|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Curcumin inhibits the replication of enterovirus 71 in vitro显示文摘Human enterovirus 71(EV71)is the main causative pathogen of hand,foot,and mouth disease(HFMD)in children.The epidemic of HFMD has been a public health problem in Asia-Pacific region for decades,and no vaccine and effective antiviral medicine are available.Curcumin has been used as a traditional medicine for centuries to treat a diversity of disorders including viral infections.In this study,we demonstrated that curcumin showed potent antiviral effect again EV71.In Vero cells infected with EV71,the addition of curcumin significantly suppressed the synthesis of viral RNA,the expression of viral protein,and the overall production of viral progeny.Similar with the previous reports,curcumin reduced the production of ROS induced by viral infection.However,the antioxidant property of curcumin did not contribute to its antiviral activity,since N-acetyl-L-cysteine,the potent antioxidant failed to suppress viral replication.This study also showed that extracellular signal-regulated kinase(ERK)was activated by either viral infection or curcumin treatment,but the activated ERK did not interfere with the antiviral effect of curcumin,indicating ERK is not involved in the antiviral mechanism of curcumin.Unlike the previous reports that curcumin inhibited protein degradation through ubiquitin–proteasome system(UPS),we found that curcumin had no impact on UPS in control cells.However,curcumin did reduce the activity of proteasomes which was increased by viral infection.In addition,the accumulation of the short-lived proteins,p53 and p21,was increased by the treatment of curcumin in EV71-infected cells.We further probed the antiviral mechanism of curcumin by examining the expression of GBF1 and PI4KB,both of which are required for the formation of viral replication complex.We found that curcumin significantly reduced the level of both proteins.Moreover,the decreased expression of either GBF1 or PI4KB by the application of siRNAs was sufficient to suppress viral replication.We also demonstrated that curcumin showed anti-apoptotic activity at the early stage of viral infection.The results of this study provide solid evidence that curcumin has potent anti-EV71 activity.Whether or not the down-regulated GBF1 and PI4KB by curcumin contribute to its antiviral effect needs further studies. | Ying Qin Lexun Lin Yang Chen Shuo Wu Xiaoning Si Heng Wu Xia Zhai Yan Wang Lei Tong Bo Pan Xiaoyan Zhong Tianying Wang Wenran Zhao Zhaohua Zhong | 2014 | Acta Pharmaceutica Sinica B2014，4，4： | 5 |
| 2 | Stress Granule Formation is One of the Early Antiviral Mechanisms for Host Cells Against Coxsackievirus B Infection显示文摘Stress granules(SGs) are intracellular granules formed when cellular translation is blocked and have been reported to be involved in a variety of viral infections. Our previous studies revealed that SGs are involved in the coxsackievirus B(CVB)infection process, but the role of SGs in CVB infection has not been fully explored. In this study, we found that CVB type 3(CVB3) could induce SG formation in the early phase of infection. Results showed that levels of CVB3 RNA and protein were significantly inhibited during the early stage of CVB3 infection by the elevated formation of SGs, while viral RNA and protein synthesis were significantly promoted when SG formation was blocked. Our findings suggest that SG formation is one of the early antiviral mechanisms for host cells against CVB infection. | Xia Zhai Shuo Wu Lexun Lin Tianying Wang Xiaoyan Zhong Yang Chen Weizhen Xu Lei Tong Yan Wang Wenran Zhao Zhaohua Zhong | 2018 | Virologica Sinica2018，33，4： | 2 |
| 3 | Expression Profile and Function Analysis of Long Non-coding RNAs in the Infection of Coxsackievirus B3显示文摘The roles of IncRNAs in the infection of enteroviruses have been barely demonstrated.In this study,we used coxsackievirus B3(CVB3),a typical enterovirus,as a model to investigate the expression profiles and functional roles of IncRNAs in enterovirus infection.We profiled IncRNAs and mRNA expression in CVB3-infected HeLa cells by IncRNA-mRNA integrated microarrays.As a result,700 differentially expressed IncRNAs(431 up-regulated and 269 down-regulated)and 665 differentially expressed mRNAs(299 up-regulated and 366 down-regulated)were identified in CVB3 infection.Then we performed IncRNA-mRNA integrated pathway analysis to identify potential functional impacts of the differentially expressed mRNAs,in which IncRNA-mRNA correlation network was built.According to IncRNA-mRNA correlation,we found that XLOC-001188,an IncRNA down-regulated in CVB3 infection,was negatively correlated with NFAT5 mRNA,an anti-CVB3 gene reported previously.This interaction was supported by qPCR detection following siRNA-mediated knockdown of XLOC-001188,which showed an increase of NFAT5 mRNA and a reduction of CVB3 genomic RNA.In addition,we observed that four most significantly altered IncRNAs,SNHG11,RP11-145F16.2,RP11-1023L17.1 and RP11-1021N1.2 share several common correlated genes critical for CVB3 infection,such as BRE and IRF2BP1.In all,our studies reveal the alteration of IncRNA expression in CVB3 infection and its potential influence on CVB3 replication,providing useful information for future studies of enterovirus infection. | Lei Tong Ye Qiu Hui Wang Yunyue Qu Yuanbo Zhao Lexun Lin Yan Wang Weizhen Xu Wenran Zhao Hongyan He Guangze Zhao Mary HZhang Decheng Yang Xingyi Ge Zhaohua Zhong | 2019 | Virologica Sinica2019，34，6： | 2 |
| 4 | Characterization of the glyceraldehyde - 3 - phosphate dehydrogenase ( GAPDH ) gene from the halotolerant 'alga dunaliella salina and inhibition of its expression by RNAi显示文摘 | Jia Yanlong Xue Lexun Liu Hongtao | 2009 | Curr Microbiol2009，58，5： | 1 |
| 5 | Tetrahydropalmatine attenuates liver fibrosis by suppressing endoplasmic reticulum stress in hepatic stellate cells显示文摘To the Editor: Liver fibrosis was characterized by the activation of hepatic stellate cells (HSCs) and extracellular matrix deposition. The pathogenesis of liver fibrosis involved many aspects. Studies have found that endoplasmic reticulum stress (ERS) in HSCs could lead to HSC activation and aggravate liver fibrosis.[1] Although liver fibrosis has been extensively studied, effective anti-fibrotic drugs are lacking at present. Tetrahydropalmatine (THP) was the active ingredient of Chinese herbs Corydalis yanhusuo. Previous studies have found the hepatoprotective effect of THP. Our study aimed to investigate the effects of THP on liver fibrosis and the underlying mechanisms. | Yuanyuan Xuan Shiyun Chen Xin Ding Lexun Wang Shengwen Li Guizhi Yang Tian Lan | 2022 | Chinese Medical Journal2022，，5： | 1 |
| 6 | Autocrine Motility Factor Receptor Signaling Pathway Promotes Cell Invasion via Activation of ROCK-2 in Esophageal Squamous Cell Cancer Cells显示文摘 | Lili Wang Guiqin Hou Lexun Xue Jie Li Pan Wei Peirong Xu | 2010 | Cancer Investigation2010，，10： | 1 |
| 7 | Increased expression of transgene in stably transformed cells of Dunaliella salina by matrix attachment regions 显示文摘 | Wang Tianyun Xue Lexun Hou Weihong | 2007 | Appl Microbiol Biotechnol2007，76，： | 1 |
| 8 | Increased expression of transgene in stably transformed cells of Dunaliella salina by matrix attachment regions显示文摘 | Tianyun Wang Lexun Xue Weihong Hou Baosheng Yang Yurong Chai Xiang Ji Yafeng Wang | 2007 | Applied Microbiology and Biotechnology2007，，3： | 1 |
| 9 | Preparation of recombinant human canstatin using transgenic Dunaliella safina显示文摘 | Shuying Feng Sanqiang Li Qinghua Li Ke Shi Lexun Xue | 2014 | Acta Biochimica et Biophysica Sinica2014，46，5： | 0 |
| 10 | CCAAT/Enhancer-Binding Proteins in Fibrosis:Complex Roles Beyond Conventional Understanding显示文摘CCAAT/enhancer-binding proteins(C/EBPs)are a family of at least six identified transcription factors that contain a highly conserved basic leucine zipper domain and interact selectively with duplex DNA to regulate target gene expression.C/EBPs play important roles in various physiological processes,and their abnormal function can lead to various diseases.Recently,accumulating evidence has demonstrated that aberrant C/EBP expression or activity is closely associated with the onset and progression of fibrosis in several organs and tissues.During fibrosis,various C/EBPs can exert distinct functions in the same organ,while the same C/EBP can exert distinct functions in different organs.Modulating C/EBP expression or activity could regulate various molecular processes to alleviate fibrosis in multiple organs;therefore,novel C/EBPs-based therapeutic methods for treating fibrosis have attracted considerable attention.In this review,we will explore the features of C/EBPs and their critical functions in fibrosis in order to highlight new avenues for the development of novel therapies targeting C/EBPs. | Lexun Wang Jiaojiao Feng Yanyue Deng Qianqian Yang Quxing Wei Dewei Ye Xianglu Rong Jiao Guo | 2023 | Research2023，，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员