3篇
您的检索式：作者名="Liezhen Fu"
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| 1 | Construction of cDNA library, nucleotide sequence and analysis of entire genome of classical swine fever virus strain Shimen显示文摘According to the previously published CSFV sequences, 18 pairs of primers have been designed and synthesized, which cover the entire genome of CSFV strain Shimen. Each cDNA fragment has been amplified by RT-PCR from the anticoagulant blood of strain Shimen infected pig. The PCR products have been cloned respectively and sequenced. Results show that the cDNA library of strain Shimen and its nucleotide sequence have been obtained. The genomic RNA of strain Shimen is 12 298 nucleotides in length, containing a 5’ and a 3’ noncoding region 373 and 231 nt long respectively. The center of genome is a single large open reading frame of 11 697 nt which encodes a polyprotein of 3 898 amino acids. The entire sequence of strain Shimen has also been compared with that of other CSFV strains. | Qianhua Huang Chuyu Zhang Jiafu Wang Liezhen Fu Ning Wang Yan Zhu Jisen Huai Pengwei Zhang Jianshi Yu Hui Xu | 2000 | Chinese Science Bulletin2000，45，4： | 1 |
| 2 | Molecular Cloning and Nucleotide Sequence of the Attenuated Hog Cholera VirusLapinized Chinese Strain显示文摘The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt)including 374 nt and 239 nt at the 5’ and 3’-noncoding region, respectively. The complete genome sequence contained one large open reading frame which encoded an amino acid sequence of 3 898 residues with & calculated molecular weight of 437×103. Although there were mostly only small differences between the sequence of the HCLV strain and the published sequences of strains ALD, GPE-, Alfort and Brescia, there was one notable insertion of 12 nucleotides, TTTTCTTTTTTC in the 3’ non-coding region of HCLV strain. | Wang Jiafu Zhang Chuyu Fu Liezhen Wang Ning Huang Qianhua Zhang Pengwei(College of Life Sciences, Wuhan University, Wuhan 430072, China) | 1998 | Wuhan University Journal of Natural Sciences1998，3，4： | 1 |
| 3 | Molecular Cloning and NucleotideSequence of Classical SwineFever Virus Strain Shimen显示文摘According to the previously published CSFV sequences, 18 pairs of psrtially overlapping primers which span the entire genome of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was amplified by RT-PCR method from the anticoagulant blood of strain Shimen infected pig.The PCR fragments were cloned into PGEM-T vector respectively and sequenced. The results shoal that we have obtained the nucleotide sequence of strain Shimen. The viral RNA consists of 12 297 nucleotides including noncoding regions of 373 and 227 bases at the 5’and 3’ end, respectively, and a single large open reading frame spanning 11 697 nucleotides in the middle. tvhich encodes an amino acid sequence of 3 989 residues with a calculated molecular weight of 437. 6 × 103. The precisely sequencing of 5’and 3’ termini is undertaking. | Huang Qianhua Zhang Chuyu Wang Jiafu Wang Ning Fu Liezhen(College of Life Sciences, Wuhan University, Wuhan, 430072,China) | 1998 | Wuhan University Journal of Natural Sciences1998，3，4： | 1 |
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