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| 1 | CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses显示文摘AIM: To investigate the influence of the CagA diversity in Helicobacter pylori (H. pylori ) strains from Colombia on the host cell biology. METHODS: Eighty-four H. pylori-cagA positive strains with different Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs patterns, isolated from patients with gastritis (n=17), atrophic gastritis (n=17), duodenal ulcer (n=16), intestinal metaplasia (n=16) and gastric cancer (n=18), were included. To determine the integrity of the cag pathogenicity island (cag PAI) we evaluated the presence of cagA, cagT, cagE, and cag10 genes by polymerase chain reaction. AGS gastric epithelial cellswere infected with each strain and assayed for translo-cation and tyrosine phosphorylation of CagA by western blot, secretion of interleukin-8 (IL-8) by enzyme-linked immuno sorbent assay after taking supernatants from cocultures and cell elongation induction. For cell elongation quantification, coculture photographs were taken and the proportion of 'hummingbird' cells (>15 μm) was determined. RESULTS: Overall 72% (60/84) of the strains were found to harbor a functional cag PAI. Levels of phos-phorylated CagA were significantly higher for isolates from duodenal ulcer than the ones in strains from gas-tritis, atrophic gastritis, intestinal metaplasia and gastric cancer (49.1% ± 23.1% vs 21.1% ± 19.5%, P < 0.02; 49.1% ± 23.1% vs 26.2%±14.8%, P<0.045; 49.1% ± 23.1% vs 21.5% ± 19.5%, P<0.043 and 49.1% ± 23.1% vs 29.5% ± 27.1%, P < 0.047 respectively). We observed variable IL-8 expression levels ranging from 0 to 810 pg/mL and from 8.8 to 1442 pg/mL at 6 h and 30 h post-infection, respectively. cagPAI-defective strains did not induce detectable levels of IL-8 at 6 h post-infection. At 30 h post-infection all strains induced IL-8 expression in AGS cells, although cagPAI-defective strains induced significantly lower levels of IL-8 than strains with a functional cagPAI (57.1 ± 56.6 pg/mL vs 513.6 ± 338.6 pg/mL,P < 0.0001). We did not observe differences in the extent of cell elongation induction between strains with a functional or a defective cagPAI in 6 h cocultures. At 24 h post infection strains with functionalcagPAI showed high diversity in the extent of hummingbird phenotype induction ranging from 7% to 34%. cag PAI defective strains induced significantly lower levels of elongation than strains with functional cag-PAI with one or more than one EPIYA-C motif (15.1% ± 5.2%vs 18.9% ± 4.7%,P < 0.03; and 15.1% ± 5.2% vs 20.0% ± 5.1%, P < 0.003 respectively). No differences were observed in cellular elongation inductionor IL-8 expression among H. pylori strains bearing one and more than one EPIYA-C motifs, neither at 6 h nor at 24 h of coculture. There were no associations between the levels of induction of cell elongation or IL-8 expression and number of EPIYA motifs or pathology. CONCLUSION: The present work describes a lack of association between H. pylori CagA protein EPIYA motifs variations from Colombian isolates and disease-associated cellular responses. | Carlos Alberto Fajardo Andrés Javier Quiroga Andrea Coronado Karen Labrador Nicole Acosta Pilar Delgado Carlos Jaramillo María Mercedes Bravo | 2013 | World Journal of Gastrointestinal Oncology2013，5，3： | 3 |
| 2 | Classifying DNA Methylation Imbalance Data in Cancer Risk Prediction Using SMOTE and Tomek Link Methods显示文摘 | Chao Liu Jia Wu Labrador Mirador Yang Song Weiyan Hou | 2018 | 国际计算机前沿大会会议论文集2018，，2： | 2 |
| 3 | Progression of carotid intima-media thickness and plasma antioxidants : The Los Angeles Atherosclerosis Study 显示文摘 | Dwyer JH Paul- Labrador M J Fan J | 2004 | Arteriosclerosis Thrombosis and Vascular Biology2004，24，2： | 1 |
| 4 | Psychological changes accompanying and mediating stress-management training for essential hypertension显示文摘 | Sanz J Labrador FJ | 1998 | Appl Psychophysiol Biofeedback1998，23，3： | 1 |
| 5 | Antihis- tone and anti - double - stranded deoxyribonucleic acid antibodies are associated with renal disease in systemic lupus erythematosus 显示文摘 | Cortes - Hernandez J Ordi - Ros J Labrador M | 2004 | Am J Med2004，116，3： | 1 |
| 6 | Prediction of NaCl, nitrate and nitrite contents in minced meat by using a voltammetric electronic tongue and an impedimetric sensor显示文摘 | Roberto H. Labrador Rafael Masot Miguel Alca?iz Diana Baigts Juan Soto Ramón Martínez-Ma?ez Eduardo García-Breijo Luis Gil José M. Barat | 2010 | Food Chemistry2010，，3： | 1 |
| 7 | Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion显示文摘 | Hu H Li M Labrador JP | 2005 | Proc Natl Acad Sci U S A2005，102，12： | 1 |
| 8 | Polymyositis/derrnatomyosistis-associated lung disease: analysis of a series of 81 patients显示文摘 | Selve OA Labrador HM Munoz GX | 2005 | Lupus2005，14，7： | 1 |
| 9 | Oral Magnesium Therapy Improves Endothelial Function in Patients With Coronary Artery Disease显示文摘 | Shechter M Shark M Labrador MJP | 2000 | Circulation2000，102，： | 1 |
| 10 | A survey on human activity recognition using wearable sensors 显示文摘 | Lara O D Labrador M A | 2013 | IEEE Communications Surveys & Tu- torials2013，15，3： | 1 |
| 11 | Antihistone and anti-double-stranded deoxyribonucleic acid antibodies are as- sociated with renal disease in systemic lupus erythematosus显示文摘 | Cortfis-Herndndez J Ordi-Ros J Labrador M | 2004 | Am J Med2004，116，3： | 1 |
| 12 | Discoidin domain receptor 2 regulates fibroblast proliferation and migration through the extracellular matrix in association with transcriptional activation of matrix metalloproteinase-2显示文摘 | Olaso E Labrador JP Wang L | 2002 | J Biol Chem2002，277，5： | 1 |
| 13 | The start of renal replacement therapy in a Spanish 显示文摘 | Castellano I Gallego S Labrador PJ | 2006 | Nefrologia2006，26，4： | 1 |
| 14 | Dedolomitization in different alkaline media:application to portland cement paste显示文摘 | GARCIA E ALFONSO P LABRADOR M | 2003 | Cement & Concrete Research2003，33，9： | 1 |
| 15 | Discoidin domain receptor 2 regulates fibroblast proliferation and migration through the extracellular matrix in association with transcriptional activation of matrix metalloproteinase-2显示文摘 | Olaso E Labrador JP Wang L | 2002 | Biol Chem2002，277，5： | 1 |
| 16 | Spatial distribution of R&-D expenditure and patent appli-cations across EU regions and its impact on economic co-hesion显示文摘 | GONZALEZ C M GRANADOS C M LABRADOR I S | 2005 | Investigaciones Regionales2005，，6： | 1 |
| 17 | Cold and salt stress regulates the expression and activity of a chickpea cytosolic Cu/Zn superoside dismutase显示文摘 | Josefina Hernandez-Nistal Berta Dopico Emilia Labrador | 2002 | Plant Science2002，163，： | 1 |
| 18 | The prognostic relevance of the nonstructural 5A gene interferon sensitivity determining region is different in infections with genotype 1 b and 3a isolates of hepatitis C virus显示文摘 | Saiz JC Lopez -Labrador FX Ampurdanes S | 1998 | J Infect Dis1998，177，4： | 1 |
| 19 | Topology Maintenance:Extendingthe Lifetime of Wireless Sensor Networks显示文摘 | Wightman P M Labrador M A | 2010 | IEEE Latin AmericaTransactions2010，8，4： | 1 |
| 20 | Interactions of mechanotransduction pathways显示文摘 | LABRADOR V CHEN K D LI Y S | 2003 | Biorheology2003，40，13： | 1 |
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