| 1 | Intersected functional zone of transcriptional regulators patterns stemness within stem cell niche of root apical meristem显示文摘Root stem cell niche(SCN)consists of a quiescent center(QC)and surrounding stem cells.Disrupted symplastic communication leads to loss of stemness in the whole SCN.Several SCN regulators were reported to move between cells for SCN main-tenance.However,single mutant of these regulators is insu?cient to abolish QC stemness despite the high differentiation rate in surrounding stem cells.To dis-sect the mechanism behind such distinct stemness in SCN,we combined the mis-expression strategy with pWOX5:icals3m system in which QC is symplastically isolated.We found the starch accumulation in QC could be synergistically repressed by WUSCHEL-RE-LATED HOMEOBOX 5(WOX5),SHORT-ROOT(SHR),SCARCROW(SCR),and PLETHORA(PLT).Like PLTs,other core regulators also exhibited dimorphicfunctions by inhibiting differentiation at a higher dose while promoting cell division at a low protein level.Being located in the center of the intersected ex-pression zones,QC cells receive the highest level of core regulators,forming the most robust stemness within SCN.WUSCHEL-RELATED HOMEOBOX 5 was su?cient to activate PLT1/2 expression,contributing to the QC-enriched PLTs.Our results provide ex-perimental evidence supporting the long-standing hypothesis that the combination of spatial ex-pression,synergistic function and dosage effect of core regulators result in spatially distinct stemness in SCN. | Meizhi Xu Xu Gu Nengsong Liang Xinxin Bian Hong Wang Yaxin Qin Limin Pi Shuang Wu | 2020 | Journal of Integrative Plant Biology2020，62，7： | 1 |
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