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您的检索式：作者名="Linbing Deng"
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| 1 | A Novel Chimeric Mitochondrial Gene Confers Cytoplasmic Effects on Seed Oil Content in Polyploid Rapeseed (Brassica napus)显示文摘Cytoplasmic effects (CEs) have been discovered to influence a diverse array of agronomic traits in crops, and understanding the underlying mechanisms can help accelerate breeding programs. Seed oil content (SOC) is of great agricultural, nutritional, and economic importance. However, the genetic basis of CEs on SOC (CE-SOC) remains enigmatic. In this study, we use an optimized approach to sequence the cytoplasmic (plastid and mitochondrial) genomes of allotetraploid oilseed rape (Brassica napus) cultivars, 51218 and 56366, that bear contrasting CE-SOC. By combining comparative genomics and genome-wide transcriptome analysis, we identify mitochondria-encoded orf188 as a potential CE-SOC determinant gene. Functional analyses in the model system Arabidopsis thaliana and rapeseed demonstrated that orf188 governs CE-SOC and could significantly increase SOC, strikingly, through promoting the yield of ATP. Consistent with this finding, transcriptional profiling with microarray and RNA sequencing revealed that orf188 affects transcriptional reprogramming of mitochondrial energy metabolism to facilitate ATP production. Intriguingly, orf188 is a previously uncharacterized chimeric gene, and the presence of this genetic novelty endows rapeseed with positive CE-SOC. Our results shed light on the molecular basis of CEs on a key quantitative trait in polyploid crops and enrich the theory of maternal control of oil content, providing new scientific guidance for breeding high-oil rapeseed germplasms. | Jun Liu Wanjun Hao Jing Liu Shihang Fan Wei Zhao Linbing Deng Xinfa Wang Zhiyong Hu Wei Hua Hanzhong Wang | 2019 | Molecular Plant2019，12，4： | 5 |
| 2 | Nano-silver-modified polyphosphazene nanoparticles with different morphologies:Design,synthesis,and evaluation of antibacterial activity显示文摘Drug-resistant bacteria present a severe threat to public health,emphasizing the importance of developing broad-spectrum antibacterial agents that are free from drug resistance.Among silver-based antibacterial agents,nano-silver has been found to exhibit the most promising and comprehensive performance.The exploration of the antibacterial capacity and morphological changes of silver nanoparticles(AgNPs)could offer a starting point for the development of safe and efficient antibacterial agents.In this study,three types of nano-silver-modified polyphosphazene(PRV)nanoparticles with different morphologies were synthesized using precipitation polymerization.These nanoparticles were characterized using various techniques,including Fourier-transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),scanning electron microscopy(SEM),transmission electron microscopy(TEM),and thermogravimetric analysis(TGA).The antibacterial activity of these nanoparticles against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)was assessed using minimum inhibitory concentration(MiC)/minimum bactericidal concentration(MBC)tests and inverted fluorescence microscopy.Our results revealed that the antibacterial activity of silver nanoparticles can vary significantly depending on their immobilized form.Ag@PRV Strawberry-like nanoparticles(NPs)exhibited higher antibacterial activity compared to Ag@PRV Yolk-Shell NPs and Ag@PRV Cable-like nanofibers(NFs).Notably,all three types of synthesized nanoparticles demonstrated a stronger bactericidal effect on Gram-positive bacteria than Gram-negative bacteria.Live/dead bacterial staining and scanning electron microscopy demonstrated that silver can kill bacteria by altering the permeability of their cell membranes.These findings offer valuable insights for designing and practically applying new silver-based antibacterial agents in the future. | Tairong Kuang Linbing Deng Sitao Shen Hongxia Deng Zhisen Shen Zhenjie Liu Zhengping Zhao Feng Chen Mingqiang Zhong | 2023 | Chinese Chemical Letters2023，34，12： | 0 |
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