| 1 | NACC-1 regulates hepatocellular carcinoma cell malignancy and is targeted by miR-760显示文摘Hepatocellular carcinoma(HCC)is the most prominent form of presentation in liver cancer.It is also the fourth most common cause of cancer-associated deaths globally.The role of nucleus accumbens associated protein-1(NACC-1)has been evaluated in several cancers.This protein is a transcriptional regulator that regulates a number of significant cellular processes.In the current study,we aimed to understand the role of NACC-1 in HCC.Primarily,we measured the expression of NACC-1 using quantitative real time polymerase chain reaction and western blot analysis.We knocked down the expression of NACC-1 in HCC cell lines Huh7 and HepG2 by transferring a commercially synthesized small interfering RNA and explored the impact of NACC-1 knockdown on cellular growth,migration,invasion,and chemoresistance to doxorubicin.Through bioinformatic analysis,we identified NACC-1 as a potential target of miR-760.Using a dual reporter luciferase assay,we confirmed the predicted target and assessed miR-760-mediated regulation of NACC-1 and rescue of tumorigenic phenotypes.We observed increased expression of NACC-1 in HCC.Furthermore,knockdown of NACC-1 resulted in reduced cell proliferation and invasion and increased susceptibility to doxorubicin-mediated chemosensitivity.Overexpression of miR-760 in HCC cell lines rescued NACC-1-mediated migration and invasion.We revealed that miR-760 regulated NACC-1 expression in HCC.Our data indicated that both miR-760 and NACC-1 could be used as prognostic markers,and miR-760 may have therapeutic benefits for HCC and other cancers. | Linan Yin Tingting Sun Ruibao Liu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，3： | 2 |
| 2 | Epigenetic modification associated with climate regulates betulin biosynthesis in birch显示文摘The Betula genus contains pentacyclic triterpenoid betulin known for its environmental adaptation and medicinal properties.However,the mechanisms underlying betulin biosynthesis responding to climate change remain unclear.In this study,the role of epigenetic modification(DNA methylation) in betulin biosynthesis was examined and how climatic factors influence it.Whole-genome bisulfite sequencing was performed for greenhouse-grown Chinese white birch(Betula platyphylla Sukaczev) treated with DNA methylation inhibitor zebularine(ZEB) and a natural birch population in Northeast China.ZEB treatment significantly affected the CHH methylation level of transposable elements and betulin content in a hormesis dose-dependent manner.The methylation and expression of bHLH9,a key transcriptional factor controlling betulin biosynthesis,were also consistently affected by ZEB treatment as a hormetic dose-response.In the natural population,there was a positive correlation between promoter methylation of bHLH9 and summer precipitation,while winter temperature was negatively correlated.Thus climate-dependent methylation of bHLH9 regulates the expression of downstream genes involved in betulin biosynthesis.This study highlights the role of environmental signals to induce epigenetic changes that result in betulin production,possibly helping to develop resilient plants to combat ongoing climate change and enhance secondary metabolite production. | Jiang Wang Bowei Chen Shahid Ali Tianxu Zhang Yu Wang He Zhang Lishan Wang Yonglan Zhang Linan Xie Tingbo Jiang Jing Yin Heike W.Sederoff Gaurav Zinta Ronald R.Sederoff Yuhua Li Qingzhu Zhang | 2023 | Journal of Forestry Research2023，34，1： | 0 |
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