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| 1 | Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4显示文摘Objective:To investigate the cytotoxic activity of actinomycete isolated from marine sediment.Methods:In the present study the DNA was isolated and the ITS region of 16s rRNA was amplified by polymerase chain reaction,using two universal bacterial primers,1492K(5'-GGTTACCTTG'TTAC GACTT-3')and Eubac27F(5'-AGAGTTTGATCCTGGCTC AG-3').The amplified products were purified using TIANgel mini purification kit,ligated to MD18-T simple vector(TaKaRa),and transformed into competent cells of Escherichia coli DH5α.16S rRNA gene fragment was sequenced using forward primer M13F(-47)and reverse primer M13R(-48).Blast search sequence similarity was found against the existing non-redundanl nucleotide sequence database thus,identified as Streptomyces sp SU,Streptomyces rubralavandulae strain SU1,Streptomyces cacaoi strain SU2,Streptomyces cavourensis strain SU3,Streptomyces avidinii strain SU4,Streptomyces globisporus strain SU5,Streptomyces variabilis strain SU6,Streptomyces coelicolor strain SU 7.Among the eight identified isolates,one actinomycete Streptomyces avidinii strain SU4 was selected for further study.Results:Crude extract of the actinomycete isolate exhibited IC_(50)in 64.5μg against Hep-2 cell line,250μg in VERO cell line.This value is very close to the criteria of cytotoxicity activity for the crude extracts,as established by the American National Cancer Institute(NCI)is in IC_(50)<30μg/mL.The CC MS analysis showed that the active principle might be 1,2-benzenedicarboxylic acid,bis(2-methylpropyl)ester(12.17%),isooctyl phthalate(15.29%)with the retention time 15.642 and 21.612,respectively.Conclusions:This study clearly proves that the marine sediment derived actinomycetes with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical and anticancer screening programs.These results help us to conclude that the potential of using metabolic engineering and post genomic approaches to isolate more bioactive compounds and make their possible commercial application is not far off. | Sudha S Masilamani Selvam M | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，10： | 2 |
| 2 | B cell activation leads to shedding of complement receptor type Ⅱ (CR2/CD21)显示文摘 | Masilamani M Kassahn D Mikkat S | 2003 | Eur J Immunol2003，33，9： | 1 |
| 3 | Variation in stress-nclucecl antioxiclant enzyme activities among threemulberry euhivars显示文摘 | Chaitanya K V Sundar I) Masilamani S | 2002 | Plant Growth Regulation2002，36，： | 1 |
| 4 | Escitalopram(Lexapro) for depression显示文摘 | Masilamani S Ruppelt S C | 2003 | Am Fam Physician2003，68，11： | 1 |
| 5 | Variation in heat stress-induced antioxidant enzyme activities among three mul- berry cultivars 显示文摘 | Chaitanya K V Sundar D Masilamani S | 2002 | Plant Growth Regulation2002，36，2： | 1 |
| 6 | Regulation of collecting duct AQP3 expression:response to mineralocorticoid显示文摘 | Kwon TH Nielsen J Masilamani S | | 0，，06： | 1 |
| 7 | Microbial transformation of zaluzanin-D显示文摘 | Kumari G N K Masilamani S Ganesh M R | 2003 | Phytochemistry2003，62，： | 1 |
| 8 | Variation in heat stress-induced antioxidant enzyme activities among three mulberry cultivars 显示文摘 | Chaitanya K V Sundar D Masilamani S | 2002 | Plant Growth Regulation2002，36，2： | 1 |
| 9 | The renal thiazide-sensitive Na-C1 cotransportor of the aldosterone-escape plenomenon显示文摘 | Wang XY Masilamani S Nielsen J | 2001 | J Clin Invest2001，108，： | 1 |
| 10 | The renal thiazide-sensitive Na-Cl contransportet as mediator of the aldosterone-escape phenomenon显示文摘 | Wang XY Masilamani S Nielsen J | 2001 | J Clin Invest2001，108，2： | 1 |
| 11 | The lipid raft microdomain-associated protein reggie-1/flotillin-2 is expressed in human B cells and localized at the plasma membrane and centrosome in PBMCs显示文摘 | Solomon S Masilamani M Rajendran L | 2002 | Immunobiology2002，205，1： | 1 |
| 12 | Regulation of collecting duct AQP3 expression:response to mineralocorticoid显示文摘 | Nielsen J Masilamani s | 2002 | Am J Physiol Renal Physiol2002，283，： | 1 |
| 13 | Regulation of col-lecting duct AQP3 expression:response to mineralocorticoid显示文摘 | Kwon TH Nielsen J Masilamani S | 2002 | Am J Physiol Renal Physiol2002，283，6： | 1 |
| 14 | Escitalopram (Lexapro) for depression显示文摘 | Ruppelt SC | 2003 | Am Fam Physician2003，68，11： | 1 |
| 15 | Asymmetric localization of flotillins/reggies in preassembled platforms confers inherent polarity to hematopoietic ceils 显示文摘 | Rajendran L Masilamani M Solomon S | 2003 | PNAS2003，100，14： | 1 |
| 16 | Regulation of collecting duct AQP3 expression:response to mineralocorticoid显示文摘 | Kwon TH Nielsen J Masilamani S | 2002 | Am J Physiol Renal Physiol2002，283，6： | 1 |
| 17 | Asymmetric localization of flotillins/reggies in preassembled platforms confers inherent polarity to hematopoietic cells显示文摘 | RAJENDRAN L MASILAMANI M SOLOMON S | 2003 | Proc Natl Acad Sci USA2003，100，14： | 1 |
| 18 | Experimental investigation to study the influence of handle diameter on low-frequency, hand-arm vertical vibration 显示文摘 | MASILAMANY S A KRISHNASWAMY S SUTHANGATHAN P S | 2011 | Human Factors and Ergonomics in Manufacturing & Service Industries2011，23，2： | 1 |
| 19 | Cloning, expression of b-l, 341, 4 glucanase from Bacillus subtilis SU40 and the effect of calcium ion on the stability of re- combinant enzyme: in vitro and in silico analysis 显示文摘 | Masilamani R Sharma O P Muthuvel S K | 2013 | Bioin- formation2013，19，9： | 1 |
| 20 | Antifungal Fractions and Compounds from Uncrushed Green Leaves of Azadirachta indica 显示文摘 | Suresh G Narasinhan N S Masilamani S | 1997 | Phytoparasitica1997，25，： | 1 |
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