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| 1 | Multimodality treatment in hepatocellular carcinoma patients with tumor thrombi in portal vein显示文摘AIM To compare the therapeutic effect andsignificances of multimodality treatment forhepatocellular carcinoma (HCC) with tumorthrombi in portal vein (PVTT).METHODS HCC patients (n = 147) with tumortrombi in the main portal vein or the first branchof portal vein were divided into four groups bythe several therapeutic methods. There wereconservative treatment group in 18 out ofpatients (group A); and hepatic artery ligation(HAL) and/or hepatic artery infusion (HAl)group in 18 patients (group B), in whompostoberative chemoembolization was doneperiodically; group of removal of HCC with PVTTin 79 (group C) and group of transcatheterhepatic arterial chemoembolization (TACE) orHAl and/or portal vein infusion (PVI) afteroperation in 32 (group D).RESULTS The median survival period was 12months in our series and the 1-, 3-, and 5-yearsurvival rates were 44.3%, 24.5% and 15.2%,respectively. The median survival times were 2,5, 12 and 16 months in group A, B, C and D,respectively. The 1-, 3- and 5-year survival rateswere 5.6%, 0% and 0% in group A; 22.2%,5.6% and 0% in group B; 53.9%, 26.9% and16.6% in group C; 79.3%, 38.9% and 26.8% ingroup D, respectively. Significant differenceappeared in the survival rates among the groups(P<0.05).CONCLUSION Hepatic resection with removalof tumor thrombi and HCC should increase thecurative effects and be encouraged for theprolongation of life span and quality of life forHCC patients with PVTT, whereas the besttherapeutic method for HCC with PVTT is withregional hepatic chemotherapy orchemoemblization after hepatic resection withremoval of tumor thrombi. | Jia Fan Zhi Quan Wu Zhao You Tang Jian Zhou Shuang Jian Qiu Zeng Chen Ma Xin Da Zhou Sheng Long Ye Liver Cancer Institute, Zhongshan Hospital, Fudan University Medical Center (Former Shanghai University), 136 Yixueyuan Road, Shanghai 200032, China | 2001 | World Journal of Gastroenterology2001，7，1： | 80 |
| 2 | Detection of HBV DNA and its existence status in liver tissues and peripheral blood lymphocytes from chronic hepatitis B patients显示文摘INTRODUCTIONCumulativeclinicalandexperimentalevidenceindicatesthathostimmuneresponsestohepatitisBvirus(HBV)areresponsibleforl... | TANG Ren Xian, GAO Feng Guang, ZENG Ling Yu, WANG Ying Wei and WANG Yu Long | 1999 | World Journal of Gastroenterology1999，5，4： | 27 |
| 3 | Optimal allocation of multi-type FACTS devices in power systems based on power flow entropy显示文摘Flexible AC transmission systems(FACTS)devices can effectively optimize the distribution of power flow.Power flow entropy can be applied as a measure of load distribution.In this paper,a method is proposed to optimize the distribution of power flow with the coordination of multi-type FACTS devices and establishes the corresponding mathematical models.The modified group searcher optimization(GSO)algorithm is proposed,in which the angle search is combined with chaotic search model to avoid jumping into local optimization.Compared with the different optimal allocation of multi-FACTS devices,the optimal allocation of multi-FACTS devices is achieved under the economic constraints.The locations obtained by this method can achieve the purpose of balancing power flow and enhancing the system performances.The simulations are demonstrated in an IEEE 118-bus power system with two classical types of FACTS,namely static var compensator(SVC)and thyristor controlled series Compensator(TCSC).The simulation results show that the proposed method is feasible and effective. | Canbing LI Liwu XIAO Yijia CAO Qianlong ZHU Baling FANG Yi TAN Long ZENG | 2014 | Journal of Modern Power Systems and Clean Energy2014，2，2： | 23 |
| 4 | LncRNA SNHG12 ameliorates brain microvascular endothelial cell injury by targeting miR-199a显示文摘Long non-coding RNAs regulate brain microvascular endothelial cell death, the inflammatory response and angiogenesis during and after ischemia/reperfusion and oxygen-glucose deprivation/reoxygenation(OGD/R) insults. The long non-coding RNA, SNHG12, is upregulated after ischemia/reperfusion and OGD/R in microvascular endothelial cells of the mouse brain. However, its role in ischemic stroke has not been studied. We hypothesized that SNHG12 positively regulates ischemic stroke, and therefore we investigated its mechanism of action. We established an OGD/R mouse cell model to mimic ischemic stroke by exposing brain microvascular endothelial cells to OGD for 0, 2, 4, 8, 16 or 24 hours and reoxygenation for 4 hours. Quantitative real-time polymerase chain reaction showed that SNHG12 levels in brain microvascular endothelial cells increased with respect to OGD exposure time. Brain microvascular endothelial cells were transfected with pc DNA-control, pc DNA-SNHG12, si-control, or si-SNHG12. After exposure to OGD for 16 hours, these cells were then analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, trypan blue exclusion, western blot, and capillary-like tube formation assays. Overexpression of SNHG12 inhibited brain microvascular endothelial cell death and the inflammatory response but promoted angiogenesis after OGD/R, while SNHG12 knockdown had the opposite effects. miR-199a was identified as a target of SNHG12, and SNHG12 overexpression reversed the effect of miR-199a on brain microvascular endothelial cell death, the inflammatory response, and angiogenesis. These findings suggest that SNHG12 suppresses endothelial cell injury induced by OGD/R by targeting miR-199a. | Fa-Qing Long Qing-Jie Su Jing-Xia Zhou De-Sheng Wang Peng-Xiang Li Chao-Sheng Zeng, Yi Cai | 2018 | Neural Regeneration Research2018，13，11： | 21 |
| 5 | Efficacy of intramuscular matrine in the treatment of chronic hepatitis B显示文摘BACKGROUND: Hepatitis B virus (HBV) infection, a glo- bal public health problem, is the leading cause of cirrhosis and hepatocellular carcinoma (HCC) worldwide. There are more than 350 million HBV carriers in the world and up to one million die annually due to hepatitis B associated liv- er disease. So far no optimal treatment is available for pa- tients with chronic hepatitis B. In the paper we investigated the efficacy of intramuscular matrine in the treatment of chronic hepatitis B. METHODS: One hundred and twenty patients with chronic hepatitis B were randomly divided into matrine treatment group (n =60) and control group (n =60). The patients of the matrine group were given intramuscularly with matrine (an alkaloid extracted from a traditional Chinese herb Radix Sophorae Flavescentis by Guangzhou Ming Xing Pharmaceu cal Factory, Guangzhou, China) of 100 mg daily for 90 days in addition to conventional liver-protective drugs in- cluding glucurone, inosine, compound vitamin B and caryophyllin. The control group received conventional liv- er-protective drugs alone. Clinical manifestations and labo- ratory parameters including liver biochemistry and serum hepatitis B virus markers were monitored before and after treatment in the two groups. RESULTS: Significant differences were seen between the two groups in terms of improvement of clinical symptoms and signs, recovery of liver functions, and serum conver- sion from hepatitis Be antigen to HBe antibody and from positive to negative serum HBV DNA (P <0.05-0.01). The result of the matrine group was more marked than that of the control group. Serious side-effects were not observed except mild pain at the site of injection of matrine in a few patients. CONCLUSION: These results indicate that intramuscular matrine may be an economical, efficacious, safe drug for the treatment of chronic hepatitis B. | Yao Long, Xiao-Tian Lin, Kun-Lun Zeng and Lian Zhang Zhanjiang, China Department of Infectious Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China Haibin Centre Hospital, Zhanjiang 524005, China Centre of Hepatology of Nanfang Hospital, First Military Medical University, Guang- zhou 510515, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，1： | 21 |
| 6 | Clinical trial with traditional Chinese medicine intervention ''tonifying the kidney to promote liver regeneration and repair by affecting stem cells and their microenvironment'' for chronic hepatitis B-associated liver failure显示文摘AIM:To study the clinical efficacy of traditional Chinese medicine(TCM)intervention'tonifying the kidney to promote liver regeneration and repair by affecting stem cells and their microenvironment'('TTK')for treating liver failure due to chronic hepatitis B.METHODS:We designed the study as a randomized controlled clinical trial.Registration number of Chinese Clinical Trial Registry is Chi CTR-TRC-12002961.A total of 144 patients with liver failure due to infection with chronic hepatitis B virus were enrolled in this randomized controlled clinical study.Participants were randomly assigned to the following three groups:(1)a modern medicine control group(MMC group,36patients);(2)a'tonifying qi and detoxification'('TQD')group(72 patients);and(3)a'tonifying the kidney to promote liver regeneration and repair by affecting stem cells and their microenvironment'('TTK')group(36patients).Patients in the MMC group received general internal medicine treatment;patients in the'TQD'group were given a TCM formula'tonifying qi and detoxification'and general internal medicine treatment;patients in the'TTK'group were given a TCM formula of'TTK'and general internal medicine treatment.All participants were treated for 8 wk and then followed at 48 wk following their final treatment.The primaryefficacy end point was the patient fatality rate in each group.Measurements of various virological and biochemical indicators served as secondary endpoints.The one-way analysis of variance and the t-test were used to compare patient outcomes in the different treatment groups.RESULTS:At the 48-wk post-treatment time point,the patient fatality rates in the MMC,'TQD',and'TTK'groups were 51.61%,35.38%,and 16.67%,respectively,and the differences between groups were statistically significant(P<0.05).However,there were no significant differences in the levels of hepatitis B virus DNA or prothrombin activity among the three groups(P>0.05).Patients in the'TTK'group had significantly higher levels of serum total bilirubin compared to MMC subjects(339.40μmol/L±270.09μmol/L vs 176.13μmol/L±185.70μmol/L,P=0.014).Serum albumin levels were significantly increased in both the'TQD'group and'TTK'group as compared with the MMC group(31.30 g/L±4.77g/L,30.72 g/L±2.89 g/L vs 28.57 g/L±4.56 g/L,P<0.05).There were no significant differences in levels of alanine transaminase among the three groups(P>0.05).Safety data showed that there was one case of stomachache in the'TQD'group and one case of gastrointestinal side effect in the'TTK'group.CONCLUSION:Treatment with'TTK'improved the survival rates of patients with liver failure due to chronic hepatitis B.Additionally,liver tissue was regenerated and liver function was restored. | Han-Min Li Zhi-Hua Ye Jun Zhang Xiang Gao Yan-Ming Chen Xin Yao Jian-Xun Gu Lei Zhan Yang Ji Jian-Liang Xu Ying-He Zeng Fan Yang Lin Xiao Guo-Guang Sheng Wei Xin Qi Long Qing-Jing Zhu Zhao-Hong Shi Lian-Guo Ruan Jia-Yao Yang Chang-Chun Li Hong-Bin Wu Sheng-Duo Chen Xin-La Luo | 2014 | World Journal of Gastroenterology2014，20，48： | 20 |
| 7 | The M5.0 Suining-Tongnan (China) earthquake of 31 January 2010:A destructive earthquake occurring in sedimentary cover显示文摘Usually, only crystalline basement is strong enough to store the massive strain energy that can be released in a damaging earthquake. By contrast, sedimentary cover is weak, because of its relatively high porosity and fluids. Therefore, it generally cannot accumulate enough energy for strong earthquakes. On January 31, 2010, a M5.0 earthquake occurred near the border of Suining and Tongnan in China. It excited strong short-period Rayleigh waves Rg, indicative of its shallow focal depth. The focal depth is constrained to less than 4 km, most probably in the range of 1-3 km, by modeling amplitude dependence on the frequency and waveforms of teleseismic depth phases (pP, sP). Because the local Mesozoic sedimentary cover is about 6 km thick, this earthquake should have occurred in the sedimentary cover. Though some shallow earthquakes with magnitudes up to M4 occur in Paleozoic sediments, this earthquake is the first M5 event studied in Mesozoic sedimentary cover. This event provides a rare opportunity to study seismogenic processes of damaging earthquakes in sedimentary basins. | LUO Yan NI SiDao ZENG XiangFang XIE Jun CHEN Yong LONG Feng | 2011 | Chinese Science Bulletin2011，56，6： | 17 |
| 8 | Clinical value of preoperative methylated septin 9 in Chinese colorectal cancer patients显示文摘BACKGROUND The methylated septin 9(mSEPT9) assay was the first blood-based test approved by the United States Food and Drug Administration as a colorectal screening test.However, the diagnostic and prognostic role of preoperative mSEPT9 for colorectal cancer(CRC) in Chinese patients is still unknown.AIM To improve the understanding of diagnostic and prognostic factors, serum m SEPT9 was detected in Chinese CRC patients.METHODS A retrospective analysis of 354 cases, of which 300 had CRC and 54 were normal,was performed in China. Patients' characteristics, treatments, and laboratory data, including age, the date of surgery, Union for International Cancer Control(UICC) stages, distant metastasis(M), and so on, were collected. Methylation levels of SEPT9 were quantified by quantitative, methylation-specific polymerase chain reaction before surgery. In addition, the effects of mSEPT9 on the occurrence and prognosis of 330 CRC cases from The Cancer Genome Atlas(TCGA) database were evaluated using bioinformatics analyses. Potential prognostic factors for overall survival(OS) and progression-free survival(PFS)were evaluated by Kaplan-Meier univariate analysis.RESULTSIn Chinese CRC patients, positive mSEPT9 was strongly associated with advanced UICC stages, deeper invasion by the primary tumor, and more distant metastasis. Methylation levels of SEPT9 were stage-dependent and showed a stepwise increase in UICC stages(I–IV), primary tumor categories(T1–T4),regional node categories(N0–N2), and distant metastasis categories(M0–M1).The patients with positive mSEPT9 showed a tendency toward lower PFS. After analyzing TCGA clinical data, the high mSEPT9 group was found to be obviously correlated only with more distant metastasis. The patients with high mSEPT9 levels showed a tendency toward lower OS. Besides, nine meaningful mSEPT9 sites were found to provide guidance for the follow-up studies.CONCLUSION MSEPT9 analysis may add valuable information to current tumor staging. Serum m SEPT9 in Chinese CRC patients appears to offer promising novel prognostic markers and might be considered for monitoring CRC recurrence. | Xue Yang Zhi-Jie Xu Xi Chen Shuang-Shuang Zeng Long Qian Jie Wei Mei Peng Xiang Wang Wan-Li Liu Hong-Ying Ma Zhi-Cheng Gong Yuan-Liang Yan | 2019 | World Journal of Gastroenterology2019，25，17： | 16 |
| 9 | The cloning of Dmc1 cDNAs and a comparative study of its expression in different ploidy cyprinid fishes显示文摘Dmc1 (disrupted meiotic cDNA) is a functionally specific gene, which was firstly discovered in yeast and then found to encode a protein required for homologous chromosome synapsis during the process of meiosis. In this investigation, we cloned the partial cDNAs of Dmc1 of diploid red crucian carp, Japanese crucian carp, common carp, triploid crucian carp and allotetraploid hybrids by using a pair of degenerate primers based on the conservative sequence of amino acids of the DMC1 protein in yeast, mouse and human. The full length cDNAs were then obtained by rapid amplification of cDNA ends (RACE). Our data showed that the full length cDNAs of Dmc1 in the three diploid fishes are all 1375 bp long, while it is 1383 bp long in triploids and 1379 bp long in allotetraploids. And despite of the variation in length, all the cDNAs encode a protein of 342 amino acids. A high homology of 97.3% of the DMC1 protein can be drawn by comparing the amino acid sequences in the three diploids, which is also of 86%, 86% and 95% similarity to human, mouse and zebrafish, respectively. A comparative study of the expression pattern of Dmc1 was carried out by RT-PCR using specific primers against the same se-quences of coding regions in different ploidy cyprinid fishes, from which it was showed that Dmc1 was expressed only in gonads of these five kinds of fishes. The expression pattern of Dmc1 in both ovaries and testes from different ploidy fishes within breeding season was also studied by Real-time PCR, and the results showed that the expression of this gene was greatly different among the three different ploidy fishes, which was the highest of triploid and lowest of allotetraploids. The histological sections data showed matured gonads of both diploid red crucian carp and allotetraploids in breeding season, although the latter demonstrated a higher maturation, and no gonadal maturation could be observed in triploids. In conclusion, we suggest that Dmc1 is specifically expressed in the period of meiosis in all the ploidy cyprinid fishes and directly related with the development of gonad in a manner of ploidy-independent way. And further, the high expression of Dmc1 in female triploids might be associ-ated with abnormal meiosis and sterility. | TAO Min, LIU ShaoJun, LONG Yu, ZENG Chen, LIU JiFang, LIU LiangGuo, ZHANG Chun, DUAN Wei & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2008 | Science China(Life Sciences)2008，51，1： | 13 |
| 10 | Bedaquiline-containing regimens in patients with pulmonary multidrug-resistant tuberculosis in China:focus on the safety显示文摘Background:World Health Organization recommends countries introducing new drug and short treatment regimen for drug resistant tuberculosis(DR-TB)should develop and implement a system for active pharmacovigilance that allows for detection,reporting and management of adverse events.The aim of the study is to evaluate the frequency and severity of adverse events(AEs)of bedaquiline-containing regimen in a cohort of Chinese patients with multidrug-resistant(MDR)/extensively drug-resistant(XDR)-TB based on active drug safety monitoring(aDSM)system of New Drug Introduction and Protection Program(NDIP). | Jing-Tao Gao Juan Du Gui-Hui Wu Yi Pei Meng-Qiu Gao Leonardo Martinez Lin Fan Wei Chen Li Xie Yu Chen Hua Wang Long Jin Guo-Bao Li Pei-Lan Zong Yu Xiong Qian-Hong Wu Ming-Wu Li Xiao-Feng Yan Yan-Fang Miao Qing-Shan Cai Xin-Jie Li Da-Peng Bai Shu-Jun Geng Guo-Li Yang Pei-JunTang Yi Zeng Xiao-Hong Chen Tong-Xia Li Cui Cai Yun Zhou Ma Zhuo Jian-Yun Wang Wen-Long Guan Lin Xu Ji-Chan Shi Wei Shu Li-Li Cheng FeiTeng Yu-Jia Ning Shi-Heng Xie Yu-Xian Sun Li-Jie Zhang Yu-Hong Liu | 2021 | Infectious Diseases of Poverty2021，10，2： | 11 |
| 11 | INFLUENCE OF QUERCETIN AND X-RAY ON COLLAGEN SYNTHESIS OF CULTURED HUMAN KELOID-DERIVED FIBROBLASTS显示文摘Objective To investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism.Methods Collagen synthesis of cultured human keloid and normal fibroblasts were detected by hydroxyproline colorimetric analysis. Immunocytochemical staining was used to investigate collagen I and III expression. mRNA expression of collagen I and III, and transforming growth factor (TGF)-β1 were assayed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. Results Quercetin inhibited the collagen synthesis of both keloid and normal fibroblasts in a dose-dependent manner. Immunocytochemical staining indicated that collagen I and III were down-regulated by quercetin and X-ray (P<0.05), particularly collagen I (P<0.05). mRNA expression of both collagen I and III in quercetin groups significantly decreased compared with that in control group (P<0.05), especially in the group treated with both quercetin and X-ray (P<0.01). mRNA level of TGF-β1 gene was down-regulated by quercertin (P<0.05). Conclusions Quercetin will probably be one of the new medicines which could effectively treat keloid. Quercetin combined with X-ray could reduce the dose of radiation. | Xiao Long Xuan Zeng Fu-quan Zhang Xiao-jun Wang | 2006 | Chinese Medical Sciences Journal2006，21，3： | 11 |
| 12 | Physical modeling and spectrum spread analysis of surface clutter in forward scattering radar显示文摘In this paper, based on the processing results of measured clutter data in forward scattering radar, a special phenomenon that the clutter spectrum spread is insensitive to the wind speed and carrier frequency is observed. The pendulum model is first time introduced into the physical modeling and formation mechanism analysis of surface clutter, and the quantitative expression of clutter spectrum spread is analytically obtained. Under the assumption of pendulum model, the processing results of measured clutter data agree well with the results of theoretical analysis, which fully verifies the correctness of pendulum model and reveals the formation mechanism of surface clutter spectrum in forward scattering radar. Furthermore, according to the stability of clutter spectrum spread with respect to carrier frequencies and wind speed, the optimal cutofrequencies of clutter filter can be obtained, which establishes the basis for moving target detection and accurate parameters estimation. | LONG Teng, HU Cheng, ZENG Tao & LI XiaoLiang Radar Research Laboratory, Department of Electronic Engineering, Beijing Institute of Technology, Beijing 100081, China | 2010 | Science China(Information Sciences)2010，53，11： | 11 |
| 13 | Statistic characteristic analysis of forward scattering surface clutter in bistatic radar显示文摘In this paper,based on the processing results of measured forward scattering clutter data in bistatic radar,using the Gaussian distribution model,Exponential distribution model,Power-Law distribution model,high order Auto-Regressive model and so on,the probability density distribution and power spectrum mathematical model are obtained,and the impact of clutter statistic models on target detection performance is analytically derived from statistic theory.According to the estimated clutter probability distribution,power spectrum model and generalized matching filter theory,the forward scattering clutter in bistatic radar is effectively suppressed and the target motion parameters are accurately estimated from the measured clutter data. | HU Cheng ,LONG Teng & ZENG Tao Radar Research Lab,Department of Electronic Engineering,Beijing Institute of Technology,Beijing 100081,China | 2010 | Science China(Information Sciences)2010，53，12： | 11 |
| 14 | Interferon-c and interleukin-4 detected in serum and saliva from patients with oral lichen planus显示文摘Our previous salivary study had demonstrated an apparent T helper 2(Th2)-predominance in saliva of oral lichen planus(OLP) patients and suggested a potential of salivary interleukin-4(IL-4) as a biomarker for monitoring disease severity. To further determine the consistency of Th1/Th2 bias of OLP, this study investigated the expression profile of interferon-c(IFN-c) and IL-4 in serum and the relationship of the serum levels of these cytokines with their saliva partners. Sixty ethnic Chinese patients with OLP(40 of the erythematous/ulcerative form and 20 of the reticular form) were recruited for this study, with 40 age–sex-matched healthy volunteers as control group. IFN-c and IL-4 levels in serum and paired saliva samples were screened by enzyme-linked immunosorbent assay. OLP patient showed a low-level IFN-c but high-level IL-4 expression profile in both serum and saliva, with a lower IFN-c/IL-4 ratio. Serum IL-4 level in the erythematous/ulcerative group was significantly higher than that in the reticular group. Serum levels of IFN-c and IL-4 were significantly and positively correlated with their saliva partners. These results provided more evidence for Th2 cytokinepredominant immune imbalance in OLP, as well as the potential of IL-4 as the biomarker for monitoring severity of OLP. | Wen-Zhao Liu Ming-Jing He Long Long Dong-Liang Mu Ming-Shu Xu Xue Xing Xin Zeng Ga Liao Hong-Xia Dan Qian-Ming Chen | 2014 | International Journal of Oral Science2014，6，1： | 10 |
| 15 | Characteristics of secondary inorganic aerosol and sulfate species in size-fractionated aerosol particles in Shanghai显示文摘Sulfate, nitrate and ammonium(SNA) are the dominant species in secondary inorganic aerosol, and are considered an important factor in regional haze formation. Size-fractionated aerosol particles for a whole year were collected to study the size distribution of SNA as well as their chemical species in Shanghai. SNA mainly accumulated in fine particles and the highest average ratio of SNA to particulate matter(PM) was observed to be 47% in the fine size fraction(0.49–0.95 μm). Higher sulfur oxidation ratio and nitrogen oxidation ratio values were observed in PM of fine size less than 0.95 μm. Ion balance calculations indicated that more secondary sulfate and nitrate would be generated in PM of fine size(0.49–0.95 μm). Sulfur K-edge X-ray absorption near-edge structure(XANES) spectra of typical samples were analyzed. Results revealed that sulfur mainly existed as sulfate with a proportion(atomic basis) more than 73% in all size of PM and even higher at 90% in fine particles. Sulfate mainly existed as(NH4)2SO4 and gypsum in PM of Shanghai. Compared to non-haze days, a dramatic increase of(NH4)2SO4 content was found in fine particles on haze days only, which suggested the promoting impact of(NH4)2SO4 on haze formation. According to the result of air mass backward trajectory analysis, more(NH4)2SO4 would be generated during the periods of air mass stagnation. Based on XANES, analysis of sulfate species in size-fractionated aerosol particles can be an effective way to evaluate the impact of sulfate aerosols on regional haze formation. | Shilei Long Jianrong Zeng Yan Li Liangman Bao Lingling Cao Ke Liu Liang Xu Jun Lin Wei Liu Guanghua Wang Jian Yao Chenyan Ma Yidong Zhao | 2014 | Journal of Environmental Sciences2014，26，5： | 10 |
| 16 | Induction of gynogenesis in red crucian carp using spermatozoa of blunt snout bream显示文摘Gynogenetic 双在红鲫鱼鲤鱼(RCC )(Carassius auratus 红变化) 被导致从钝猪嘴鲷(B)(Megalabrama amblycephala ) 或从镜子鲤鱼(C) 的鸡蛋使用紫外照耀精子(Cyprinus carpio。L) 。精子被适当紫外剂量遗传上使失去活性,然后母亲的 DNA 在 0-4 ° C 与冷吃惊被复制。当使用 B 的精子时,起初喂的授精率,孵化的率和幸存是 52.6 ± 3.0 %, 23.6 ± 4.1 %和 15.7 ± 3.4 %分别地,并且起初喂的幸存比那显著地高( 11.3 ± 2.2%)当使用 C 的精子时( Cypripnus carpio 。L) 。根据词法特征,染色体数字和 gonadal 开发的度, gynogenetic RCC 能与 RCC ♀
的控制混血儿被区分开来B♂ 。有红身体颜色, 100 个染色体和正常 gonadal 开发的个人是 syccessful gyogenetic RCC,当有 124 或 148 个染色体和推迟的 gonadal 开发的个人是混血儿时(RCC B ) 。三倍性的混血儿(RCC B )(2 岁) 是无菌的,但是 tetraploid 混血儿(RCC B ) 是二的性成熟的年龄。在现在的学习,与 C 的精子相比, B 的 spermatoxoa 的优点作为激活来源是那能增加起初 gynogentic 个人和 simplfy 喂 gynogenetic 的证实的幸存地位,它建议 B 的精子是为在鲫鱼鲤鱼导致 gynogenesis 的有效激活来源。 | SUN Yuandong TAO Min LIU Shaojun ZHANG Chun DUAN Wei SHEN Jiamin WANG Jing ZENG Chen LONG Yu LIU Yun | 2007 | Progress in Natural Science:Materials International2007，17，2： | 10 |
| 17 | Genome of Plant Maca (Lepidium meyenil) Illuminates Genomic Basis for High-Altitude Adaptation in the Central Andes显示文摘Maca (Lepidium meyenii Walp, 2n = 8x = 64 ) ,属于 Brassicaceae 家庭,经济植物在秘鲁(4000-4500 m ) 在中央安第斯山脉岭被栽培。认为快速中央安第斯山脉高举发生 5-10 百万年以前(妈) ,一个进化问题关于象 maca 那样的植物怎么在一个短地质的时期以内获得高高度的改编产生。这里,我们报导 maca 的高质量的染色体集会,在里面它二仔细 spaced maca 特定的整个染色体的复制(WGD;6.7 妈) 被识别。在 maca 和密切相关的 Brassicaceae 种类之间的比较 genomic 分析揭示了经由 WGD 涉及不能生活的压力反应,荷尔蒙发信号小径,和第二等的代谢物生合成的 maca 基因和基因家庭的扩大。保留和许多复制基因的随后的功能的分叉可以说明词法、生理的变化(即,小叶形状和自我富饶) 在在高高度的环境的 maca。另外,一些复制 maca 基因在词法改编与功能被识别(即,叶卷应答) 并且不能生活的压力反应(即, GLYCINE 富有的 RNA 有约束力的蛋白质和 DNA-DAMAGE-REPAIR/TOLERATION 2 ) 在积极选择下面。一起, maca 染色体提供有用信息在安第斯山脉在植物的高高度的改编理解 WGD 的重要角色。 | Jing Zhang Yang Tian Liang Yan GuanghuiZhang Xiao Wang Yan Zeng Jiajin Zhang Xiao Ma Yuntao Tan Ni Long Yangzi Wang Yujin Ma Yuqi He Yu Xue Shumei Hao Shengchao Yang Wen Wang Liangsheng Zhang Yang Dong Wei Chen Jun Sheng | 2016 | Molecular Plant2016，9，7： | 9 |
| 18 | Xa7, a new executor R gene that confers durable and broad-spectrum resistance to bacterial blight disease in rice显示文摘Bacterial blight(BB)is a globally devastating rice disease caused by Xanthomonas oryzae pv.oryzae(Xoo).The use of disease resistance(R)genes in rice breeding is an effective and economical strategy for the control of this disease.Nevertheless,a majority of R genes lack durable resistance for long-term use under global warming conditions.Here,we report the isolation of a novel executor R gene,Xa7,that confers extremely durable,broad-spectrum,and heat-tolerant resistance to Xoo.The expression of Xa7 was induced by incompatible Xoo strains that secreted the transcription activator-like effector(TALE)AvrXa7 or PthXo3,which recognized effector binding elements(EBEs)in the Xa7 promoter.Furthermore,Xa7 induction was faster and stronger under high temperatures.Overexpression of Xa7 or co-transformation of Xa7 with avrXa7 triggered a hypersensitive response in plants.Constitutive expression of Xa7 activated a defense response in the absence of Xoo but inhibited the growth of transgenic rice plants.In addition,analysis of over 3000 rice varieties showed that the Xa7 locuswas found primarily in the indica and aus subgroups.A variation consisting of an 11-bp insertion and a base substitution(G to T)was found in EBEAvrXa7 in the tested varieties,resulting in a loss of Xa7 BB resistance.Through a decade of effort,we have identified an important BB resistance gene and characterized its distinctive interaction with Xoo strains;these findings will greatly facilitate research on the molecular mechanism of Xa7-mediated resistance and promote the use of this valuable gene in breeding. | Xifeng Chen Pengcheng Liu Le Mei Xiaoling He Long Chen Hui Liu Shurong Shen Zhandong Ji Xixi Zheng Yuchen Zhang Zhenyu Gao Dali Zeng Qian Qian Bojun Ma | 2021 | Plant Communications2021，2，3： | 9 |
| 19 | Sulfur speciation and bioaccumulation in camphor tree leaves as atmospheric sulfur indicator analyzed by synchrotron radiation XRF and XANES显示文摘Analyzing and understanding the effects of ambient pollution on plants is getting more and more attention as a topic of environmental biology.A method based on synchrotron radiation X-ray fluorescence and X-ray absorption near edge structure spectroscopy was established to analyze the sulfur concentration and speciation in mature camphor tree leaves (CTLs),which were sampled from 5 local fields in Shanghai,China.Annual SO2 concentration,SO42-concentration in atmospheric particulate,SO42-and sulfur concentration in soil were also analyzed to explore the relationship between ambient sulfur sources and the sulfur nutrient cycling in CTLs.Total sulfur concentration in mature camphor tree leaves was 766-1704 mg/kg.The mainly detected sulfur states and their corresponding compounds were +6 (sulfate,include inorganic sulfate and organic sulfate),+5.2 (sulfonate),+2.2 (suloxides),+0.6 (thiols and thiothers),+0.2 (organic sulfides).Total sulfur concentration was strongly correlated with sulfate proportion with a linear correlation coefficient up to 0.977,which suggested that sulfur accumulated in CTLs as sulfate form.Reduced sulfur compounds (organic sulfides,thiols,thioethers,sulfoxide and sulfonate) assimilation was sufficed to meet the nutrient requirement for growth at a balanced level around 526 mg/kg.The sulfate accumulation mainly caused by atmospheric sulfur pollution such as SO2 and airborne sulfate particulate instead of soil contamination.From urban to suburb place,sulfate in mature CTLs decreased as the atmospheric sulfur pollution reduced,but a dramatic increase presented near the seashore,where the marine sulfate emission and maritime activity pollution were significant.The sulfur concentration and speciation in mature CTLs effectively represented the long-term biological accumulation of atmospheric sulfur pollution in local environment. | Jianrong Zeng Guilin Zhang Liangman Bao Shilei Long Mingguang Tan Yan Li Chenyan Ma Yidong Zhao | 2013 | Journal of Environmental Sciences2013，25，3： | 9 |
| 20 | Lentivirus vectors construction of SiRNA targeting interference GPC3 gene and its biological effects on liver cancer cell lines Huh-7显示文摘Objective:To build GPC3 gene short hairpin interference RNA(shRNA)slow virus veclor.observe expression of Huh-7 GPC3 gene in human liver cell line proliferation apoptosis and the effect of GPC3 gene influencing on liver cancer cell growth,and provide theoretical basis for genc therapy of liver cancer.Methods:Hepatocellular carcinoma cell line Huh-7 wsa transfected by a RNA interference technique.GPC3 gene expression in a variety of liver cancer cell lines was detected by fluorescence quantitative PCR.Targeted GPC3 gene seqnences of small interfering RNA(siRNA)PGC-shRNA-GPC3 were restructured.Stable expression cell linse of siRNA were screened and established with the heplp of liposomes(lipofectamine^(TM2000))as carrier transfcetion of human liver cell lines.In order to validate siRNA interference efficiency.GPC3 siRNA mRNA expression was detected after transfection by using RT-PCR and Western blot.The absorbance value of the cells of blank group,untransfection group and transfection group,the cell cycle and cell apoptosis were calculated,and effects of GPC3 gene nn Huh-7 cell proliferation and apoptosis were observed.Results:In the liver cancer cell lines Huh-7 GPC3 gene showed high expression.PGC-shRNA-GPC3 recombinant plasmid was constructde successfully via sequencing validation.Stable recombinant plasmid transfected into liver cancer cell linse Huh-7can obviously inhibit GPC3 mRNA expression level.Conclusions:The targeted GPC3 siRNA can effectively inhibit the expression of GPC3. | Chang-Jiang Lei Chun Yao Qing-Yun Pan Hao-Cheng Long Lei Li Shu-Ping Zheng Cheng Zeng Jian-Bin Huang | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，10： | 8 |
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