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| 1 | Dynamic Expression of mi RNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings显示文摘Grafting of cucumber is widely used to improve growth and tolerance to biotic and abiotic stresses. Micro RNAs(miRNAs) regulate plant growth and development, and respond to various stresses through negative, post-transcriptional regulation of the expression of their target genes. We grafted cucumber(Cucumis sativus) scions onto pumpkin(Cucurbita moschata) rootstocks to study the molecular mechanisms of miRNA-mediated graftinginduced responses to drought stress. The relative expressions of 17 selected miRNAs and their predicted target m RNAs were detected by quantitative real-time PCR(q RT-PCR) in leaves and roots of hetero-grafted cucumber seedlings(cucumber as scion and pumpkin as rootstock) and autografted cucumber seedlings(cucumber as scion and rootstock) after 24 hours of 15% PEG6000 treatment. Compared with the expression in leaves of auto-grafted cucumber seedlings, the expressions of most miRNAs in the leaves of hetero-grafted seedlings changed dynamically: induced under normal conditions, and reduced after 3 h of drought stress, and then induced after 8 h and 24 h of drought stress. Similarly, compared with the expression in roots of auto-grafted cucumber seedlings, the expressions of most miRNAs in the roots of hetero-grafted cucumber seedlings changed dynamically: reduced under normal condition, and still reduced after 1 h, but induced after 3 h and 8 h, then reduced significant y after24 h of drought stress. These results are useful for the functional analysis of miRNAs in the mediation of grafting-dependent drought tolerance. | LI Chaohan LI Yansu BAI Longqiang HE Chaoxing YU Xianchang | 2016 | Horticultural Plant Journal2016，2，1： | 5 |
| 2 | Interference of CsGPA1,theα-submit of G protein,reduces drought tolerance in cucumber seedlings显示文摘The G proteinα-subunit,GPA1,is an integral component of several signaling pathways in plants,including response to abiotic stress.However,the molecular mechanism behind these processes remains largely unknown in the cucumber plant(Cucumis sativus L.).In order to further understand the role of CsGPA1 in cucumber under drought stress,changes in plant growth,physiological parameters,and gene expression of CsAQPs were all measured under water stress induced by polyethylene glycol(PEG)using wild type(WT)and CsGPA1-interference(RNAi)cucumber seedlings.Our results demonstrated that the RNAi plants had lower drought tolerance,displaying seriously withered leaves,lower relative growth rate,lower root-shoot ratio,and lower root activity under drought stress compared to WT plants.Physiological studies indicated that the suppression of CsGPA1 weakened drought stress tolerance due to higherwater loss rate in the leaves,higher levels of hydrogen peroxide(H2O2),increased malondialdehyde(MDA)content,lower free proline content,lower soluble sugar content,lower soluble protein content,and decreased antioxidant enzyme activities.qRT-PCR analysis demonstrated that the interference of CsGPA1 up-regulated the expression of most AQP genes(except for CsPIP2;3 in leaves)and down-regulated the expression of CsPIP1;2,CsPIP1;4,CsPIP2;1,and CsPIP2;4 in roots under drought stress when compared to WT plants.Our results demonstrated that CsGPA1 could function as a positive regulator in drought stress response by decreasing the accumulation of reactive oxygen species(ROS),improving permeable potentials,and reducing water loss in cucumber plants. | Yumei Liu Qinghua Du Longqiang Bai Mintao Sun Yansu Li Chaoxing He Jun Wang Xianchang Yu Yan Yan | 2021 | Horticultural Plant Journal2021，7，3： | 3 |
| 3 | Sugars promote graft union development in the heterograft of cucumber onto pumpkin显示文摘The use of heterografts is widely applied for the production of several important commercial crops,but the molecular mechanism of graft union formation remains poorly understood.Here,cucumber grafted onto pumpkin was used to study graft union development,and genome-wide tempo-spatial gene expression at the graft interface was comprehensively investigated.Histological analysis suggested that resumption of the rootstock growth occurred after both phloem and xylem reconnection,and the scion showed evident callus production compared with the rootstock 3 days after grafting.Consistently,transcriptome data revealed specific responses between the scion and rootstock in the expression of genes related to cambium development,the cell cycle,and sugar metabolism during both vascular reconnection and healing,indicating distinct mechanisms.Additionally,lower levels of sugars and significantly changed sugar enzyme activities at the graft junction were observed during vascular reconnection.Next,we found that the healing process of grafted etiolated seedlings was significantly delayed,and graft success,xylem reconnection,and the growth of grafted plants were enhanced by exogenous glucose.This demonstrates that graft union formation requires the correct sugar content.Furthermore,we also found that graft union formation was delayed with a lower energy charge by the target of rapamycin(TOR)inhibitor AZD-8055,and xylem reconnection and the growth of grafted plants were enhanced under AZD-8055 with exogenous glucose treatment.Taken together,our results reveal that sugars play a positive role in graft union formation by promoting the growth of cucumber/pumpkin and provide useful information for understanding graft union healing and the application of heterografting in the future. | Li Miao Qing Li Tian-shu Sun Sen Chai Changlin Wang Longqiang Bai Mintao Sun Yansu Li Xing Qin Zhonghua Zhang Xianchang Yu | 2021 | Horticulture Research2021，8，1： | 3 |
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