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| 1 | DROUGHT-INDUCED UNKNOWN PROTEIN 1 positively modulates drought tolerance in cultivated alfalfa(Medicago sativa L.)显示文摘Alfalfa is the most widely cultivated perennial legume forage crop worldwide.Drought is one of the major environmental factors influencing alfalfa productivity.However,the molecular mechanisms underlying alfalfa responses to drought stress are still largely unknown.This study identified a drought-inducible gene of unknown function,designated as Medicago sativa DROUGHT-INDUCED UNKNOWN PROTEIN 1(MsDIUP1).MsDIUP1 was localized to the nucleus,chloroplast,and plasma membranes.Overexpression of MsDIUP1 in Arabidopsis resulted in increased tolerance to drought,with higher seed germination,root length,fresh weight,and survival rate than in wild-type(WT)plants.Consistently,analysis of MsDIUP1 over-expression(OE)alfalfa plants revealed that MsDIUP1 also increased tolerance to drought stress,accompanied by physiological changes including reduced malondialdehyde(MDA)content and increased osmoprotectants accumulation(free proline and soluble sugar),relative to the WT.In contrast,disruption of MsDIUP1 expression by RNA interference(RNAi)in alfalfa resulted in a droughthypersensitive phenotype,with a lower chlorophyll content,higher MDA content,and less osmoprotectants accumulation than that of the WT.Transcript profiling of alfalfa WT,OE,and RNAi plants during drought stress showed differential responses for genes involved in stress signaling,antioxidant defense,and osmotic adjustment.Taken together,these results reveal a positive role for MsDIUP1 in regulating drought tolerance. | Dong Luo Xi Zhang Jie Liu Yuguo Wu Qiang Zhou Longfa Fang Zhipeng Liu | 2023 | The Crop Journal2023，11，1： | 0 |
| 2 | Trophic Mode-Dependent Proteomic Analysis Reveals Functional Significance of Light- Independent Chlorophyll Synthesis in Synechocystis sp. PCC 6803显示文摘光合的模型有机体 Synechocystis sp。PCC 6803 能在不同营养的模式成长,取决于光和外长的器官的碳来源的可获得性。然而,便于房间差别的蛋白质侧面变化怎么在不同模式宣传,包括地没被调查。用等压的基于标签的量的 proteomics,我们同时识别了并且确定越过四个不同营养的模式的 45% Synechocystis proteome,即,自造营养物质,不是自养, photoheterotrophic,和 mixotrophic 模式。在是越过四个营养的模式表示的差别的 155 蛋白质之中,涉及氮吸收和光无关的叶绿素合成的蛋白质是戏剧性地在 mixotrophic 模式的 upregulated,有 P 察觉到的 II phosphorylation 碳和氮吸收地位。而且,用在光无关的叶绿素合成的一个变异的有缺陷者的功能的学习表明这条小径为在骑车的光 / 黑暗的照明政体下面的叶绿素累积是重要的,一条件 mimicking 天 / 夜里在某些自然产地骑车。一起,这些结果在 cyanobacterium 在营养的模式依赖者蛋白质表示上提供最全面的信息,并且在营养的生长揭示光无关的叶绿素合成的功能的意义。 | Longfa Fang Haitao Ge Xiahe Huana Ye Liu Min Lu Jinlong Wang Weiyang Chen Wu Xu Yingchun Wang | 2017 | Molecular Plant2017，10，1： | 0 |
| 3 | Comparative transcriptome analyses reveal that the MsNST1 gene affects lignin synthesis in alfalfa(Medicago sativa L.)显示文摘As the second most abundant natural polymer,accounting for approximately 30%of the organic carbon in the biosphere,lignin plays an essential role in plant development.However,a high lignin content affects the nutritional quality of alfalfa(Medicago sativa L.),the most widely cultivated perennial legume forage crop.Histological analysis indicated that G-lignin and S-lignin were present in the stem,leaf,and petiole of alfalfa,and the deposition of lignin increased gradually in descending internodes.Neutral detergent fiber(NDF),acid detergent fiber(ADF),and acid detergent lignin(ADL)contents continually increased from the top to the bottom of the stem,and ADL content showed a similar trend in leaves.Alfalfa leaves and stems from five different nodes(1,2,4,6,and 8)were used as materials to investigate molecular regulatory mechanisms in lignin synthesis by RNA sequencing.Respectively 8074 and 7752 differentially expressed genes(DEGs)were identified in leaves and stems,and 1694 DEGs were common to the two tissues.‘‘Phenylpropanoid biosynthesis”was the most enriched pathway in both leaves and stems,and 134 key regulatory genes in lignin synthesis were identified by a weighted gene co-expression network analysis.The NAC family transcription factor MsNST1 gene was highly expressed in old leaf and stem tissues.The deposition pattern of G-and S-lignin differed among M.truncatula wild-type,nst1 mutants,and overexpression lines,and the transcription levels of lignin synthesis genes such as HCT,F5H,and COMT in these three materials also differed.These results suggest that MsNST1 affects lignin synthesis in alfalfa.These findings provide a genetic basis and abundant gene resources for further study of the molecular mechanisms of lignin synthesis,laying a foundation for low-lignin alfalfa breeding research. | Qiang Zhou Pei Mao Dong Luo Xutian Chai Hao Deng Qiangen Fang Longfa Fang Zhibiao Nan Jiangqi Wen Zhipeng Liu | 2022 | The Crop Journal2022，10，4： | 0 |
| 4 | The quantitative proteome atlas of a model cyanobacterium显示文摘Cyanobacteria are a group of oxygenic photosynthetic bacteria with great potentials in biotechnological applications and advantages as models for photosynthesis research. The subcellular localizations of the majority of proteins in any cyanobacteria remain undetermined, representing a major challenge in using cyanobacteria for both basic and industrial researches. Here, using label-free quantitative proteomics, we map 2027 proteins of Synechocystis sp. PCC6803, a model cyanobacterium, to different subcellular compartments and generate a proteome atlas with such information. The atlas leads to numerous unexpected but important findings, including the predominant localization of the histidine kinases Hik33 and Hik27 on the thylakoid but not the plasma membrane. Such information completely changes the concept regarding how the two kinases are activated. Together, the atlas provides subcellular localization information for nearly 60% proteome of a model cyanobacterium, and will serve as an important resource for the cyanobacterial research community. | Jinlong Wang Xiahe Huang Haitao Ge Yan Wang Weiyang Chen Limin Zheng Chengcheng Huang Haomeng Yang Lingyu Li Na Sui Yu Wang Yuanya Zhang Dandan Lu Longfa Fang Wu Xu Yuqiang Jiang Fang Huang Yingchun Wang | 2022 | Journal of Genetics and Genomics2022，49，2： | 0 |
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