| 1 | Analysis of Genetic Diversity of Vitis amurensis Rupr.(Vitaceae) Germplasm Resources显示文摘[Objective] This study aimed to explore the genetic diversity of Vitis amurensis Rupr. (Vitaceae) germplasm resources.[Method] Out of total 245 pairs of primers, 18 were selected for SSR amplification of 360V. amurensis experimental materials.[Result] The number of bands amplified by each primer ranged from 4 to 13 with a mean of 9.44. The length of bands ranged from 150 to 1000 bp, concentrated at 200-750bp. The 18 pairs of primers amplified 170 bands totally, of which 167 bands were polymorphic with a polymorphism ratio of 98.2%. The Shannon's diversity index (I) is 1.778 051. With the SSR-PCR amplification of 360V. amurensis varieties (strains), 5 specific bands were amplified by certain primers in several varieties(strains) accounting for 2.95% of the total bands.[Conclusion] SSR molecular marker technique was an efficient method to detect the genetic diversity of V. amurensis and thereby is an effective tool for pedigree analysis and variety identification of A. amurensis varieties(strains). | Jinghui WEN Hailin SHEN Liren ZOU Lei CHEN Hongzhang LIU | 2012 | Agricultural Biotechnology2012，1，5： | 0 |
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