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| 1 | 生命价值:无需回避评估显示文摘治理环境污染的主要效益是健康效益,即降低发病率和死亡率,但治理污染要消耗稀缺资源(经济成本)。在涉及污染与健康的环境费用效益分析中,社会必须权衡经济收入与生命价值。估价生命价值有4种方法:人力资本法、工资隐含价格法、防护费用法和实验评价法。可以从不同途径评估中国人生命价值。 | Jin Leshan(China Agricultural University,Beijing 100094) | 1999 | 重庆环境科学1999，21，4： | 10 |
| 2 | Study on the drug resistance and the binding mode of HIV-1 integrase with LCA inhibitor显示文摘Human immunodeficiency virus type 1 (HIV-1) integrase (IN) is an essential enzyme in the lifecycle of this virus and also an important target for the study of anti-HIV drugs. The binding mode of the wild type IN core domain and its G140S mutant with L-Chicoric acid (LCA) inhibitor were investigated by using multiple conformation molecular docking and molecular dynamics (MD) simulation. Based on the binding modes, the drug resistance mechanism was explored for the G140S mutant of IN with LCA. The results indicate that the binding site of the G140S mutant of IN core domain with LCA is different from that of the core domain of the wild type IN, which leads to the partial loss of inhibition potency of LCA. The flexibility of the IN functional loop region and the interactions between Mg2+ ion and the three key residues (i.e., D64, D116, E152) stimulate the biological operation of IN. The drug resistance also lies in several other important effects, such as the repulsion between LCA and E152 in the G140S mutant core domain, the weakening of K159 binding with LCA and Y143 pointing to the pocket of the G140S mutant. All of the above simulation results agree well with experimental data, which provide us with some helpful information for designing the drug of anti-HIV based on the structure of IN. | HU JianPing1,2, CHANG Shan1, CHEN WeiZu1 & WANG CunXin1 1 College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, China 2 Department of Chemistry & Life Sciences, Leshan Normal University, Leshan 614004, China | 2007 | Science China Chemistry2007，50，5： | 7 |
| 3 | Determining antimicrobial resistance profiles and identifying novel mutations of Neisseria gonorrhoeae genomes obtained by multiplexed MinION sequencing显示文摘Gonorrhea is one of the most common sexually transmitted diseases worldwide. To cure infection and prevent transmission,timely and appropriate antimicrobial therapy is necessary. Unfortunately, Neisseria gonorrhoeae, the etiological agent of gonorrhea, has acquired nearly all known mechanisms of antimicrobial resistance(AMR), thereby compromising the efficacy of antimicrobial therapy. Treatment failure resulting from AMR has become a global public health concern. Whole-genome sequencing is an effective method to determine the AMR characteristics of N. gonorrhoeae. Compared with next-generation sequencing, the MinION sequencer(Oxford Nanopore Technologies(ONT)) has the advantages of long read length and portability. Based on a pilot study using MinION to sequence the genome of N. gonorrhoeae, we optimized the workflow of sequencing and data analysis in the current study. Here we sequenced nine isolates within one flow cell using a multiplexed sequencing strategy. After hybrid assembly with Illumina reads, nine integral circular chromosomes were obtained. By using the online tool Pathogenwatch and a BLAST-based workflow, we acquired complete AMR profiles related to seven classes of antibiotics. We also evaluated the performance of ONT-only assemblies. Most AMR determinants identified by ONT-only assemblies were the same as those identified by hybrid assemblies. Moreover, one of the nine assemblies indicated a potentially novel antimicrobial-related mutation located in mtrR which results in a frame-shift, premature stop codon, and truncated peptide.In addition, this is the first study using the MinION sequencer to obtain complete genome sequences of N. gonorrhoeae strains which are epidemic in China. This study shows that complete genome sequences and antimicrobial characteristics of N.gonorrhoeae can be obtained using the MinION sequencer in a simple and cost-effective manner, with hardly any knowledge of bioinformatics required. More importantly, this strategy provides us with a potential approach to discover new AMR determinants. | Chi Zhang Feng Wang Cansheng Zhu Leshan Xiu Yamei Li Li Li Bo Liu Yizhun Li Yaling Zeng Boyang Guo Junping Peng | 2020 | Science China(Life Sciences)2020，63，7： | 2 |
| 4 | Evaluation of ceU-free fetal DNA as a second-trimester matenlal serum marker of Down syndrome pregnancy 显示文摘 | Farina A LeShane ES Lambert-Messerlian GM | 2003 | Clin Chem2003，49，2： | 1 |
| 5 | Maternal serum cell-free fetal DNA levels are increased in cases of trisomy 13 but not trisomy 18显示文摘 | Wataganara T LeShane ES Farina A | 2003 | Hum Genet2003，112，2： | 1 |
| 6 | Maternal serum cell-free fetal DNA levels are increased in cases of trisomy 13 but not trisomy 18显示文摘 | Wataganara T LeShane ES Farina A | 2003 | Hum Genet2003，112，2： | 1 |
| 7 | Maternal serum cellfree fetal DNA levels are increased in cases of trisomy 13 but not trisomy 18 显示文摘 | Wataganara T LeShane ES Farina A | 2003 | Hum Genet2003，112，2： | 1 |
| 8 | Lep-tin action through hypothalamic nitric oxide synthase-1-ex-pressing neurons controls energy balance显示文摘 | Leshan RL Greenwald-Yarnell M Patterson CM | | 0，，5： | 1 |
| 9 | Cell-flee fetal DNA levels in maternal plasma after elective first-trimester termination of pregnancy显示文摘 | Wataganara T Chen A Y LeShane E S | 2004 | FertilSteril2004，81，3： | 1 |
| 10 | Two-stage elevation of cellfree fetal DNA in maternal sera before onset of preeclampsia 显示文摘 | Levine R J Qian C Leshane ES | 2004 | Am J Obstet Gynecol2004，190，3： | 1 |
| 11 | High levels of fetal cellfree DNA in maternal serum: a risk factor for spontaneous preterm delivery 显示文摘 | Farina A LeShane ES Romero R | 2005 | Am J Obstet Gynecol2005，193，42： | 1 |
| 12 | Fetal cell-free plasma DNA concentrations in maternal blood are stable 24 hours after collection analysis of first and third-trimester samples显示文摘 | Angert R M LeShane E S Lo Y M Chan L Y Delli-Bovi L C Bianehi D W | 2003 | Clin Chem2003，49，： | 1 |
| 13 | Water use in agriculture in China: importance, challenges, and implications for policy显示文摘 | Leshan Jin Warren Young | 2001 | Water Policy2001，，3： | 1 |
| 14 | Circulating cell-free fetal nucleic acid analysis may be a novel marker of fetomatemal hemorrhage after elective first-trimester termination of pregnancy 显示文摘 | Wataganara T Leshane ES Chen AY | 2004 | Ann N Y Acad Sci2004，1022，： | 1 |
| 15 | Mice lacking inhibitory leptin receptor signals are lean with normal endocrine function显示文摘 | Bjomhohn M Munzberg H Leshan RL | 2007 | J Clin Invest2007，117，5： | 1 |
| 16 | High levels of fetal cell-free DNA in maternal serum: a risk factor for spontaneous preterm delivery 显示文摘 | Farina A LeShane ES Romero R | 2005 | Am J Obstet Gynecol2005，193，2： | 1 |
| 17 | Leptinreceptor signaling and action in the central nervous system显示文摘 | Leshan RL Bjornholm M Munzberg H | 2006 | Obesity2006，，： | 1 |
| 18 | Cell- free fetal DNA in the cerebrospinal fluid of women during tbe peripartum period显示文摘 | Angert RM Leshane ES Yamell R W | 2004 | Am 1 Obstet Gynecol2004，190，4： | 1 |
| 19 | Cell free fetal DNA levels in maternal plasma after elective first-trimester termination of pregnancy显示文摘 | Wataganara T Chen AY LeShane ES | 2004 | Fertil Steril2004，81，3： | 1 |
| 20 | Mice lacking inhibitory leptin receptor signals are lean with normal endocrine function显示文摘 | Bjornholm M Münzberg H Leshan RL Villanueva EC Bates SH Louis GW Jones JC Ishida-Takahashi R Bjorbaek C Myers MG Jr | | 0，，： | 1 |
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