 2001 | 中华医学杂志2001，81，10： | 3 |
| 6 | 腺苷酸活化蛋白激酶与运动研究进展显示文摘运动能刺激骨骼肌对葡萄糖摄取 ,提高对胰岛素的敏感性 ,促进脂肪酸的氧化等 ,即使是一次训练课就能够引起许多代谢基因显著变化。腺苷酸活化蛋白激酶 (5’ -AMP -activatedpro teinkinase ,AMPK)作为能量代谢变化的感受器能够被运动中ATP/AMP的比值变化所激活 ,在调节这些对健康有利的代谢适应过程中起着十分重要的作用。许多研究表明AMPK与运动关系密切 :一方面运动能够对AMPK的活性、磷酸化以及基因和蛋白表达等产生多种影响 ;另一方面AMPK自身的变化 ,特别是一些重要位点的突变也会对运动能力产生深刻影响。本文总结了AMPK与运动相互影响的最新研究成果。 | 李良刚 Mark Hargreaves Sean McGee | 2005 | 中国运动医学杂志2005，24，2： | 3 |
| 7 | Helicobacter pylori arginase mutant colonizes arginase Ⅱ knockout mice显示文摘AIM: To investigate the role of host and bacterial arginases in the colonization of mice by Helicobacter pylori (H.pylori).METHODS: H.pylori produces a very powerful urease that hydrolyzes urea to carbon dioxide and ammonium,which neutralizes acid.Urease is absolutely essential to H.pylori pathogenesis;therefore,the urea substrate must be in ample supply for urease to work efficiently.The urea substrate is most likely provided by arginase activity,which hydrolyzes L-arginine to L-ornithine and urea.Previous work has demonstrated that H.pylori arginase is surprisingly not required for colonization of wild-type mice.Hence,another in vivo source of the critical urea substrate must exist.We hypothesized that the urea source was provided by host arginase Ⅱ,since this enzyme is expressed in the stomach,and H.pylori has previously been shown to induce the expression of murine gastric arginase Ⅱ.To test this hypothesis,wild-type and arginase (rocF) mutant H.pylori strain SS1 were inoculated into arginase Ⅱ knockout mice.RESULTS: Surprisingly,both the wild-type and rocF mutant bacteria still colonized arginase Ⅱ knockout mice.Moreover,feeding arginase Ⅱ knockout mice the host arginase inhibitor S-(2-boronoethyl)L-cysteine (BEC),while inhibiting > 50% of the host arginase Ⅰ?activity in several tissues,did not block the ability of the rocF mutant H.pylori to colonize.In contrast,BEC poorly inhibited H.pylori arginase activity.CONCLUSION: The in vivo source for the essential urea utilized by H.pylori urease is neither bacterial arginase nor host arginase Ⅱ;instead,either residual host arginase Ⅰ?or agmatinase is probably responsible. | Songhee H Kim Melanie L Langford Jean-Luc Boucher Traci L Testerman David J McGee | 2011 | World Journal of Gastroenterology2011，17，28： | 3 |
| 8 | Effect of interface on mid-infrared photothermal response of MoS2 thin film grown by pulsed laser deposition显示文摘这研究报导在上中间红外线(中间 -- 红外) 多层的瞬间 2 的 photothermal 反应在上成年的薄电影水晶(p 类型硅和 c-axis-oriented 单身者水晶蓝宝石) 并且非结晶(Si/SiO 2 和 Si/SiN ) 由搏动的激光免职(PLD ) 的底层。当照耀与时,瞬间 2 电影的 photothermal 反应在瞬间 2 电影的抵抗作为变化被测量一中间 -- 红外(7 ~ 8.2 m ) 来源。我们显示出那提高瞬间 2 的抵抗(TCR ) 的温度系数薄电影由通过底层和生长条件的一种合适的选择控制电影底层接口是可能的。PLD 种的薄电影用 X 光检查衍射,拉曼,原子力量显微镜学, X 光检查光电子显微镜学,和传播电子被描绘显微镜学。高分辨率的传播电子显微镜学(HRTEM ) 图象证明瞬间 2 电影与不合身的衣服脱臼以一种 layer-by-layer 方式在蓝宝石底层上成长。当这些电影在有一颗钻石的底层上是成年的时,层生长形态学被破坏立方的结构(例如,硅) 因为成双的生长形成。非结晶的底层上的生长形态学例如 Si/SiO 2 或 Si/SiN,是很不同的。硅上的 PLD-grown 瞬间 2 电影显示出更高的 TCR (在 296 K 的 2.9% K 1) ,更高中间 -- 红外敏感(R/R =5.2%) ,并且更高的 responsivity (8.7 V 湡杯湥牥瑡牯吗?? | Ankur Goswami Priyesh Dhandaria Soupitak Pal Ryan McGee Faheem Khan Zeljka Antic Ravi Gaikwad Kovur Prashanthi Thomas Thundat | 2017 | Nano Research2017，10，10： | 2 |
| 9 | Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle显示文摘Background: Feed accounts for up to 75% of costs in beef production systems,thus any improvement in feed efficiency(FE) will benefit the profitability of this enterprise.Residual feed intake(RFI) is a measure of FE that is independent of level of production.Adipose tissue(AT) is a major endocrine organ and the primary metabolic energy reservoir.It modulates a variety of processes related to FE such as lipid metabolism and glucose homeostasis and thus measures of inter-animal variation in adiposity are frequently included in the calculation of the RFI index.The aim of this study was to determine the effect of phenotypic RFI status and gender on the expression of key candidate genes related to processes involved in energy metabolism within AT.Dry matter intake(DMI) and average daily gain(ADG) were measured over a period of 70 d for 52 purebred Simmental heifers(n = 24) and bulls(n = 28) with an initial BW±SD of 372±39.6 kg and 387±50.6 kg,respectively.Residual feed intake was calculated and animals were ranked within gender by RFI into high(inefficient; n = 9 heifers and n = 8 bulls)and low(efficient; n = 9 heifers and n = 8 bulls) groups.Results: Average daily gain ±SD and daily DMI ±SD for heifers and bul s were 1.2±0.4 kg and 9.1±0.5 kg,and 1.8±0.3 kg and 9.5±1 kg respectively.High RFI heifers and bulls consumed 10% and 15% more(P < 0.05) than their low RFI counterparts,respectively.Heifers had a higher expression of all genes measured than bulls(P < 0.05).A gender × RFI interaction was detected for HMGCS2(P < 0.05) in which high RFI bulls tended to have lower expression of HMGCS2 than low RFI bulls(P < 0.1),whereas high RFI heifers had higher expression than low RFI heifers(P < 0.05) and high RFI bulls(P < 0.05).SLC2 A4 expression was consistently higher in subcutaneous AT of low RFI animals across gender.Conclusion: The findings of this study indicate that low RFI cattle exhibit upregulation of the molecular mechanisms governing glucose metabolism in adipose tissue,in particular,glucose clearance.The decreased expression of SLC2 A4 in the inefficient cattle may result in less efficient glucose metabolism in these animals.We conclude that SLC2 A4 may be a potential biomarker for RFI in cattle. | Clare McKenna Richard K.Porter Kate A.Keogh Sinead M.Waters Mark McGee David A.Kenny | 2019 | Journal of Animal Science and Biotechnology2019，10，1： | 2 |
| 10 | X-linked retinitis pigmentosa: mutation spectrum of the RPGR and RP2 genes and correlation with visual function显示文摘 | Sharon D Bruns GAP McGee TL Sandberg MA Berson EL Dryja TP | 2000 | Invest Ophthalmol Vis Sci2000，41，9： | 1 |
| 11 | Involvement of exodermal passage cells in mycorrhizal infection of some orchids显示文摘 | Esnault A L Masuhara G McGee R A | 1994 | Mycol Res1994，98，6： | 1 |
| 12 | A point mutation of the rhodopsin gene in one form of retinitis pigmentosa 显示文摘 | Dryja TP McGee TL Reichel E | 1990 | Nature1990，343，： | 1 |
| 13 | Glycine max: a potential hostof the peanut web blotch fungus显示文摘 | Smith D H McGee R E | 1981 | Proceedings of theAmerican Peanut Research and Education Society1981，，13： | 1 |
| 14 | Pure natural orifice transluminal endoscopic surgery (NOTES) nephrectomy using standard laparoscopic in- struments in the porcine model显示文摘 | Isariyawongse J P McGee M F Rosen M J | 2008 | J Endourol2008，22，： | 1 |
| 15 | Jirstromexpression posi-tively ct)iTelates with poor prognosis in breast cancer patients显示文摘 | McSherry EA McGee SF | 2009 | lnt JCancer2009，125，6： | 1 |
| 16 | Preventing complications of central venouscatheterization显示文摘 | McGee DCjGould MK | 2003 | N Eng IJ MED2003，34，8： | 1 |
| 17 | Making up Mind:The Early Sociology of Invention 显示文摘 | David McGee | 1995 | Technology and Culture1995，36，4： | 1 |
| 18 | Prevention of perinatal group B streptococcal disease--revised guidelines from CDC,2010显示文摘 | Verani JR McGee L SchragSJ | 2010 | MMWR Recomm Rep2010，59，10： | 1 |
| 19 | AMP-activated protein kinase regulates GLUT4 transcription by phosphorylating histone deacetylase 5显示文摘 | MCGEE S L VANDENDEREN B J HOWLETT K F | | 0，，04： | 1 |
| 20 | Preventing complications of central venous catheterization 显示文摘 | Mcgee DC Gould MK | 2003 | N Engl J Med2003，348，12： | 1 |
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