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| 1 | Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)显示文摘Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China. | WANG Yanfeng CHI Liang LIU Qinghua XIAO Yongshuang MA Daoyuan XIAO Zhizhong XU Shihong LI Jun | 2019 | Journal of Oceanology and Limnology2019，37，1： | 2 |
| 2 | Structural deformation, metallogenic epoch and genetic mechanism of the Woxi Au-Sb-W deposit, Western Hunan Province, South China显示文摘The remobilization,migration,precipitation,and enrichment of ore-forming elements are closely related to structures.Therefore,detailed regional and ore-field structural analyses are critical for determining the genesis of a mineral deposit.The Jiangnan Orogenic Belt(JOB)is an important gold polymetallic metallogenic belt in South China,which is characterized by multiple periods of gold mineralization in the Paleozoic and Mesozoic.However,the genesis of these gold polymetallic deposits is still not well understood due to a lack of systematic research on the regional geology,ore-controlling structures and metallogenic mechanism.In this study,a detailed structural survey at the surface and in the subsurface tunnels was conducted on the Woxi Au-Sb-W deposit,the genesis of which is relatively controversial among the gold polymetallic deposits in the JOB due to poor structural constraints.In addition,a wolframite U-Pb dating was carried out to further constrain the relationship between structures and mineralization.Based on the results of these studies,together with those from previous studies,it is proposed that the Woxi deposit and surrounding areas likely underwent six periods of regional deformation,which are constrained in time and geodynamic setting.Furthermore,we present a systematic discussion on the roles of ore-controlling structures in the transportation,distribution,and deposition of ore-forming elements and localization of orebodies.According to the wolframite dating results,structural analyses,and previous data,we propose that the Woxi Au-Sb-W deposit was formed in two stages during the Yanshanian:a W(wolframite)-Au mineralization stage at ca.140 Ma and an Au-Sb-W(scheelite)mineralization stage at<130 Ma.These mineralizing events are interpreted to have a tight relationship with tectonic reactivation,and the ore-forming fluids were derived from deep sources,including those of magmatic or metamorphic origins.The Woxi deposit can therefore be classified as an“intracontinental reactivation-type”,and the mineralization is related to lithospheric extension caused by plate retreat,retention,and delamination following the cessation of westward subduction of the Paleo-Pacific Plate beneath the East Asian continent. | Bin LI Deru XU Daoyuan BAI Guoxiang CHI Junfeng DAI Cheng GAO Teng DENG Shaohao ZOU Wen MA Guojian WANG Yuexin LING Guangqian ZENG Yinmin LI | 2022 | Science China Earth Sciences2022，65，12： | 2 |
| 3 | The viability, melanophore and embryo genesis of first- and second-generation hybrids between Paralichthys olivaceus and P. dentatus显示文摘 | Juan Sui Qinghua Liu Zhizhong Xiao Daoyuan Ma Shihong Xu Tao He Yifan Liu Yongshuang Xiao Jun Li | 2013 | Marine Biology Research2013，，2： | 1 |
| 4 | Single-nucleus profiling unveils a geroprotective role of the Foxo3 in primate skeletal muscle aging显示文摘Age-dependent loss of skeletal muscle mass and function is a feature of sarcopenia,and increases the risk of many aging-related metabolic diseases.Here,we report phenotypic and single-nucleus transcriptomic analyses of non-human primate skeletal muscle aging.A higher transcriptional fluctuation was observed in myonuclei relative to other interstitial cell types,indicating a higher susceptibility of skeletal muscle fiber to aging.We found a downregulation of Foxo3 in aged primate skeletal muscle,and identi-fied FOxo3 as a hub transcription factor maintaining skeletal muscle homeostasis.Through the establishment of a complementary experimental pipeline based on a human pluripotent stem cell-derived myotube model,we revealed that silence of Foxo3 accelerates human myotube senescence,whereas genetic activation of endogenous FOxO3 alleviates human myotube aging.Altogether,based on a combination of monkey skeletal muscle and human myotube aging research models,we unraveled the pivotal role of the FOxO3 in safeguarding primate skeletal muscle from aging,providing a comprehensive resource for the development of clinical diagnosis and targeted therapeutic interventions against human skeletal muscle aging and the onset of sarcopenia along with aging-relateddisorders. | Ying Jing Yuesheng Zuo Yang Yu Liang Sun Zhengrong Yu Shuai Ma Qian Zhao Guoqiang Sun Huifang Hu Jingyi Li Daoyuan Huang Lixiao Liu Jiaming Li Zijuan Xin Haoyan Huang Juan Carlos Izpisua Belmonte Weiqi Zhang Si Wang Jing Qu Guang-Hui Liu | 2023 | Protein & Cell2023，14，7： | 0 |
| 5 | CRL2^(APPBP2)-mediated TSPYL2 degradation counteracts human mesenchymal stem cell senescence显示文摘Cullin-RING E3 ubiquitin ligases(CRLs),the largest family of multi-subunit E3 ubiquitin ligases in eukaryotic cells,represent core cellular machinery for executing protein degradation and maintaining proteostasis.Here,we asked what roles Cullin proteins play in human mesenchymal stem cell(hMSC)homeostasis and senescence.To this end,we conducted a comparative aging phenotype analysis by individually knocking down Cullin members in three senescence models:replicative senescent hMSCs,Hutchinson-Gilford Progeria Syndrome hMSCs,and Werner syndrome hMSCs.Among all family members,we found that CUL2 deficiency rendered hMSCs the most susceptible to senescence.To investigate CUL2-specific underlying mechanisms,we then applied CRISPR/Cas9-mediated gene editing technology to generate CUL2-deficient human embryonic stem cells(hESCs).When we differentiated these into h MSCs,we found that CUL2 deletion markedly accelerates hMSC senescence.Importantly,we identified that CUL2 targets and promotes ubiquitin proteasome-mediated degradation of TSPYL2(a known negative regulator of proliferation)through the substrate receptor protein APPBP2,which in turn downregulates one of the canonical aging marker-P21^(waf1/cip1),and thereby delays senescence.Our work provides important insights into how CRL2^(APPBP2)-mediated TSPYL2 degradation counteracts hMSC senescence,providing a molecular basis for directing intervention strategies against aging and aging-related diseases. | Daoyuan Huang Qian Zhao Kuan Yang Jinghui Lei Ying Jing Hongyu Li Chen Zhang Shuai Ma Shuhui Sun Yusheng Cai Guibin Wang Jing Qu Weiqi Zhang Si Wang Guang-Hui Liu | 2024 | Science China(Life Sciences)2024，67，3： | 0 |
| 6 | The ontogeny of acidic digestive tract in larval turbot Scophthalmus maximus based on H+/K+-ATPase and pepsinogen显示文摘Acidic digestion is an important digestive process of marine fish.In fish stomach,two enzymes are involved in the secretion of hydrochloric acid(HCl)and proteomic digestion:H^(+)/K^(+)-ATPase and pepsinogen.However,the starting of digestive function in fish is still unclear.To reveal the details of acidic digestion of turbot Scophthalmus maximus in early development,a 40 day of turbot larvae culture was conducted.The H^(+)/K^(+)-ATPase gene from the turbot S.maximus(smH^(+)/K^(+)-ATPase)was identified and characterized.Based on our previous discription on pepsinogen of turbot S.maximus,we combined pepsinogen and H^(+)/K^(+)-ATPase and analyzed the mechanism of acidic digestion in turbot.Results show that the spatial and temporal expression profiles of H^(+)/K^(+)-ATPase agreed with pepsinogen A and C in turbot,indicating a synergetic action between H^(+)/K^(+)-ATPase and pepsinogen during the acidic digestion process.In addition,the turbot juveniles showed a faster growth after the expressions of H^(+)/K^(+)-ATPase gene and pepsinogen gene,demonstrating that pepsin had a higher digestive efficiency,for which a compound diet should be provided to the fish from Day 22 onward.This study provided a reference for biology research and aquaculture of turbot and other marine fishes. | Yanfeng WANG Shulan LIU Teng GUO Shihong XU Qinghua LIU Zhizhong XIAO Daoyuan MA Jun LI Liang CHI | 2023 | Journal of Oceanology and Limnology2023，41，3： | 0 |
| 7 | A single-nucleus transcriptomic atlas of primate testicular aging reveals exhaustion of the spermatogonial stem cell reservoir and loss of Sertoli cell homeostasis显示文摘The testis is pivotal for male reproduction,and its progressive functional decline in aging is associated with infertility.However,the regulatory mechanism underlying primate testicular aging remains largely elusive.Here,we resolve the aging-related cellular and molecular alterations of primate testicular aging by establishing a single-nucleus transcriptomic atlas.Gene-expression patterns along the spermatogenesis trajectory revealed molecular programs associated with attrition of spermatogonial stem cell reservoir,disturbed meiosis and impaired spermiogenesis along the sequential continuum.Remarkably,Sertoli cell was identified as the cell type most susceptible to aging,given its deeply perturbed age-associated transcriptional profiles.Concomitantly,downregulation of the transcription factor Wilms'Tumor 1(WTi),essential for Sertoli cell homeostasis,was associated with accelerated cellular senescence,disrupted tight junctions,and a compromised cell identity signature,which altogether may help create a hostile microenvironment for spermatogenesis.Collectively,our study depicts in-depth transcriptomic traits of non-human primate(NHP)testicular aging at single-cell resolution,providing potential diagnostic biomarkers and targets for therapeutic interventions against testicular aging and age-related male reproductive diseases. | Daoyuan Huang Yuesheng Zuo Chen Zhang Guoqiang Sun Ying Jing Jinghui Lei Shuai Ma Shuhui Sun Huifen Lu Yusheng Cai Weiqi Zhang Fei Gao Andy Peng Xiang Juan Carlos Izpisua Belmonte Guang-Hui Liu Jing Qu Si Wang | 2023 | Protein & Cell2023，14，12： | 0 |
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