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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Effects of Chinese herbal polysaccharidds on the immunity and growth performance of young broilers显示文摘 | CHEN H L LID F CHANG B Y | 2003 | Poult Sci2003，82，3： | 1 |
| 3 | Multiattribute Decision Making Models and Methods Using Intuitionistic Fuzzy Sets显示文摘 | LID F | 2005 | Journal of computer and System Sciences2005，70，1： | 1 |
| 4 | Concept of virtual electric/magnetic walls and its realization with artificial magnetic conductor technique显示文摘 | LID C BOONE F BOZZI M | 2008 | IEEE Microwave and Wireless Components Letters2008，18，11： | 1 |
| 5 | An edge embedded marker-based watershed algorithm for high spatial resolution remote sensing image segmentation显示文摘 | LID ZHANG G F WU Z C | 2010 | IEEE Trans Image Process2010，19，10： | 1 |
| 6 | Microstructural Models of Dynamic Recrystallization in Hot-Deformed Inconel 625 Superalloy 显示文摘 | GUO Q M LID F GUO S L | 2012 | Materials and Manufacturing Processes2012，9，27： | 1 |
| 7 | Pyrrolizidine alkaloids from Trollius laxus显示文摘 | Lid - dellJ R Stermitz F R | 1996 | Chemical Abstracts1996，，： | 1 |
| 8 | PMRD: plant microRNA database显示文摘 | ZhangZ H Yu J Y LiD F etal | 2010 | Nucleic Acids Research2010，3，： | 1 |
| 9 | Bubble formation in an annular granularJet dispersed by a central air roundJet显示文摘 | LU H LID H F LI W F XUJ L | 2013 | AIChEJ2013，59，6： | 1 |
| 10 | Complete statistical characterization of discrete-Time LQG and cumulant control 显示文摘 | QIAN F C GAO J J LID | 2012 | IEEE Transactions on Automatic Control2012，57，8： | 1 |
| 11 | Study on the radialJet velocity distribution of two closely spaced opposedJets显示文摘 | SUN Z G LI W F LID H F | 2009 | IntJ Heat Fluid Flow2009，30，6： | 1 |
| 12 | Dietary supplementation with zinc oxide increases IGF- I and IGF- I receptor gene expression in the small intestine of weanling piglets 显示文摘 | LI X L YIN J D LID F | 2006 | The Journal of Nutrition2006，136，7： | 1 |
| 13 | Effects of emulsificauion, fat encapsulation, and pelleting on weanling pig performance and nutrient digestibility 显示文摘 | XING J J HEUGTEN E LID F | 2004 | J Anim Sci2004，82，： | 1 |
| 14 | Experimental and numerical study on stagnation point offset of turbulent opposedJets显示文摘 | LI W SUN Z LID H WANG F YU Z | 2008 | Chern EngJ2008，138，13： | 1 |
| 15 | Transient hy persensitivity to soybean meal in the early weaned pig显示文摘 | LID F Nelssen J L Reddy P G | 1990 | Journal of Animal Science1990，，68： | 1 |
| 16 | Habitat evaluation of wild Amur tiger (Panthera tigris altaica) and conservation priority setting in north-eastern China 显示文摘 | LUAN X F QU Y LID Q | 2011 | Journal of Environmental Management2011，92，1： | 1 |
| 17 | A novel pH-sensitive (+)-a- tocopherol-5-fluorouracil adduct with antioxidant and anticancer properties显示文摘 | LID W TIAN F F GE Y S | 2011 | Chem Commun2011，47，10： | 1 |
| 18 | Dietary supplementation with zinc oxide decreases expression of the stem cell factor in the small intestine of weanling pigs 显示文摘 | OU D Y LID F CAO Y H | 2007 | The Journal of Nutritional Biochemistry2007，18，12： | 1 |
| 19 | Effect of fat sources and combinations on starter pig performance,nutrient digestibility and intestinal morphology显示文摘 | LID F THALER R C NELSSEN J L | 1990 | Journal of Animal Science1990，68，： | 1 |
| 20 | Transient hypersensitivity to soybean meal in the early weaned pig 显示文摘 | LID F NELSSEN J L REDDY P G | 1990 | Journal of Animal Science1990，68，： | 1 |
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