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| 1 | Towards Kinetic Modeling of Global Metabolic Networks: Methylobacterium extorquens AM1 Growth as Validation显示文摘Here we report a systematic method for constructing a large scale kinetic metabolic model and its initial application to the modeling of central metabolism of Methylobacterium extorquens AM1, a methylotrophic and environmental important bacterium. Its central metabolic network includes formaldehyde metabolism, serine cycle, citric acid cycle, pentose phosphate pathway, gluconeogensis, PHB synthesis and acetyl-CoA conversion pathway, respiration and energy metabolism. Through a systematic and consistent procedure of finding a set of parameters in the physiological range we overcome an outstanding difficulty in large scale kinetic modeling: the requirement for a massive number of enzymatic reaction parameters. We are able to construct the kinetic model based on general biological considerations and incomplete experimental kinetic parameters. Our method consists of the following major steps: 1) using a generic enzymatic rate equation to reduce the number of enzymatic parameters to a minimum set while still preserving their characteristics; 2) using a set of steady state fluxes and metabolite concentrations in the physiological range as the expected output steady state fluxes and metabolite concentrations for the kinetic model to restrict the parametric space of enzymatic reactions; 3) choosing enzyme constants K's and K'eqs optimized for reactions under physiological concentrations, if their experimental values are unknown; 4) for models which do not cover the entire metabolic network of the organisms, designing a dynamical exchange for the coupling between the metabolism represented in the model and the rest not included. | Ping Ao Lik Wee Lee Mary E. Lidstrom Lan Yin Xiaomei Zhu | 2008 | 生物工程学报2008，24，6： | 10 |
| 2 | The Role of Synthetic Biology in Atmospheric Greenhouse Gas Reduction: Prospects and Challenges显示文摘The long atmospheric residence time of CO2 creates an urgent need to add atmospheric carbon drawdown to CO2 regulatory strategies.Synthetic and systems biology(SSB),which enables manipulation of cellular phenotypes,offers a powerful approach to amplifying and adding new possibilities to current land management practices aimed at reducing atmospheric carbon.The participants(in attendance:Christina Agapakis,George Annas,Adam Arkin,George Church,Robert Cook-Deegan,Charles DeLisi,Dan Drell,Sheldon Glashow,Steve Hamburg,Henry Jacoby,Henry Kelly,Mark Kon,Todd Kuiken,Mary Lidstrom,Mike MacCracken,June Medford,Jerry Melillo,Ron Milo,Pilar Ossorio,Ari Patrinos,Keith Paustian,Kristala Jones Prather,Kent Redford,David Resnik,John Reilly,Richard J.Roberts,Daniel Segre,Susan Solomon,Elizabeth Strychalski,Chris Voigt,Dominic Woolf,Stan Wullschleger,and Xiaohan Yang)identified a range of possibilities by which SSB might help reduce greenhouse gas concentrations and which might also contribute to environmental sustainability and adaptation.These include,among other possibilities,engineering plants to convert CO2 produced by respiration into a stable carbonate,designing plants with an increased root-to-shoot ratio,and creating plants with the ability to self-fertilize.A number of serious ecological and societal challenges must,however,be confronted and resolved before any such application can be fully assessed,realized,and deployed. | Charles DeLisi Aristides Patrinos Michael MacCracken Dan Drell George Annas Adam Arkin George Church Robert Cook-Deegan Henry Jacoby Mary Lidstrom Jerry Melillo Ron Milo Keith Paustian John Reilly Richard J.Roberts Daniel Segrè Susan Solomon Dominic Woolf Stan D.Wullschleger Xiaohan Yang | 2020 | BioDesign Research2020，，1： | 2 |
| 3 | Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria显示文摘 | Marx C J Lidstrom M E | 2002 | Biotechniques2002，33，5： | 1 |
| 4 | In vitro studies on periodontal ligament cells and enamel matrix derivative显示文摘 | Gestrelius S Andersson C Lidstrom D | 1997 | J Clin Periodontol1997，24，92： | 1 |
| 5 | Microwave assisted organic synthesis-a review 显示文摘 | Lidstrom P Tierney J Wathey B | 2001 | Tetrahedron2001，57，45： | 1 |
| 6 | Fractures of the distal end of the radius显示文摘 | | 1959 | Acta Orthop Scand1959，，： | 1 |
| 7 | In vitro studies on periodontal ligament cells and enamel matrix derivative 显示文摘 | Gestrelius S Andersson C Lidstrom D | 1997 | J Clin Periodontal1997，24，9： | 1 |
| 8 | Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related显示文摘 | Costello A Lidstrom M E | 1995 | FEMS Microbiol Lett1995，132，3： | 1 |
| 9 | In vitro studies on periodontal ligament cells and enamel matrix derivative 显示文摘 | Gestrelius S Andersson C Lidstrom D | 1997 | J Clin Periodontol1997，24，92： | 1 |
| 10 | In vitro studies on periodontal ligament cells and enamel matrix derivative 显示文摘 | Gestrelius S Andersson C Lidstrom D | 1997 | J Clin Periodontol1997，24，92： | 1 |
| 11 | Microwave assisted organic synthesis-a review显示文摘 | Lidstrom P Tierney J Wathey B | 2001 | Tetrahedron2001，57，： | 1 |
| 12 | Occurrence and environmental behavior of he bactericide triclosan and its methyl and derivative in surface waters and in wasterwater显示文摘 | Buerge I J Pooiger T | 2002 | Environ Sci Technol2002，36，： | 1 |
| 13 | Microwave Assisted Organic Synthesis--A Review显示文摘 | Pelle Lidstrom Jason Tierney Bernard Wathay | 2001 | Tetrohedron2001，57，： | 1 |
| 14 | In vitro studies on periodontal ligament cells and EMD显示文摘 | Gestrelius S Andersson C Lidstrom D | 1997 | J Clin Periodontol1997，24，2： | 1 |
| 15 | In vitro studies onperiodontal ligament cells and enamel matrix derivative 显示文摘 | Gestrelius S Andersson C Lidstrom D | 1997 | J Clin Periodontal1997，24，92： | 1 |
| 16 | Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related显示文摘 | Andrew J Holmes Andria Costello Mary E Lidstrom | 1995 | FEMS Microbiology Letters1995，132，： | 1 |
| 17 | Microwave as- sisted organic synthesis-A review 显示文摘 | Lidstrom P Tierney J Wathey B | 2001 | Tetrahedron2001，57，45： | 1 |
| 18 | Life-on-a-chip显示文摘 | LIDSTROM M E MELDRUM D R | 2003 | Nature Reviews2003，1，2： | 1 |
| 19 | Microwave assisted organic synthesis-a review显示文摘 | Lidstrom P Tierney J Wathey B | 2001 | Tetrahedron2001，57，45： | 1 |
| 20 | Fractures of the distal radius:A clinical and sta tistical study of end results显示文摘 | Lidstrom A | 1959 | Acta Orthop Scand Suppl1959，41，： | 1 |
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