2篇
您的检索式：作者名="Liangqing Fu"
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| 1 | Knockdown of STAT3 by shRNA inhibits the growth of CAOV3 ovarian cancer cell line in vitro and in vivo显示文摘抄写 3 的组成地激活的信号变换器和使活跃之物(STAT3 ) 包括卵巢的癌症在许多肿瘤的形成起一个重要作用。在这研究,对 STAT3 特定的 RNA 干扰被采用在 STAT3 发信号的抑制上并且在卵巢的癌症 CAOV3 房间的生长上学习它的效果。对 STAT3 和爬的 shRNA 控制表示特定的小发卡 RNA (shRNA ) 的质粒向量 pGenesil-1-GFP-U6 被构造。在进 CAOV3 房间的转染以后, STAT3 shRNA 明确地在 mRNA 和蛋白质层次压制了 STAT3 表示。同时, Bcl-xL, cyclin D1,和 c-myc 的表情是下面调整的,而劈开的 caspase 3 起来调整。另外,在 CAOV3 细胞的击倒的禁止的抛锚无关的生长和导致的 apoptosis,和在裸体老鼠的减少的肿瘤生长与卵巢的癌症细胞植入的 STAT3。 | Feng Huang Xiaoyun Tong Liangqing Fu Ronghua Zhang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，6： | 18 |
| 2 | Inhibition of the Antigen-Induced Activation of RBL-2H3 Cells by Gab2 siRNA显示文摘Gab2 plays an important role in FcεRI mediated signal events which lead to degranulation from mast cells. The present study was designed to investigate the effect of the synthetic Gab2 (scaffolding adapter Grb2-associated binder 2) siRNA on the antigen-induced activation of RBL-2H3 cells. A double stranded siRNA against Gab2- mRNA was synthesized and transfected into RBL-2H3 cells. After 6 h, cells were then sensitized with dinitrophenyl (DNP)-specific IgE overnight and challenged with dinitrophenyl-human serum albumin (DNP-HSA) to induce mast cell degranulation before supernatants were collected. Effects of Gab2 siRNA on antigen-induced release of β-hexosaminidase and histamine, cytokine production and regulation of the proteins in the pathway were measured by enzymatic assay, EIA, ELISA and Western blotting. Treatment with Gab2 siRNA significantly decreased Gab2 expression, inhibited the FcεRI-mediated mast cell release of β-hexosaminidase and histamine, reduced the production of IL-4 and TNF-α and inhibited the phosphorylation of Akt, PKCδ and p38 mitogen-activated protein kinase (MAPK). Data showed that Gab2 siRNA could suppress the antigen-induced activation of RBL-2H3 cells and suggested a possible mechanism through inhibition of signaling molecules downstream of Gab2 in the FcεRI-mediated Ca2+-independent pathway. Furthermore, potential usefulness of Gab2 knock-down as a method for inhibition of mast cell-mediated allergic reactions was demonstrated. | Feng Huang Xiaoyun Tong Huaming Deng Liangqing Fu Ronghua Zhang | 2008 | Cellular & Molecular Immunology2008，5，6： | 1 |
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