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| 1 | Estimating the basic reproductive ratio for the Ebola outbreak in Liberia and Sierra Leone显示文摘Background:Ebola virus disease has reemerged as a major public health crisis in Africa,with isolated cases also observed globally,during the current outbreak.Methods:To estimate the basic reproductive ratio R0,which is a measure of the severity of the outbreak,we developed a SEIR(susceptible-exposed-infected-recovered)type deterministic model,and used data from the Centers for Disease Control and Prevention(CDC),for the Ebola outbreak in Liberia and Sierra Leone.Two different data sets are available:one with raw reported data and one with corrected data(as the CDC suspects under-reporting).Results:Using a deterministic ordinary differential equation transmission model for Ebola epidemic,the basic reproductive ratio R0 for Liberia resulted to be 1.757 and 1.9 for corrected and uncorrected case data,respectively.For Sierra Leone,R0 resulted to be 1.492 and 1.362 for corrected and uncorrected case data,respectively.In each of the two cases we considered,the estimate for the basic reproductive ratio was initially greater than unity leading to an epidemic outbreak.Conclusion:We obtained robust estimates for the value of R0 associated with the 2014 Ebola outbreak,and showed that there is close agreement between our estimates of R0.Analysis of our model also showed that effective isolation is required,with the contact rate in isolation less than one quarter of that for the infected non-isolated population,and that the fraction of high-risk individuals must be brought to less than 10%of the overall susceptible population,in order to bring the value of R0 to less than 1,and hence control the outbreak. | Adnan Khan Mahim Naveed Muhammad Dur-e-Ahmad Mudassar Imran | 2015 | Infectious Diseases of Poverty2015，4，1： | 5 |
| 2 | Synthesis of plat- inum y-junction nanostructures using hierarchically designed alu- mina tern plates and their enhanced electrocatalytic activity for fuel-cell applications 显示文摘 | MAHIM S KANNAN R KOMATH I | 2008 | Chemistry of Materials2008，20，3： | 1 |
| 3 | Economies of scale in large containerships:optimal size and geographical implications显示文摘 | Kevin Cullinane Mahim Khanna | 2000 | Department of Shipping and Transport Logistics2000，，8： | 1 |
| 4 | Anewmethodbasedonparticleswarm optimization,antcolonyoptimizationand3-optalgorithmsfortravelingsalesmanproblem显示文摘 | MAHIM BAYKANOK KODAZH | 2015 | AppliedSoftComputing2015，30，： | 1 |
| 5 | Screening and identification of bioactive compounds from citrus against non-structural protein 3 protease of hepatitis C virus genotype 3a by fluorescence resonance energy transfer assay and mass spectrometry显示文摘BACKGROUND Hepatitis C virus genotype 3a(HCV G3a)is highly prevalent in Pakistan.Due to the elevated cost of available Food and Drug Administration-approved drugs against HCV,medicinal natural products of potent antiviral activity should be screened for the cost-effective treatment of the disease.Furthermore,from natural products,active compounds against vital HCV proteins like non-structural protein 3(NS3)protease could be identified to prevent viral proliferation in the host.AIM To develop cost-effective HCV genotype 3a NS3 protease inhibitors from citrus fruit extracts.METHODS Full-length NS3 without co-factor non-structural protein 4A(NS4A)and codon optimized NS3 protease in fusion with NS4A were expressed in Escherichia coli.The expressed protein was purified by metal ion affinity chromatography and gel filtration.Citrus fruit extracts were screened using fluorescence resonance energy transfer(FRET)assay against the protease and polyphenols were identified as potential inhibitors using electrospray ionization-mass spectrometry(MS)/MS technique.Among different polyphenols,highly potent compounds were screened using molecular modeling approaches and consequently the most active compound was further evaluated against HCV NS4A-NS3 protease domain using FRET assay.RESULTS NS4A fused with NS3 protease domain gene was overexpressed and the purified protein yield was high in comparison to the lower yield of the full-length NS3 protein.Furthermore,in enzyme kinetic studies,NS4A fused with NS3 protease proved to be functionally active compared to full-length NS3.So it was concluded that co-factor NS4A fusion is essential for the purification of functionally active protease.FRET assay was developed and validated by the half maximal inhibitory concentration(IC50)values of commercially available inhibitors.Screening of citrus fruit extracts against the native purified fused NS4A-NS3 protease domain showed that the grapefruit mesocarp extract exhibits the highest percentage inhibition 91%of protease activity.Among the compounds identified by LCMS analysis,hesperidin showed strong binding affinity with the protease catalytic triad having S-score value of-10.98.CONCLUSION Fused NS4A-NS3 protease is functionally more active,which is effectively inhibited by hesperidin from the grapefruit mesocarp extract with an IC50 value of 23.32μmol/L. | Mahim Khan Waqar Rauf Fazal-e-Habib Moazur Rahman Mazhar Iqbal | 2020 | World Journal of Hepatology2020，12，11： | 0 |
| 6 | 信息技术助力后疫情时代行业复苏显示文摘毋庸置疑,当前的全球卫生危机带给全球大多数行业难以置信的、似乎无法克服的挑战。值得庆幸的是,我们现在生活在一个技术如此先进的世界里,大多数领域都可以转向数字领域,并可转向各种创新中去继续运营。让我们来看看新科技将如何帮助我们从疫情中恢复! | Mahim Gupta 吉元(翻译) 郑艺龙(整理) | 2021 | 中国教育网络2021，，1： | 0 |
| 7 | Retraction Note:Screening and identification of bioactive compounds from citrus against non-structural protein 3 protease of hepatitis C virus genotype 3a by fluorescence resonance energy transfer assay and mass spectrometry显示文摘Retraction note:Khan M,Rauf W,Habib F,Rahman M,Iqbal M.Screening and identification of bioactive compounds from citrus against non-structural protein 3 protease of hepatitis C virus genotype 3a by fluorescence resonance energy transfer assay and mass spectrometry.World J Hepatol 2020;12(11):976-992 PMID:33312423 DOI:10.4254/wjh.v12.i11.976.The online version of the original article can be found at http://gffzz01c13a3805cf4476skon06cfbnb5v6qco.ffgz.tsg.suse.edu.cn/1948-5182/full/v12/i11/976.htm. | Mahim Khan Waqar Rauf Fazal-E-Habib Moazur Rahman Mazhar Iqbal | 2022 | World Journal of Hepatology2022，14，7： | 0 |
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