| 1 | Comparative Proteomic Analysis of B. henselae Houston and B. henselae Marseille by Two-dimensional Gel Electrophoresis显示文摘Objective To compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis. Method Protein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis. Results Protein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 μg/μL and 2.200 μg/μL respectively. Sample protein of 40 μg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae. Conclusion The procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes. | SU-QING ZHAO*,# , YAN-FEI CAI?, AND ZHEN-YU ZHU,1 *Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France #Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090, Guangdong, China ?College of Resource and Environment, South China Agriculture University, Guangzhou 510642, Guangdong, China Department of Biochemistry, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou 510080, Guangdong, China | 2005 | Biomedical and Environmental Sciences2005，18，5： | 3 |
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