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| 1 | Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice显示文摘Jasmonic acid(JA) and related metabolites play a key role in plant defense and growth. JA carboxyl methyltransferase(JMT) may be involved in plant defense and development by methylating JA to methyl jasmonate(Me JA) and thus influencing the concentrations of JA and related metabolites. However, no JMT gene has been well characterized in monocotyledon defense and development at the molecular level. After we cloned a rice JMT gene,Os JMT1, whose encoding protein was localized in the cytosol, we found that the recombinant Os JMT1 protein catalyzed JA to Me JA. Os JMT1 is up-regulated in response to infestation with the brown planthopper(BPH; Nilaparvata lugens). Plants in which Os JMT1 had been overexpressed(oeJMT plants) showed reduced height and yield. These oe-JMT plants also exhibited increased Me JA levels but reduced levels of herbivore-induced JA and jasmonoyl-isoleucine(JAIle). The oe-JMT plants were more attractive to BPH female adults but showed increased resistance to BPH nymphs,probably owing to the different responses of BPH female adults and nymphs to the changes in levels of H_2O_2 and Me JA in oe-JMT plants. These results indicate that Os JMT1,by altering levels of JA and related metabolites, plays a role in regulating plant development and herbivore-induced defense responses in rice. | Jinfeng Qi Jiancai Li Xiu Han Ran Li Jianqiang Wu Haixin Yu Lingfei Hu Yutao Xiao Jing Lu Yonggen Lou | 2016 | Journal of Integrative Plant Biology2016，58，6： | 10 |
| 2 | Progress in flexible organic thin-film transistors and integrated circuits显示文摘Organic thin-film transistor constructs the headstone of flexible electronic world such as conformable sensor arrays and flexible active-matrix displays. With solutionprocessed methods, it forges ahead toward large-area, lowcost manufacturing goals. As an indispensable complement to traditional silicon-based transistors, organic thin-film field-effect transistors have made great progress in materials,performance, bending capacity, and integrated circuits in recent few years. Flexible transistors and circuitry have extremely promising application prospects and possess irreplaceable status in foldable displays, artificial skins and bendable smart cards. In this review, we will discuss the evolution of flexible organic transistors and integrated circuits in terms of material, fabrication as well as application. | Congyan Lu Zhuoyu Ji Guangwei Xu Wei Wang Lingfei Wang Zhiheng Han Ling Li Ming Liu | 2016 | Science Bulletin2016，61，14： | 5 |
| 3 | Redox-sensitive micelles for targeted intracellular delivery and combination chemotherapy of paclitaxel and all-trans-retinoid acid显示文摘The application of paclitaxel(PTX) in clinic has been restricted due to its poor solubility.Several traditional nano-medicines have been developed to improve this defect,while they are still lack of tumor targeting ability and rapid drug release. In this work,an amphiphilic polymeric micelle of hyaluronic acid(HA) – all-trans-retinoid acid(ATRA) with a disulfide bond,was developed successfully for the co-delivery of PTX and ATRA. The combination chemotherapy of PTX and ATRA can strengthen the anti-tumor activity. Along with selfassembling to micelles in water,the delivery system displayed satisfying drug loading capacities for both PTX(32.62% ± 1.39%) and ATRA,due to directly using ATRA as the hydrophobic group. Rapid drug release properties of the PTX-loaded redox-sensitive micelles(HA-SS-ATRA) in vitro were confirmed under reducing condition containing GSH. Besides,HA-CD44 mediated endocytosis promoted the uptake of HA-SS-ATRA micelles by B16 F10 cells. Due to these properties,cytotoxicity assay verified that PTX-loaded HA-SS-ATRA micelles showed concentration-dependent cytotoxicity and displayed obvious combination therapy of PTX and ATRA. Importantly,HA-SS-ATRA micelles could remarkably prolong plasma circulation time after intravenously administration. Therefore,redox-sensitive HASS-ATRA micelles could be utilized and explored as a promising drug delivery system for cancer combination chemotherapy. | Lingfei Han Lejian Hu Fulei Liu XinWang Xiaoxian Huang Bowen Liu Feng Feng Wenyuan Liu Wei Qu | 2019 | Asian Journal of Pharmaceutical Sciences2019，14，5： | 2 |
| 4 | Melatonin shapes bacterial clearance function of porcine macrophages during enterotoxigenic Escherichia coli infection显示文摘Due to the immature gastrointestinal immune system,weaning piglets are highly susceptible to pathogens,e.g.,enterotoxigenic Escherichia coli(ETEC).Generally,pathogens activate the immune cells(e.g.,macrophages)and shape intracellular metabolism(including amino acid metabolism);nevertheless,the metabolic cues of tryptophan(especially melatonin pathway)in directing porcine macrophage function during ETEC infection remain unclear.Therefore,this study aimed to investigate the changes in the serotonin pathway of porcine macrophages during ETEC infection and the effect of melatonin on porcine macrophage functions.Porcine macrophages(3D4/21 cells)were infected with ETEC,and the change of serotonin pathway was analysed by reverse transcription PCR and metabolomic analysis.The effect of melatonin on porcine macrophage function was also studied with proteomic analysis.In order to investigate the effect of melatonin on bacterial clearance function of porcine macrophages during ETEC infection,methods such as bacterial counting,reverse transcription PCR and western blotting were used to detect the corresponding indicators.The results showed that ETEC infection blocked melatonin production in porcine macrophages(P<0.05)which is largely associated with the heat-stable enterotoxin b(STb)of ETEC(P<0.05).Interestingly,melatonin altered porcine macrophage functions,including bacteriostatic and bactericidal activities based on proteomic analysis.In addition,melatonin pretreatment significantly reduced extracellular lactate dehydrogenase(LDH)activity(P<0.05),indicating that melatonin also attenuated ETEC-triggered macrophage death.Moreover,melatonin pretreatment resulted in the decrease of viable ETEC in 3D4/21 cells(P<0.05),suggesting that melatonin enhances bacterial clearance of porcine macrophages.These results suggest that melatonin is particularly important in shaping porcine macrophage function during ETEC infection. | Lingfei Du Bingnan Liu Ziyi Han Yaoyao Xia Miaomiao Wu Shaojuan Liu | 2022 | Animal Nutrition2022，，4： | 2 |
| 5 | Plant variety and cultivar identification: advances and prospects显示文摘 | Korir Kibet Nicholas Han Jian Shangguan Lingfei | 2013 | Critical reviews in biotechnology2013，33，2： | 1 |
| 6 | Plant property regulates soil bacterial community structure under altered precipitation regimes in a semi-arid desert grassland, China显示文摘Variations of precipitation have great impacts on soil carbon cycle and decomposition of soil organic matter.Soil bacteria are crucial participants in regulating these ecological processes and vulnerable to altered precipitation.Studying the impacts of altered precipitation on soil bacterial community structure can provide a novel insight into the potential impacts of altered precipitation on soil carbon cycle and carbon storage of grassland.Therefore,soil bacterial community structure under a precipitation manipulation experiment was researched in a semi-arid desert grassland in Chinese Loess Plateau.Five precipitation levels,i.e.,control,reduced and increased precipitation by 40%and 20%,respectively(referred here as CK,DP40,DP20,IP40,and IP20)were set.The results showed that soil bacterial alpha diversity and rare bacteria significantly changed with altered precipitation,but the dominant bacteria and soil bacterial beta diversity did not change,which may be ascribed to the ecological strategy of soil bacteria.The linear discriminate analysis(LDA)effect size(LEfSe)method found that major response patterns of soil bacteria to altered precipitation were resource-limited and drought-tolerant populations.In addition,increasing precipitation greatly promoted inter-species competition,while decreasing precipitation highly facilitated inter-species cooperation.These changes in species interaction can promote different distribution ratios of bacterial populations under different precipitation conditions.In structural equation model(SEM)analysis,with changes in precipitation,plant growth characteristics were found to be drivers of soil bacterial community composition,while soil properties were not.In conclusion,our results indicated that in desert grassland ecosystem,the sensitive of soil rare bacteria to altered precipitation was stronger than that of dominant taxa,which may be related to the ecological strategy of bacteria,species interaction,and precipitation-induced variations of plant growth characteristics. | ZHANG Lihua GAO Han WANG Junfeng ZHAO Ruifeng WANG Mengmeng HAO Lianyi GUO Yafei JIANG Xiaoyu ZHONG Lingfei | 2023 | Journal of Arid Land2023，15，5： | 0 |
| 7 | Inwardly rectifying potassium channel 5.1:Structure,function,and possible roles in diseases显示文摘Inwardly rectifying potassium(Kir)channels make it easier for K^(+)to enter into a cell and subsequently regulate cellular biological functions.Kir5.1(encoded by KCNJ16)alone can form a homotetramer and can form heterotetramers with Kir4.1(encoded by KCNJ10)or Kir4.2(encoded by KCNJ15).In most cases,homomeric Kir5.1 is non-functional,while hetero-meric Kir5.1 on the cell membrane contributes to the inward flow of K^(+)ions,which can be regulated by intracellular pH and a variety of signaling mechanisms.In the form of a heterotetramer,Kir5.1 regulates Kir4.1/4.2 activity and is involved in the maintenance of nephron function.Actually,homomeric Kir5.1 may also play a very important role in diseases,including in the ventilatory response to hypoxia and hypercapnia,hearing impairment,cardio-vascular disease and cancer.With an increase in the number of studies into the roles of Kir channels,researchers are paying more attention to the pathophysiological functions of Kir5.1.This minireview provides an overview regarding these Kir5.1 roles. | Junhui Zhang Jian Han Lingfei Li Qiong Zhang Yanhai Feng Youzhao Jiang Fang Deng Yuping Zhang Qinan Wu Bing Chen Jiongyu Hu | 2021 | Genes & Diseases2021，8，3： | 0 |
| 8 | How the electron-deficient Cp ligand facilitates Rh-catalyzed annulations with alkynes显示文摘The mechanism and origin of the Cp^(X) ligand effects on Rh-catalyzed annulations with alkynes were inves-tigated using DFT calculations and the approach of energy decomposition analysis(EDA).The results reveal that the alkyne migratory insertion is the rate-determining step for the reactions with both acetanilide and 2-phenyl-2-propanol substrates. | Han Gao Lingfei Hu Yanlei Hu Xiangying Lv Yan-BoWu Gang Lu | 2022 | Organic Chemistry Frontiers2022，9，4： | 0 |
| 9 | Origins of regio-and stereoselectivity in Cu-catalyzed alkyne difunctionalization with CO_(2) and organoboranes显示文摘The mechanism and origins of regio-and stereoselectivities of Cu-catalyzed alkyne difunctionalization with CO_(2) and trialkyl boranes were computationally investigated.The results show that the vicinal addition to alkyne is disfavored due to the kinetical inertness of the alkyl copper intermediate.The favored geminal addition proceeds through the cationic Cu-mediated stereoselective 1,2-migration pathway.The energy decomposition analysis indicates that theσ)(πPauli repulsion is the dominant factor for controlling the stereoselectivity. | Lingfei Hu Han Gao Yanlei Hu Xiangying Lv Yan-Bo Wu Gang Lu | 2022 | Organic Chemistry Frontiers2022，9，4： | 0 |
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