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| 1 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 2 | Depot-specific differences in adipose tissue gene expression in lean and obese subjects显示文摘 | LEFEBVRE A M LAVILLE M VEGA N | 1998 | Diabetes1998，47，1： | 1 |
| 3 | Contribution of ovarian and uterine color Doppler in medically assisted reproduction techniques (ART)显示文摘 | Ardaens Y Gougeon A Lefebvre C | 2002 | Gynecol Obstet Fertil2002，30，9： | 1 |
| 4 | Evolution of precipitate microstructures during the retrogression and re-ageing heat treatment of an A1-Zn-Mg-Cu alloy显示文摘 | MARLAUD T DESCHAMPS A BLEY F LEFEBVRE W BAROUX B | 2010 | Acta Materialia2010，58，14： | 1 |
| 5 | Physical and sensory properties of model foods supplemented with cauliflower fiber显示文摘 | Femenia A Lefebvre C Thebaudin Y | 1997 | Journal of Food Science1997，62，4： | 1 |
| 6 | Spray characteristics of aerated liquid pressure atomizers 显示文摘 | Lefebvre A H Wang X F Martin C A | 1988 | AIAA J Prop Power1988，4，4： | 1 |
| 7 | Modeling nitrogen cycling in a mariculture ecosystem as a tool to evaluate its outflow显示文摘 | BACHER C MEURET A | 2001 | Estuarine Coastal and Shelf Science2001，52，： | 1 |
| 8 | Weak Extinction Limits of Turbulence Heterogeneous Fuel/Air Mixtures显示文摘 | Lefebvre A H | 1980 | Transactions of ASME Journal of Engineering for Power1980，102，： | 1 |
| 9 | Syphilis in Nantes tertiary care hospital between 2000 and 2010:a case series of 36 hospitalized patients显示文摘 | Lefebvre M Biron C Guillouzouic A | 2013 | Rev Med Interne2013，34，9： | 1 |
| 10 | Vastus medialis: anatomical and functional considerations and implications based upon human and cadaveric studies显示文摘 | Lefebvre R Leroux A Poumarat G | 2006 | J Manipulative Physiol Ther2006，29，2： | 1 |
| 11 | A novel method of atomization with potentialgas turbine application显示文摘 | Lefebvre A H | 1988 | Indian Defence Sci1988，38，： | 1 |
| 12 | Surface organometallic chemistry of tin reactivity of tetraalkyltin complexes and tributyltin hydride toward silica显示文摘 | Théolier A Lefebvre F | 1993 | J Am Chem Soc1993，115，2： | 1 |
| 13 | Nonalcoholic steatohepatitis 显示文摘 | Luyckx FH Scheen A J Lefebvre PJ | 2003 | Lancet2003，361，9367： | 1 |
| 14 | A design procedure for effervescent atomizers显示文摘 | Chin J S Lefebvre A H | 1995 | Journal of Engineering for Gas Turbines andPower1995，117，2： | 1 |
| 15 | Contribution of ovarian and uterine color Doppler in medically assisted reproduction techniques (ART) 显示文摘 | Ardaens Y Gougeon A Lefebvre C | 2002 | Gynecol Obstet Fertil2002，30，9： | 1 |
| 16 | Determinants and Impacts of Environmental Performance in SMEs显示文摘 | Lefebvre E Lefebvre L A Talbot S | 2003 | R&D Management2003，33，： | 1 |
| 17 | Influence of flow conditions on deposits fron~ heated hydrocarbon fuels 显示文摘 | / Chin J S Lefebvre A H | 1993 | Journal of Engineering for Gas Turbines] / and Power1993，115，： | 1 |
| 18 | Surface organometallic chemistry of tin: formation of the grafted organometallic complex (≡Si-O)2Sn(n-C4H9)2 by reaction of H2Sn(n-C4H9)2 with the surface of partially dehydroxylated silica显示文摘 | Choplin A Lefebvre F | 1994 | Inorg Chem1994，33，6： | 1 |
| 19 | Anti-sperm antibodies are not associated with sperm DNA damage: a prospective study of infertile men 显示文摘 | Zini A Phillips S Lefebvre J | 2010 | J Reprod Immunol2010，8，2： | 1 |
| 20 | Glucocorticoids induce the expression of the leptin gene through a non-classical mechanism of transcriptional activation显示文摘 | Devos P Lefebvre A M Shrivo I | 1998 | Eur J Biochem1998，253，： | 1 |
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