| 1 | The chromosome-scale genome of Phoebe bournei reveals contrasting fates of terpene synthase (TPS)-a and TPS-b subfamilies显示文摘Terpenoids,including aromatic volatile monoterpenoids and sesquiterpenoids,function in defense against pathogens and herbivores.Phoebe trees are remarkable for their scented wood and decay resistance.Unlike other Lauraceae species investigated to date,Phoebe species predominantly accumulate sesquiterpenoids instead of monoterpenoids.Limited genomic data restrict the elucidation of terpenoid variation and functions.Here,we present a chromosome-scale genome assembly of a Lauraceae tree,Phoebe bournei,and identify 72 full-length terpene synthase(TPS)genes.Genome-level comparison shows pervasive lineage-specific duplication and contraction of TPS subfamilies,which have contributed to the extreme terpenoid variation within Lauraceae species.Although the TPS-a and TPS-b subfamilies were both expanded via tandem duplication in P.bournei,more TPS-a copies were retained and constitutively expressed,whereas more TPS-b copies were lost.The TPS-a genes on chromosome 8 functionally diverged to synthesize eight highly accumulated sesquiterpenes in P.bournei.The essential oil of P.bournei and its main component,b-caryophyllene,exhibited antifungal activities against the three most widespread canker pathogens of trees.The TPS-a and TPS-b subfamilies have experienced contrasting fates over the evolution of P.bournei.The abundant sesquiterpenoids produced by TPS-a proteins contribute to the excellent pathogen resistance of P.bournei trees.Overall,this study sheds light on the evolution and adaptation of terpenoids in Lauraceae and provides valuable resources for boosting plant immunity against pathogens in various trees and crops. | Xiao Han Junhong Zhang Shuang Han Sun Li Chong Guanliang Meng Minyan Song Yang Wang Shengcai Zhou Chengcheng Liu Luhuan Lou Xiongzhen Lou Longjun Cheng Erpei Lin Huahong Huang Qi Yang Zaikang Tong | 2022 | Plant Communications2022，3，6： | 3 |
| 2 | Cloning and Bioimformatic Analysis of Cellulose Synthase-like Protein Gene,CICsID1 from Cunninghamia lanceolata显示文摘The protein structure of the cellulose synthase-like protein(CSL) was similar to cellulose synthase(CesA),including the conservative sequence D,D,D,QXXRW.One full-length cDNA of the cellulose synthase-like protein D(CslD) gene was cloned by reverse transcriptase(RT)-polymerase chain reaction(PCR) with 5’,3’ rapid amplification of cDNA ends(RACE) methods using degenerate primers designed from the homologous sequences of the CesA genes.A multiple comparison sequence analysis was conducted concurrently with bioinformatic methods to analyze the obtained sequence.Results of the sequence analysis showed that this cDNA was 4 150 bp in length and contained a single open reading frame encoding a protein of 1 132 amino acids.The multiple comparison sequence analysis showed that the deduced amino acid sequence shared high similarity (over 71%) with the ClCslD genes from Populus tremuloides,Oryza sativa,and Arabidopsis thaliana. This work will help lay an important foundation for further molecular studies with cellulose synthesis of plants. | Peng Shasha Tong Zaikang Huang Huahong Zhou Houjun Shi Jian Lin Erpei | 2012 | Chinese Forestry Science and Technology2012，11，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员