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| 1 | 过瘤胃蛋氨酸、赖氨酸和组氨酸对泌乳奶牛生产性能的影响显示文摘试验旨在研究饲料中单独添加或综合添加过瘤胃(RP)蛋氨酸、赖氨酸和组氨酸及可代谢蛋白(MP)对泌乳后期奶牛生产性能的影响。试验选取72头荷斯坦奶牛,随机分为6组,试验期9周。奶牛随机分配到6个试验组中,2周作为1个变量周期:MPA组,根据美国国家科学研究委员会(2001)标准,在饲料中添加调节MP到243 g·d-1;MPD组,不添加MP,MP含量低于标准量54 g·d-1;MPDM组,在MPD组的基础上加入RP蛋氨酸;MPDL组,在MPD组的基础上加入RP赖氨酸;MPDH组,在MPD组的基础上加入组氨酸;MPDMLH组,在MPD组的基础上加入RP蛋氨酸、RP赖氨酸和RP组氨酸。试验结果表明,与MPA组对比,MPD组的干物质采食量(DMI)、产奶量、乳成分(乳脂肪、乳蛋白和乳糖)及能量校正的牛乳(ECM)、ECM饲料利用效率、牛奶和血浆中的N均降低。在MPD基础上补充RP赖氨酸可使乳蛋白率、血浆中葡萄糖浓度增加,且牛奶中N元素含量有增加的趋势;补充RP组氨酸可使DMI、牛奶浓缩蛋白增加,且乳脂肪、乳蛋白和ECM显著增加;补充3种RP氨基酸时,增加了乳蛋白率,使DMI和乳脂率有增加趋势,也提高了乳脂肪、乳蛋白含量和ECM及ECM饲料利用效率。与MPA相比,MPD组牛奶中N的含量有降低趋势,且血浆中必需氨基酸(除赖氨酸和蛋氨酸)浓度均降低。补充RP赖氨酸、RP蛋氨酸和RP组氨酸提高了血浆中蛋氨酸(除MPDM组)、赖氨酸和组氨酸浓度。饲喂MPD的奶牛血红蛋白浓度降低,血浆生长素含量高于MPA组。MPD组牛奶中总饱和脂肪酸浓度有高于MPA和MPDMLH组的趋势。MPD组的多元不饱和脂肪、牛奶中奇链与支链脂肪酸含量与MPA组相比,有降低趋势。试验结果表明,对于饲料中缺乏MP的奶牛,在饲料中添加组氨酸和添加3种RP氨基酸(赖氨酸、蛋氨酸和组氨酸)均有提高牛奶产量和牛奶组分含量的可能性。 | Giallongo F Harper M T Oh J Lopes J C Lapierre H Patton R A Parys C Shinzato I Hristov A N | 2016 | 饲料博览2016，0，7： | 4 |
| 2 | Proton nuclear magnetic resonance-based metabonomic models for non-invasive diagnosis of liver fibrosis in chronic hepatitis C:Optimizing the classification of intermediate fibrosis显示文摘AIM To develop metabonomic models(MMs), using 1 H nuclear magnetic resonance(NMR) spectra of serum, to predict significant liver fibrosis(SF: Metavir ≥ F2), advanced liver fibrosis(AF: METAVIR ≥ F3) and cirrhosis(C: METAVIR = F4 or clinical cirrhosis) in chronic hepatitis C(CHC) patients. Additionally, to compare the accuracy of the MMs with the aspartate aminotransferase to platelet ratio index(APRI) and fibrosis index based on four factors(FIB-4). METHODS Sixty-nine patients who had undergone biopsy in the previous 12 mo or had clinical cirrhosis were included. The presence of any other liver disease was a criterion for exclusion. The MMs, constructed using partial least squares discriminant analysis and linear discriminant analysis formalisms, were tested by cross-validation, considering SF, AF and C. RESULTS Results showed that forty-two patients(61%) presented SF, 28(40%) AF and 18(26%) C. The MMs showed sensitivity and specificity of 97.6% and 92.6% to predict SF; 96.4% and 95.1% to predict AF; and 100% and 98.0% to predict C. Besides that, the MMs correctly classified all 27(39.7%) and 25(38.8%) patients with intermediate values of APRI and FIB-4, respectively. CONCLUSION The metabonomic strategy performed excellently in predicting significant and advanced liver fibrosis in CHC patients, including those in the gray zone of APRI and FIB-4, which may contribute to reducing the need for these patients to undergo liver biopsy. | Andrea Dória Batista Carlos Jonnatan Pimentel Barros Tássia Brena Barroso Carneiro Costa Michele Maria Goncalves de Godoy Ronaldo Dionísio Silva Joelma Carvalho Santos Mariana Montenegro de Melo Lira Norma ThoméJucá Edmundo Pessoa de Almeida Lopes Ricardo Oliveira Silva | 2018 | World Journal of Hepatology2018，10，1： | 2 |
| 3 | Invasive f ungal rhinosinusitis in immuno compromised patients显示文摘 | Lopes r P Valeraf C | 2004 | Rhinology2004，42，： | 1 |
| 4 | Research on fatigue cracking growth parameters in asphaltic mixtures using computed tomography显示文摘 | D Braz R T Lopes L M G Motta | 2004 | Nclear Instruments and Methods in Physics Research B 2132004，，： | 1 |
| 5 | Effects of incorporating polycaprolactone and flax fiber into glycerol-plasticized pea starch 显示文摘 | OLAYIDE O F LOPE G T SATYANARAYAN P | 2011 | J Polym Environ2011，19，： | 1 |
| 6 | Adding protein context to the human protein -protein interaction network to reveal meaningful inter- actions显示文摘 | SCHAEFER M H LOPES T J S MAH N et at | 2013 | PLoS Comput Biol2013，9，10： | 1 |
| 7 | Ulcerative and nonulcerative forms of bladder pain syndrome/interstitial cystitis do not dif- fer in symptom intensity or response to onabotulinum toxin A 显示文摘 | Pinto R Lopes T Costa D | 2014 | Urology2014，83，5： | 1 |
| 8 | Olfactory neuroblastoma An immunohis-tochemical, ultrastructural,and flow cytometric study显示文摘 | Scheithauer BW Lopes MB | 1995 | Cancer1995，76，： | 1 |
| 9 | PI(4,5)P2 regulates the activation and desensitization of TRPM8 channels through the TRP domain显示文摘 | Rohacs T Lopes C M Michailidis I Logothetis D E | 2005 | Nat Neurosci2005，8，： | 1 |
| 10 | Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnides complex)to inorginanic pollu- tants exposure显示文摘 | Lopes P A Pinheiro T Santos M C | 2001 | Sci Total Environ2001，280，13： | 1 |
| 11 | Radiosurgery-induced microvascular alteratians precede necrosis of the brain neuropil 显示文摘 | Kamiryo T Lopes MB Kassell NF | 2001 | Neurosurgery2001，49，2： | 1 |
| 12 | Expression of survivin, an inhibitor of apoptosis protein, in tumors of the nervous system显示文摘 | Sasaki T Lopes MB Hankins GR | 2002 | Acta Neuropathol2002，104，1： | 1 |
| 13 | Leakage detection and location in gas pipelines through an LPV identification approach显示文摘 | Lopes S P Azevedo T P Ramos J A | 2011 | C in Nonlinear Science and Numerical Simulation2011，12，16： | 1 |
| 14 | Characterisation of ultrafiltration and nanofillxation membranes from rejections of neutral reference solutes using a model of asymmetric pores 显示文摘 | Morro A Pessoa De Amorim M T Lopes A | 2008 | Journal of Membrane Science2008，319，1： | 1 |
| 15 | Olfactory neuroblastoma animmunohistochemical,ultrastructural and flow cytometric study显示文摘 | Hirose T cheithauer BW Lopes MB | 1995 | Cancer1995，76，1： | 1 |
| 16 | The utility of MPEG-7 systems in audio-visual applications with multiple streams显示文摘 | Lopes R J Lindsay A T David H | 2003 | IEEE Transactions on Circuits and Systems for Video Technology2003，13，1： | 1 |
| 17 | Developments in the rat adjuvant arthritis model and its use in therapeutic evaluation of novelnoninvasive treatment by SOD in transfersomes 显示文摘 | Simoes S I Delgado T C Lopes R M | 2005 | J Controlled Release2005，103，2： | 1 |
| 18 | Endoscopic stenting versus surgical colostomy for themanagement of malignant colonic obstruction: comparison of hospital costs and clinical outcomes 显示文摘 | Varadarajulu S Roy A Lopes T | 2011 | Surg Endosc2011，5，1： | 1 |
| 19 | Determination ofammonium in marine waters using a gas diffusion multicommutedflow injection system with in-line prevention of metal hydroxidesprecipitation显示文摘 | Oliveira S M Lopes T Toth I V | 2009 | J Environ Monit2009，11，22： | 1 |
| 20 | Biological sulphate reduction and redox mediator effects on azo dye decolourisation in anaerobic-aerobic sequen- cing batch reactors显示文摘 | ALBUQUERQUE M G E LOPES A T SERRALHEIRO J M | 2005 | Enzyme and Microbial Technology2005，36，79： | 1 |
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