3篇
您的检索式：作者名="Libing QIU"
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| 1 | Identification and Expression Profile of a Neuropeptide LFRFamide-Like Gene During Different Stages of Gonadal Development in the Cephalopod Sepia pharaonis显示文摘Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function of neuropeptide LFRFamide in Sepia pharaonis,the full-length cDNA of LFRFamide-like gene(named SpLFRFL,MG869822.1)was identified with rapid amplification of cDNA ends(RACE)method.The sequence of SpLFRFL was 860 bp in length and encoded 188 amino acids containing 4 different mature peptides:1 copy of PHTPFRFamide,NSLFRFamide,TIFRFamide,and 3 copies of GNLFRFamide.Multiple alignment and phylogenetic analysis results showed that SpLFRFL shared high identity with LFRFamides of Sepia officinalis and Sepiella japonica and had the closest relationship with them.Through quantitative Real-time PCR(qRT-PCR),it was found that the SpLFRFL gene was highly expressed in the optic lobe and brain at three different stages during gonad development in both genders.Moreover,the four mature peptides at a concentration of 0.01μmol L^(−1) could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1(CHOK1)induced by SpGnRH.These data suggest that SpLFRFL might be involved in the development and reproduction of S.pharaonis.The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming. | QIU Jiayin SUN Lianlian LI Shuang ZHOU Xu CHI Changfeng ZHENG Libing | 2024 | Journal of Ocean University of China2024，23，2： | 0 |
| 2 | Technique of Earthworms Restoring Soil in Greenhouse Cultivation显示文摘The production environment of greenhouse cultivation is relatively closed,the multiple cropping index is high,the management of fertilizationwatering and pesticideapplication isblindtosomeextent,andthe phenomenonofcontinuous cropping isalsocommonSoilquali-ty affects the sustainable development of greenhouse cultivation.Earthworm is a ubiquitous invertebrate organism in soil,an important part of soil system,a link between terrestrial organisms and soil organisms,an important link in the small cycle of soil material organisms,and plays an important role in maintaining the structure and function of soil ecosystem.Different ecotypes of earthworms are closely related to their habi-tats(soil layers)and food resource preferences,and then affect their ecological functions.The principle of earthworm regulating soil function is essentially the close connection and interaction between earthworm and soil microorganism.Using different ecotypes of earthworms and bio-logical agents to carry out combined remediation of greenhouse cultivation soil is a technical model to realize sustainable development of green-house cultivation. | Yanjiao LI Jiafei SU Zhiu ZHANG Guang QI Jianhua CHEN Lixuan KOU Limin WANG Wenxian LIU Junyi ZHANG Libing QIU | 2024 | Asian Agricultural Research2024，16，1： | 0 |
| 3 | Potential anticancer activity analysis of piscidin 5-like from Larimichthys crocea显示文摘Antitumor activity is one characteristic function of some certain antimicrobial peptides(AMPs)found in recent years.In the present study,we attempted to detect potential anticancer activity of a recombinant piscidin 5-like from Larimichthys crocea(r Lc-P5L)which owned widely antibacterial and strong antiparasitic activity in vitro.The light microscope observation indicated r Lc-P5L was of antitumor activity to He La cells,293 T cells and L929 cells.MTT assay showed the toxic sensitivity of r Lc-P5L to three tumor cell strains was 293 T>L929>He La.Scanning electron microscope(SEM)results showed r Lc-P5L behaved like a lytic peptide to cause damage on cells membrane of L929 cells by forming globular clusters,even pores at 60μmol/L,or degrading membrane to make it completely lose cytoskeleton structure at 80μmol/L;r Lc-P5L treatment also resulted in DNA degradation.Fluorescence observation results indicated r Lc-P5L could cause L929 cells at least two obvious changes:one is nucleus,nuclear chromatin condensed in the margin,nuclear volume became smaller and shrank to be out of shape,or lysed to be debris;the other is cytoskeleton,they became disordered and polarized to make cells atrophic shapes,or even lysed to be debris.In summary,r Lc-P5L owned potential anticancer activity causing membrane structure damage and genome DNA degradation.Interestingly,treatment with different concentration of r Lc-P5L seemingly caused the similar but different changes,whether it indeed gave rise to cancer cells diverse death way,the further studies should be performed,and the detailed mechanisms were still need further explored. | Libing Zheng Jiayin Qiu Huihui Liu Changfeng Chi Longshan Lin | 2022 | Acta Oceanologica Sinica2022，41，3： | 0 |
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