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| 1 | MYB Transcription Factors as Regulators of Phenylpropanoid Metabolism in Plants显示文摘导出 Phenylpropanoid 的混合物代表从本氨基丙酸发源的第二等的代谢物的一个多样的家庭。这些混合物对关於生命、不能生活的应力在植物生长和开发,并且在防卫有角色。许多这些混合物对人的健康和福利也有益。V-myb myeloblastosis 病毒的 oncogene 相当或相同的事物(MYB ) 蛋白质属于抄写因素的一个大家庭并且是导出 phenylpropanoid 的混合物的合成的关键管理者。这评论在植物在 phenylpropanoid 新陈代谢的规定总结 MYB 蛋白质和他们的角色的当前的理解。 | Jingying Liu Anne Osbourm Pengda Ma | 2015 | Molecular Plant2015，8，5： | 79 |
| 2 | The SmMYB36-SmERF6/SmERF115 module regulates the biosynthesis of tanshinones and phenolic acids in salvia miltiorrhiza hairy roots显示文摘Tanshinone and phenolic acids are the most important active substances of Salvia miltiorrhiza,and the insight into their transcriptional regulatory mechanisms is an essential process to increase their content in vivo.SmMYB36 has been found to have important regulatory functions in the synthesis of tanshinone and phenolic acid;paradoxically,its mechanism of action in S.miltiorrhiza is not clear.Here,we demonstrated that SmMYB36 functions as a promoter of tanshinones accumulation and a suppressor of phenolic acids through the generation of SmMYB36 overexpressed and chimeric SmMYB36-SRDX(EAR repressive domain)repressor hairy roots in combination with transcriptomic-metabolomic analysis.SmMYB36 directly down-regulate the key enzyme gene of primary metabolism,SmGAPC,up-regulate the tanshinones biosynthesis branch genes SmDXS2,SmGGPPS1,SmCPS1 and down-regulate the phenolic acids biosynthesis branch enzyme gene,SmRAS.Meanwhile,SmERF6,a positive regulator of tanshinone synthesis activating SmCPS1,was up-regulated and SmERF115,a positive regulator of phenolic acid biosynthesis activating SmRAS,was down-regulated.Furthermore,the seven acidic amino acids at the C-terminus of SmMYB36 are required for both self-activating domain and activation of target gene expression.As a consequence,this study contributes to reveal the potential relevance of transcription factors synergistically regulating the biosynthesis of tanshinone and phenolic acid. | Qi Li Xin Fang Ying Zhao Ruizhi Cao Juane Dong Pengda Ma | 2023 | Horticulture Research2023，10，1： | 2 |
| 3 | 2,3-Dihydroxybiphenyl dioxygenase gene was first discovered in Arthrobacter sp. strain PJ3显示文摘Bacterium strain PJ3,isolated from wastewater and identified as Arthrobacter sp. bacterium based on its 16S rDNA gene,could use carbazole as the sole carbon,nitrogen and energy source. The genomic library of strain PJ3 was constructed and a positive clone JM109(pUCW402) was screened out for the expression of dioxygenase by the ability to form yellow ring-fission product. A 2,3-dihydroxybiphenyl dioxygenase(23DHBD) gene of 933 bp was found in the 3360 bp exogenous fragment of pUCW402 by GenSCAN software and BLAST analysis. The phylogenetic analysis showed that 23DHBD from strain PJ3 formed a deep branch separate from a cluster containing most known 23DHBD in GenBank. Southern hybridization confirmed for the first time that the 23DHBD gene was from the genomic DNA of Arthrobacter sp. PJ3. In order to test the gene function,recombinant bacterium BL21(pETW-8) was constructed to express 23DHBD. The expression level in BL21(pETW-8) was highest compared with the recombinant bacteria JM109(pUCW402) and strain PJ3. We observed that 23DHBD was not absolute specific. The enzyme activity was higher with 2,3-dihydroxybiphenyl as a substrate than with catechol. The substrate specificity assay suggested that 23DHBD was essential for cleavage of bi-cyclic aromatic compounds during the course of aromatic compound biodegradation in Arthrobacter sp. strain PJ3. | YANG MeiYing MA PengDa LI WenMing LIU JinYing LI Liang ZHU XiaoJuan WANG XingZhi | 2007 | Chinese Science Bulletin2007，52，9： | 1 |
| 4 | 4-methylumbelliferone (4-MU) enhances drought tolerance of apple by regulating rhizosphere microbial diversity and root architecture显示文摘The dwarfing rootstocks-mediated high-density apple orchard is becoming the main practice management.Currently,dwarfing rootstocks are widely used worldwide,but their shallow root system and drought sensitivity necessitate high irrigation requirements.Here,the root transcriptome and metabolome of dwarfing(M9-T337,a drought-sensitive rootstock)and vigorous rootstocks(Malus sieversii,a drought-tolerant species,is commonly used as a rootstock)showed that a coumarin derivative,4-Methylumbelliferon(4-MU),was found to accumulate significantly in the roots of vigorous rootstock under drought condition.When exogenous 4-MU was applied to the roots of dwarfing rootstock under drought treatment,the plants displayed increased root biomass,higher root-to-shoot ratio,greater photosynthesis,and elevated water use efficiency.In addition,diversity and structure analysis of the rhizosphere soil microbial community demonstrated that 4-MU treatment increased the relative abundance of putatively beneficial bacteria and fungi.Of these,Pseudomonas,Bacillus,Streptomyces,and Chryseolinea bacterial strains and Acremonium,Trichoderma,and Phoma fungal strains known for root growth,or systemic resistance against drought stress,were significantly accumulated in the roots of dwarfing rootstock after 4-MU treatment under drought stress condition.Taken together,we identified a promising compound—4-MU,as a useful tool,to strengthen the drought tolerance of apple dwarfing rootstock. | Dehui Zhang Jieqiang He Pengda Cheng Yutian Zhang Abid Khan Shicong Wang Zhongxing Li Shuang Zhao Xiangqiang Zhan Fengwang Ma Xuewei Li Qingmei Guan | 2023 | Horticulture Research2023，10，7： | 0 |
| 5 | The SmNPR4-SmTGA5 module regulates SA-mediated phenolic acid biosynthesis in Salvia miltiorrhiza hairy roots显示文摘Phenolic acids are the main bioactive compounds in Salvia miltiorrhiza,which can be increased by salicylic acid(SA)elicitation.However,the specific molecular mechanism remains unclear.The nonexpresser of PR genes 1(NPR1)and its family members are essential components of the SA signaling pathway.Here,we report an NPR protein,SmNPR4,that showed strong expression in hairy root after SA treatment,acting as a negative moderator of SA-induced phenolic acid biosynthesis in S.miltiorrhiza(S.miltiorrhiza).Moreover,a basic leucine zipper family transcription factor SmTGA5 was identified and was found to interact with SmNPR4.SmTGA5 activates the expression of phenolic acid biosynthesis gene SmTAT1 through binding to the as-1 element.Finally,a series of biochemical assays and dual gene overexpression analysis demonstrated that the SmNPR4 significantly inhibited the function of SmTGA5,and SA can alleviate the inhibitory effect of SmNPR4 on SmTGA5.Overall,our results reveal the molecular mechanism of salicylic acid regulating phenolic acid biosynthesis in S.miltiorrhiza and provide new insights for SA signaling to regulate secondary metabolic biosynthesis. | Meiling Ding Bin Zhang Shuo Zhang RongRong Hao Yu Xia Pengda Ma Juane Dong | 2023 | Horticulture Research2023，10，5： | 0 |
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