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| 1 | Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4显示文摘Objective:To investigate the cytotoxic activity of actinomycete isolated from marine sediment.Methods:In the present study the DNA was isolated and the ITS region of 16s rRNA was amplified by polymerase chain reaction,using two universal bacterial primers,1492K(5'-GGTTACCTTG'TTAC GACTT-3')and Eubac27F(5'-AGAGTTTGATCCTGGCTC AG-3').The amplified products were purified using TIANgel mini purification kit,ligated to MD18-T simple vector(TaKaRa),and transformed into competent cells of Escherichia coli DH5α.16S rRNA gene fragment was sequenced using forward primer M13F(-47)and reverse primer M13R(-48).Blast search sequence similarity was found against the existing non-redundanl nucleotide sequence database thus,identified as Streptomyces sp SU,Streptomyces rubralavandulae strain SU1,Streptomyces cacaoi strain SU2,Streptomyces cavourensis strain SU3,Streptomyces avidinii strain SU4,Streptomyces globisporus strain SU5,Streptomyces variabilis strain SU6,Streptomyces coelicolor strain SU 7.Among the eight identified isolates,one actinomycete Streptomyces avidinii strain SU4 was selected for further study.Results:Crude extract of the actinomycete isolate exhibited IC_(50)in 64.5μg against Hep-2 cell line,250μg in VERO cell line.This value is very close to the criteria of cytotoxicity activity for the crude extracts,as established by the American National Cancer Institute(NCI)is in IC_(50)<30μg/mL.The CC MS analysis showed that the active principle might be 1,2-benzenedicarboxylic acid,bis(2-methylpropyl)ester(12.17%),isooctyl phthalate(15.29%)with the retention time 15.642 and 21.612,respectively.Conclusions:This study clearly proves that the marine sediment derived actinomycetes with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical and anticancer screening programs.These results help us to conclude that the potential of using metabolic engineering and post genomic approaches to isolate more bioactive compounds and make their possible commercial application is not far off. | Sudha S Masilamani Selvam M | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，10： | 2 |
| 2 | B cell activation leads to shedding of complement receptor type Ⅱ (CR2/CD21)显示文摘 | Masilamani M Kassahn D Mikkat S | 2003 | Eur J Immunol2003，33，9： | 1 |
| 3 | The cell biology of the human natural killer cell CD94/NKG2A inhibitory receptor显示文摘 | Borrego F Masilamani M Kabat J | 2005 | Mol Immunol2005，42，4： | 1 |
| 4 | The inhibitory leukocyte-associated Ig-like receptor-1 (LAIR-1) is expressed at high level by human naive T cells and inhibit TCR mediated activation显示文摘 | Maassho K Masilamani M Valas R | 2005 | Mol Immunol2005，42，12： | 1 |
| 5 | The inhibitory LAIR-1 is expressed at high levels by human naive T cells and inhibits TCR mediated activation 显示文摘 | Maasho K Masilamani M Valas R | 2005 | Mol Immunol2005，42，12： | 1 |
| 6 | The inhibitory leukocyte associated Ig like receptor-1 (LAIR-l) is expressed at high levels by human naive T ceils and inhibits TCR mediated activation显示文摘 | Maasho K Masilamani M Valas R | 2005 | Mol Immunol2005，42，12： | 1 |
| 7 | Decreased levels of serum soluble complement receptor-II (CR2/CD21) in patients with rheumatoid arthritis显示文摘 | Masilamani M von Kempis J Illges H | 2004 | Rheumatology2004，43，2： | 1 |
| 8 | CD94/NKG2A inhibits NK cell activation by disrupting the actin network at the immunological synapse显示文摘 | Masilamani M Nguyen C Kabat J et a | 2006 | J Immunol2006，177，： | 1 |
| 9 | Cancer diagaaosis by autofluorescence of blood components显示文摘 | Masilamani V AI-Zhrani K AI-Salhi M | 2004 | J Luminescence2004，109，34： | 1 |
| 10 | Microbial transformation of zaluzanin-D显示文摘 | Kumari G N K Masilamani S Ganesh M R | 2003 | Phytochemistry2003，62，： | 1 |
| 11 | 显示文摘 | Borrego F Masilamani M Kabat J | 2005 | Mol Immunol2005，42，4： | 1 |
| 12 | Cancer diagnosis by autofluorescence of blood components显示文摘 | V Masilamani K Al-Zhrani M Al-Salhi | 2004 | Journal of Luminescence2004，109，： | 1 |
| 13 | The inhibitory leukocyte- associated Ig-like receptor-1 (LAIR-I) is expressed at high levels by human naive T cells and inhibits TCR mediated activation显示文摘 | Maasho K Masilamani M Valas R | 2005 | Molecular Immunology2005，42，12： | 1 |
| 14 | CD94/NKG2A inhibits NK cell activation by disrupting the actin network at the immunological synapse显示文摘 | Masilamani M Nguyen C Kabat J | | 0，，06： | 1 |
| 15 | The cell bilogy of the human natural killer cell CD94 /NKG2A inhibitory receptor 显示文摘 | Borrego F Masilamani M Kabat J | 2005 | Mol Immunol2005，42，4： | 1 |
| 16 | The lipid raft microdomain-associated protein reggie-1/flotillin-2 is expressed in human B cells and localized at the plasma membrane and centrosome in PBMCs显示文摘 | Solomon S Masilamani M Rajendran L | 2002 | Immunobiology2002，205，1： | 1 |
| 17 | The NKG2D receptor:immunobiology and clinical implications显示文摘 | Burgess SJ Maasho K Masilamani M | 2008 | Immunol Res2008，40，1： | 1 |
| 18 | Regnlation of the immune response by soybean isoflavones 显示文摘 | Masilamani M Wei J Sampson HA | 2012 | Immunol Res2012，54，13： | 1 |
| 19 | CD94/NKG2A inhibits NK cell activation by disrupting the aetin network at the immunological synapse 显示文摘 | Masilamani M Nguyen C Kabat J | 2006 | J Immunol2006，177，6： | 1 |
| 20 | The NKG2 D receptor : immunobiology and clinical implications 显示文摘 | Burgess SJ Maasho K Masilamani M | 2008 | Immunol Rcs2008，40，1： | 1 |
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