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| 1 | Permeabilization of Escherichia coli with ampicillin for a whole cell biocatalyst with enhanced glutamate decarboxylase activity显示文摘The activity of whole-cell biocatalysts is strongly compromised by the cell envelope, which is a permeability barrier against the diffusion of substrates and products. Although common chemical or physical permeabilization methods used in cultured cells enhance cell permeability, these methods inevitably add several extra processing steps after cell cultivation, as well as impede large scale processing. To increase membrane permeability and cellbound glutamate decarboxylase(GAD) activity of recombinant Escherichia coli(BL21(DE3)-p ET28a-gad B) cells without the need for an additional permeabilization step, we investigated the permeabilizing effects of adding cell wall synthesis inhibitors or surfactants to the culture media. Ampicillin was the most effective at improving cell-bound GAD activity of the BL21(DE3)-p ET28a-gad B, although it decreased the cell biomass yield. The best permeabilization effect was observed using an ampicillin concentration of 5 μg·ml-1. Using this concentration,the cell biomass did decrease by 40.58%, but the cell-bound GAD activity of BL21(DE3)-p ET28a-gad B and total cell-bound GAD activity per milliliter of culture was enhanced by 6.24- and 3.64-fold, respectively. Treatment of BL21(DE3)-p ET28a-gad B cells with 5 μg·ml-1ampicillin resulted in structural changes to the cell envelope,but did not substantially affect GAD expression. By entrapping the ampicillin-treated cells in an open pore gelation matrix, which is a polymer derived from polyvinyl alcohol(PVA), alginate, and boric acid, the transformation rate of γ-aminobutyric acid(GABA) at the 10 th cycle produced by immobilized and permeabilized cells remained 46% of the first cycle. GAD activity of the immobilized, permeabilized cells remained over 90% after30 days of storage at 4 °C. | Weirui Zhao Sheng Hu Jun Huang Piyu Ke Shanjing Yao Yinlin Lei Lehe Mei Jinbo Wang | 2016 | Chinese Journal of Chemical Engineering2016，24，7： | 2 |
| 2 | Insular gliomas: the case for surgical management 显示文摘 | Simon M Neuloh G yon Lehe M | 2009 | J Nenrosnrg2009，110，4： | 1 |
| 3 | Diffusion coefficients in intrahollow calcium alginate microcapsules显示文摘 | Chai Yi Mei Lehe Lin Dongqiang | 2004 | J Chem Eng Data2004，49，： | 1 |
| 4 | Focal cortical dysplasia: long term seizure outcome after surgical treatment 显示文摘 | Kral T yon Lehe M Podlogar M | 2007 | J Neurol Neurosurg Psychiatry2007，78，8： | 1 |
| 5 | Gliomas of the cingulate gyrus: surgical management and functional outcome 显示文摘 | Von Lehe M Schramm J | 2009 | Neurosurg Focus2009，27，： | 1 |
| 6 | The effect of cryogenic grinding and hammer milling on the flavour quality of ground pepper ( Piper nigrum L.)显示文摘 | Hong Liu Fankui Zeng Qinghuang Wang Shiyi Ou Lehe Tan Fenglin Gu | 2013 | Food Chemistry2013，，4： | 1 |
| 7 | Epilepsy surgery of the cin- gulate gyrus and the frontomesial cortex显示文摘 | von Lehe M Wagner J Wellmer J | 2012 | Neurosurgery2012，70，： | 1 |
| 8 | Simple and complex dysembryoplastic neuroepithelial tumors (DNT) va- riants: clinical profile, MRI and histopathology 显示文摘 | Campos AR Clusmann H yon Lehe M | 2009 | Neuro- radiol2009，51，7： | 1 |
| 9 | Doxorubicin downregulates cell surface BT-H1 expression and upregulates its nuclear expression in breast cancer cells:role of B7-H1 as an anti-apoptotic molecule 显示文摘 | Ghebeh H Lehe C Barhoush E | 2010 | Breast Cancer Res2010，12，4： | 1 |
| 10 | Magnetically Recoverable Core–Shell Nanocomposites with Enhanced Photocatalytic Activity显示文摘 | MiaomiaoYe QiaoZhang YongxingHu JianpingGe ZhendaLu LeHe ZhonglinChen YadongYin | 2010 | Chemistry – A European Journal2010，，21： | 1 |
| 11 | Skin irritants and contact sensitizers induce Langerhans cell migration and maturation at irritant concentration显示文摘 | Jacobs J J Lehe C L Hasegawa H | 2006 | Exp Dermatol2006，15，6： | 1 |
| 12 | Biochemical and morphologic characterization of acrylamide peripheral neuropathy显示文摘 | Lehing E J Persaud A Dyer K R | 1998 | Toxicol Appl Pharmacol1998，151，： | 1 |
| 13 | Surgical treatment of occipital lobe epilepsy 显示文摘 | Binder DK Von Lehe M Kral T | 2008 | J Neurosurg2008，109，1： | 1 |
| 14 | Tumor recurrence and malignant progression of gangliogliomas显示文摘 | Majores M yon Lehe M Fassunke J | 2008 | Cancer2008，113，12： | 1 |
| 15 | Glioma-related seizures: glutamateis the key 显示文摘 | Simon M von Lehe M | 2011 | Nat Med2011，17，10： | 1 |
| 16 | Skin irritants and contact sensitizers induce Langerhans cell migration and maturation at irritant concentration显示文摘 | Jacobs J J Lehe'C L Hasegawa H | 2006 | Exp Dermatol2006，15，6： | 1 |
| 17 | Comparison of cocoa beans from china, indonesia and papua new guinea显示文摘 | GU Fenglin TAN Lehe WU Huasong | 2013 | Foods2013，2，2： | 1 |
| 18 | Processing and Structural Properties of Waxy Maize Starch Nanocrystals Reinforced Natural Rubber显示文摘 | He lehe Angellier Sonia Molina-Boisseau Laurent Lebrun | 2005 | Macromolecules2005，38，： | 1 |
| 19 | Surgical Treatment of Occipital Lobe Epilepsy显示文摘 | Binder D K Von Lehe M Kral T | 2008 | J Neurosurg2008，109，1： | 1 |
| 20 | Equilibrium saltation:mass fluxes,aerodynamic entrainment,and dependence on grain prop-erties显示文摘 | DOORSCHOT J J LEHING M | 2002 | Boundary-Layer Meteorology2002，104，： | 1 |
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