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| 1 | Reaction kinetics of CaC2 formation from powder and compressed feeds显示文摘 | Renxing Wang Zhenyu Liu Leiming Ji Xiaojin Guo Xi Lin Junfei Wu Qingya Liu | 2016 | Frontiers of Chemical Science and Engineering2016，10，4： | 6 |
| 2 | Using Interactome Big Data to Crack Genetic Mysteries and Enhance Future Crop Breeding显示文摘The functional genes underlying phenotypic variation and their interactions represent“genetic mysteries”.Understanding and utilizing these genetic mysteries are key solutions for mitigating the current threats to agriculture posed by population growth and individual food preferences.Due to advances in highthroughput multi-omics technologies,we are stepping into an Interactome Big Data era that is certain to revolutionize genetic research.In this article,we provide a brief overview of current strategies to explore genetic mysteries.We then introduce the methods for constructing and analyzing the Interactome Big Data and summarize currently available interactome resources.Next,we discuss how Interactome Big Data can be used as a versatile tool to dissect genetic mysteries.We propose an integrated strategy that could revolutionize genetic research by combining Interactome Big Data with machine learning,which involves mining information hidden in Big Data to identify the genetic models or networks that control various traits,and also provide a detailed procedure for systematic dissection of genetic mysteries,Finally,we discuss three promising future breeding strategies utilizing the Interactome Big Data to improve crop yields and quality. | Leiming Wu Linqian Han Qing Li Guoying Wang Hongwei Zhang Lin Li | 2021 | Molecular Plant2021，14，1： | 2 |
| 3 | Effects of typical algae species(Aphanizomenon flosaquae and Microcystis aeruginosa) on photoreduction of Hg2+ in water body显示文摘Photoreduction characteristics of divalent inorganic mercury (Hg2+) in the presence of specific algae species are still not well known.Laboratory experiments were conducted in the present study to identify the effects of different concentrations of living/dead algae species,including Aphanizomenon flosaquae (AF) and Microcystis aeruginosa (MA),on the photoreduction rate of Hg2+ under various light conditions.The experimental results showed that percentage reduction of Hg2+ was significantly influenced by radiation wavelengths,and dramatically decreased with the presence of algae.The highest percentage reduction of Hg2+ was induced by UV-A,followed by UV-B,visible light and dark for both living and dead AF,and the order was dark > UV-A > UV-B > visible light for both living and dead MA.There were two aspects,i.e.,energy and attenuation rate of light radiation and excrementitious generated from algae metabolisms,were involved in the processes of Hg2+ photoreduction with the presence of algae under different light conditions.The percentage reduction of Hg2+ decreased from 15% to 11% when living and dead AF concentrations increased by 10 times (from 106 to 105 cells/mL),and decreased from11% to ~9% in the case of living and dead MA increased.Algae can adsorb Hg2+ and decrease the concentration of free Hg2+,thus inhibiting Hg2+ photoreduction,especially under the conditions with high concentrations of algae.No significant differences were found in percentage reduction of Hg2+ between living and dead treatments of algae species.The results are of great importance for understanding the role of algae in Hg2+ photoreduction. | Rongguo Sun Yafei Mo Xinbin Feng Leiming Zhang Lin Jin Qiuhua Li | 2019 | Journal of Environmental Sciences2019，31，11： | 2 |
| 4 | An Early Paleozoic Tectonic Mélange at the Western Margin of West Cathaysia: Constraints from Organic-walled Microfossils显示文摘The Jiangshan-Shaoxing-Pingxiang Fault(JSP Fault) is traditionally considered as the boundary between the Yangtze and Cathaysia blocks in South China. Whether the previously defined Shenshan and Kuli formations located along the JSP fault and near the Xinyu City, Jiangxi Province, are continuous strata or parts of a tectonic mélange is important for understanding the geological history of South China. A carbonaceous phyllite from the area, previously considered as part of the Neoproterozoic Shenshan and Kuli formations, is analyzed palynologically in this study. The AsteridiumComasphaeridium acritarch assemblage found in the slate can be correlated with the basal Cambrian AsteridiumHeliosphaeridium-Comasphaeridium(AHC) acritarch assemblage in Tarim and the Yangtze Block. The early Cambrian biostratigraphical age assignment for the carbonaceous phyllite indicates the presence of both Neoproterozoic and Cambrian rocks in the sedimentary package, and supports that the package is a part of tectonic mélange rather than a continuous Neoproterozoic strata. The Cambrian slate is the youngest known lithology in the mélange at present. | WANG Lijun ZHANG Kexin HE Weihong YIN Leiming Shoufa LIN | 2020 | Acta Geologica Sinica(English Edition)2020，94，4： | 2 |
| 5 | Impact of PM 2.5 chemical compositions on aerosol light scattering in Guangzhou — the largest megacity in South China显示文摘 | Jun Tao Leiming Zhang Kinfai Ho Renjian Zhang Zejian Lin Zhisheng Zhang Mang Lin Junji Cao Suixing Liu Gehui Wang | 2014 | Atmospheric Research2014，，： | 1 |
| 6 | Prediction of high frequency resistance in polymer electrolyte membrane fuel cells using long short term memory based model显示文摘High-frequency resistance(HFR)is a critical quantity strongly related to a fuel cell system’s performance.It is beneficial to estimate the fuel cell system’s HFR from the measurable operating conditions without resorting to costly HFR measurement devices.In this study,we propose a data-driven approach for a real-time prediction of HFR.Specifically,we use a long short-term memory(LSTM)based machine learning model that takes into account both the current and past states of the fuel cell,as characterized through a set of sensors.These sensor signals form the input to the LSTM.The data is experimentally collected from a vehicle lab that operates a 100 kW automotive fuel cell stack running on an automotive-scale test station.Our current results indicate that our prediction model achieves high accuracy HFR predictions and outperforms other frequently used regression models.We also study the effect of the extracted features generated by our LSTM model.Our study finds that only very few dimensions of the extracted feature are influential in HFR prediction.The study highlights the potential to monitor HFR condition accurately and timely on a car. | Tong Lin Leiming Hu Willetta Wisely Xin Gu Jun Cai Shawn Litster Levent Burak Kara | 2021 | Energy and AI2021，3，1： | 0 |
| 7 | Characteristics of phosphorus chemistry and its geographical distribution in显示文摘The study on the phosphorus element chemistry in plants,geographical distributions,outputs and inputs of phosphorus from the soils by 61 plants in the Haihe River valley, North China, are presented in this paper. | Jiang Gaoming Huang Yinxiao Lin Shunhua Han Rongzhuang Gao Leiming (Institute of Botany,Chinese Academy of Sciences, Beijing 100044,China) | 1995 | Journal of Environmental Sciences1995，7，1： | 0 |
| 8 | QTL Mapping for Plant Height of Wheat under Different Irrigation Modes显示文摘In order to mine gene loci associated with drought resistance and water use efficiency,and provide guidance for the breeding of drought-resistant wheat varieties,quantitative trait loci(QTLs)for plant height(PH)and stem height(SH)of wheat were evaluated using a population of 302 recombinant inbred lines(RILs)derived from a cross between drought-tolerant cultivar Luohan 2 and water-sensitive cultivar Weimai 8 under different water regimes in six environments.Totally 17 additive QTLs for PH and SH were identified and distributed on chromosomes 2B,3A,3B,4A,5A,5D,6B,7A and 7B,respectively,which explained3.3%to 26.95%phenotypic variations,with additive effect of 1.919 6 to 5.382 8 cm.Among them,14 QTLs,24 times and 5 QTLs,7 times were detected in three irrigation environments and three drought stress environments,respectively.Both the number of QTLs for PH and the times detected in drought stress environments were significantly less than those in irrigation environments.Of all the 17 QTLs for PH,12 were detected only in irrigation environment,three QTLs were detected only in drought stress environment,and two QTLs were detected in both environments,suggesting that the expression of the genes is greatly influenced by environmental condition.QPh-WL-7A.3 detected between maker Xbarc049 and Xgwm273 on chromosome 7A in all the three drought stress environments,can enhance2.481 5-3.697 2 cm of PH,explain 8.6%-10.0%additive effect,so it is useful for the genetic improvement of drought tolerance and molecular marker-assisted selection in wheat. | Yanyun REN Ling HUANG Minmin SHAO Leiming SUN Kai ZHAO Jinping WANG Xingke XU Weiying FENG Jifeng WANG Lu YAN Shichong WANG Lin WANG | 2017 | Agricultural Biotechnology2017，6，4： | 0 |
| 9 | Construction and expression of hepatitis B virus vector encoding TC-tagged core protein显示文摘Virus tagged with greenfluorescent protein(GFP)contributes to the visualization and study of the virus in living cells.However,the hepatitis B virus(HBV)particle,which is a compact virion with limited internal space,cannot be incorporated with GFP tag as a large fragment.It was recently reported that protein genetically inserted with a smaller size tetracysteine(TC)tag could be specially labeled by a biarsenicalfluorescent dye in living cells.In this study,we constructed a recombinant HBV vector encoding TC-tagged core protein for biarsenical labeling of HBV virion.TC tag was genetically inserted near the immunodominant c/e1 site of HBV core protein by mutagenesis.Western blot and enzyme-linked immu-nosorbent assay(ELISA)analysis showed that the TC-tagged core protein,hepatitis B surface antigen(HBsAg)and hepatitis B e antigen(HBeAg)could be expressed in cells transfected with the recombinant HBV vector,which is similar to the cells transfected with wild-type HBV vector.Reverse transcription-polymerase chain reaction(RT-PCR)and Southern blot analysis showed that HBV virion formation was affected by the genetic insertion of TC tag into core protein in some degree,but cells transfected with the HBV vector could still produce HBV virions incorporated with TC-tagged core proteins.Taken together,the recombinant HBV vector can serve as a useful tool to produce HBV virions incorporated with TC-tagged core proteins to befluorescently labeled by biarsencial dye for visualizing and studying HBV in living cells. | Yuanyuan LIN Xiaoming CHENG Yuhu SONG Li ZHOU Peiyuan LI Ying CHANG Leiming XU Jinjian YAO Jusheng LIN | 2009 | Frontiers of Medicine2009，3，4： | 0 |
| 10 | Effect of Irrigation and Fertilization on Population Structure and Yield of Wheat显示文摘[Objectives] This study was conducted to investigate the effects of irrigation and fertilization on population structure and yield of wheat.[Methods]With Shannong 29 as an experimental material,the effects of irrigation and fertilization on population,dry matter accumulation and yield of wheat were studied.[Results]Integrated water-saving irrigation and fertilization of ridged field was the best with the highest population,dry matter accumulation and yield of wheat.[Conclusions]This study provides a theoretical basis for high-yield and high-efficiency wheat production with saved water and fertilizers. | Minmin SHAO Leiming SUN Xingke XU Kai ZHAO Ling HUANG Weiying FENG Jifeng WANG Lu YAN Lin WANG | 2020 | Agricultural Biotechnology2020，9，2： | 0 |
| 11 | Effects of Different Line Spacing and Seedling Belt Width on Yield Formation of Broad-Width Fine Sowing Wheat显示文摘In order to explore the interaction effects of line spacing and seedling belt width on wheat yield formation and improve the cultivation techniques of broadwidth and fine seeding of wheat,a high-yielding winter wheat cultivar Shannong 28 was selected as material. Using the split plot design,the main plot was set with line spacing as 20,25 and 30 cm,respectively,and the sub-plot was set with seedling belt width as 3,5,7,9 and 11 cm,respectively. Then,the population dynamics,dry matter accumulation and translocation and yield of wheat were studied under the experimental conditions. The results showed that under the line spacing of 20 cm,the dry matter accumulation and yield of winter wheat were higher with the seedling belt width of 5 cm. When the line spacing was 25 cm,the dry matter accumulation and yield under the seedling belt width of 9 cm reached a high level. Under the line spacing of 30 cm,Shannong 28 achieved higher dry matter accumulation and yield with the seedling belt width of 11 cm. Comprehensive analysis revealed that the suitable treatment for Shannong 28 was 25 cm of line spacing with 9 cm of seedling belt width,which could realize the coordination of the three factors of yield composition and get higher yield. Therefore,the reasonable line spacing and seedling belt width were the important technical ways to realize high yield of wheat. | Minmin SHAO Leiming SUN Kai ZHAO Xingke XU Ling HUANG Lin WANG Lu YAN Zhengchun JU | 2020 | Agricultural Biotechnology2020，9，3： | 0 |
| 12 | A cost-effective ts CUT&Tag method for profiling transcription factor binding landscape显示文摘Knowledge of the transcription factor binding landscape(TFBL)is necessary to analyze gene regulatory networks for important agronomic traits.However,a low-cost and high-throughput in vivo chromatin profiling method is still lacking in plants.Here,we developed a transient and simplified cleavage under targets and tagmentation(tsCUT&Tag)that combines transient expression of transcription factor proteins in protoplasts with a simplified CUT&Tag without nucleus extraction.Our tsCUT&Tag method provided higher data quality and signal resolution with lower sequencing depth compared with traditional ChIP-seq.Furthermore,we developed a strategy combining tsCUT&Tag with machine learning,which has great potential for profiling the TFBL across plant development. | Leiming Wu Zi Luo Yanni Shi Yizhe Jiang Ruonan Li Xinxin Miao Fang Yang Qing Li Han Zhao Jiquan Xue Shutu Xu Tifu Zhang Lin Li | 2022 | Journal of Integrative Plant Biology2022，64，11： | 0 |
| 13 | Anaerobic oxidation of arsenite by bioreduced nontronite显示文摘The redox state of arsenic controls its toxicity and mobility in the subsurface environment. Understanding the redox reactions of arsenic is particularly important for addressing its environmental behavior. Clay minerals are commonly found in soils and sediments, which are an important host for arsenic. However, limited information is known about the redox reactions between arsenic and structural Fe in clay minerals. In this study, the redox reactions between As(Ⅲ)/As(Ⅴ) and structural Fe in nontronite NAu-2 were investigated in anaerobic batch experiments. No oxidation of As(Ⅲ) was observed by the native Fe(Ⅲ)-NAu-2. Interestingly, anaerobic oxidation of As(Ⅲ) to As(Ⅴ) occurred after Fe(Ⅲ)-NAu-2 was bioreduced. Furthermore, anaerobic oxidization of As(Ⅲ) by bioreduced NAu-2 was significantly promoted by increasing Fe(Ⅲ)-NAu-2 reduction extent and initial As(Ⅲ) concentrations. Bioreduction of Fe(Ⅲ)-NAu-2 generated reactive Fe(Ⅲ)-O-Fe(Ⅱ) moieties at clay mineral edge sites. Anaerobic oxidation of As(Ⅲ) was attributed to the strong oxidation activity of the structural Fe(Ⅲ) within the Fe(Ⅲ)-O-Fe(Ⅱ) moieties. Our results provide a potential explanation for the presence of As(Ⅴ) in the anaerobic subsurface environment. Our findings also highlight that clay minerals can play an important role in controlling the redox state of arsenic in the natural environment. | Ziwang Zhao Ying Meng Yahua Wang Leiming Lin Fuyu Xie Fubo Luan | 2021 | Journal of Environmental Sciences2021，33，12： | 0 |
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