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您的检索式：作者名="Lester Brewer"
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| 1 | Genotyping-by-sequencing of pear (Pyrus spp.) accessions unravels novel patterns of genetic diversity and selection footprints显示文摘Understanding of genetic diversity and marker-trait relationships in pears(Pyrus spp.)forms an important part of gene conservation and cultivar breeding.Accessions of Asian and European pear species,and interspecific hybrids were planted in a common garden experiment.Genotyping-by-sequencing(GBS)was used to genotype 214 accessions,which were also phenotyped for fruit quality traits.A combination of selection scans and association analyses were used to identify signatures of selection.Patterns of genetic diversity,population structure and introgression were also investigated.About 15000 high-quality SNP markers were identified from the GBS data,of which 25%and 11%harboured private alleles for European and Asian species,respectively.Bayesian clustering analysis suggested negligible gene flow,resulting in highly significant population differentiation(Fst=0.45)between Asian and European pears.Interspecific hybrids displayed an average of 55%and 45%introgression from their Asian and European ancestors,respectively.Phenotypic(firmness,acidity,shape and so on)variation between accessions was significantly associated with genetic differentiation.Allele frequencies at large-effect SNP loci were significantly different between genetic groups,suggesting footprints of directional selection.Selection scan analyses identified over 20 outlier SNP loci with substantial statistical support,likely to be subject to directional selection or closely linked to loci under selection. | Satish Kumar Chris Kirk Cecilia Deng Claudia Wiedow Mareike Knaebel Lester Brewer | 2017 | Horticulture Research2017，4，1： | 8 |
| 2 | Genome mapping of postzygotic hybrid necrosis in an interspecific pear population显示文摘Deleterious epistatic interactions in plant inter-and intraspecific hybrids can cause a phenomenon known as hybrid necrosis,characterized by a typical seedling phenotype whose main distinguishing features are dwarfism,tissue necrosis and in some cases lethality.Identification of the chromosome regions associated with this type of incompatibility is important not only to increase our understanding of the evolutionary diversification that led to speciation but also for breeding purposes.Development of molecular markers linked to the lethal genes will allow breeders to avoid incompatible inbred combinations that could affect the expression of important agronomic tratis co-segregating with these genes.Although hybrid necrosis has been reported in several plant taxa,including Rosaceae species,this phenomenon has not been described previously in pear.In the interspecific pear population resulting from a cross between PEAR3(Pyrus bretschneideri×Pyrus communis)and‘Moonglow’(P.communis),we observed two types of hybrid necrosis,expressed at different stages of plant development.Using a combination of previously mapped and newly developed genetic markers,we identified three chromosome regions associated with these two types of lethality,which were genetically independent.One type resulted from a negative epistatic interaction between a locus on linkage group 5(LG5)of PEAR3 and a locus on LG1 of‘Moonglow’,while the second type was due to a gene that maps to LG2 of PEAR3 and which either acts alone or more probably interacts with another gene of unknown location inherited from‘Moonglow’. | Sara Montanari Lester Brewer Robert Lamberts Riccardo Velasco Mickael Malnoy Laure Perchepied Philippe Guérif Charles-Eric Durel Vincent G M Bus Susan E Gardiner David Chagné | 2016 | Horticulture Research2016，3，1： | 1 |
| 3 | Fine-mapping and validation of the genomic region underpinning pear red skin colour显示文摘Red skin colour is an important target trait in various pear breeding programmes.In this study,the genetic control of red skin colour was investigated in an interspecific population derived using the descendants of the red sport European pear cultivar‘Max Red Bartlett’(MRB)and the red-blushed Chinese pear cultivar‘Huobali’.Approximately 550 seedlings from nine families were phenotyped for red skin over-colour coverage(Ocolcov)and the intensity of red over-colour(Ocolint)on a 0–9 scale,and genotyped using genotyping-by-sequencing.Genome-wide association analyses were conducted using 7500 high-quality single nucleotide polymorphisms(SNPs).Genomic regions on linkage groups(LG)4 and 5 were found to be associated,and the best SNP(S578_25116)on LG4 accounted for~15%of phenotypic variation in Ocolcov and Ocolint.The association of S578_25116 with Ocolcov and Ocolint was successfully validated in a sample of~200 European and Asian pear accessions.The association with red skin at locus S578_25116 was not present in Asian pear accessions,suggesting its close proximity to the MRB’s Cardinal gene.Several putative candidate genes,including MYB transcription factors(PCP027962 and PCP027967),were identified in the quantitative trait locus region on LG4 and await functional validation. | Satish Kumar Chris Kirk Cecilia Hong Deng Claudia Wiedow Mengfan Qin Richard Espley Jun Wu Lester Brewer | 2019 | Horticulture Research2019，6，1： | 1 |
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