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| 1 | Genome-wide identification and expression analysis of DNA demethylase family in cotton显示文摘Background:DNA methylation is an important epigenetic factor that maintains and regulates gene expression.The mode and level of DNA methylation depend on the roles of DNA methyltransferase and demethylase,while DNA demethylase plays a key role in the process of DNA demethylation.The results showed that the plant’s DNA demethylase all contained conserved DNA glycosidase domain.This study identified the cotton DNA demethylase gene family and analyzed it using bioinformatics methods to lay the foundation for further study of cotton demethylase gene function.Results:This study used genomic information from diploid Gossypium raimondii JGI(D),Gossypium arboreum L.CRI(A),Gossypium hirsutum L.JGI(AD1) and Gossypium barbadebse L NAU(AD2) to Arabidopsis thaliana.Using DNA demethylase genes sequence of Arabidopsis as reference,25 DNA demethylase genes were identified in cotton by BLAST analysis.There are 4 genes in the genome D,5 genes in the genome A,10 genes in the genome AD1,and 6 genes in the genome AD2.The gene structure and evolution were analyzed by bioinformatics,and the expression patterns of DNA demethylase gene family in Gossypium hirsutum L were analyzed.From the phylogenetic tree analysis,the DNA demethylase gene family of cotton can be divided into four subfamilies:REPRESSOR of SILENCING 1(ROS1),DEMETER(DME),DEMETER-LIKE 2(DML2),and DEMETER-LIKE3(DML3).The sequence similarity of DNA demethylase genes in the same species was higher,and the genetic relationship was also relatively close.Analysis of the gene structure revealed that the DNA demethylase gene family members of the four subfamilies varied greatly.Among them,the number of introns of ROS1 and DME subfamily was larger,and the gene structure was more complex.For the analysis of the conserved domain,it was known that the DNA demethylase family gene member has an endonuclease Ⅲ(END03 c) domain.Conclusion:The genes of the DNA demethylase family are distributed differently in different cotton species,and the gene structure is very different.High expression of ROS1 genes in cotton were under abiotic stress.The expression levels of ROS1 genes were higher during the formation of cotton ovule.The transcription levels of ROS1 family genes were higher during cotton fiber development. | YANG Xiaomin LU Xuke CHEN Xiugui WANG Delong WANG Junjuan WANG Shuai GUO Lixue CHEN Chao WANG Xiaoge WANG Xinlei YE Wuwei | 2019 | Journal of Cotton Research2019，2，3： | 3 |
| 2 | Filter-free self-power CdSe/Sb_(2)(S_(1-x),Se_(x))_(3)nearinfrared narrowband detection and imaging显示文摘Accurate and clear bioimaging is crucial in the field of medical diagnosis.High-quality bioimaging requires to avoid the effects of ambient light as well as the absorption of biological tissues.Nearinfrared(NIR)narrowband detectors located at wavelength from 650 to 900 nm can meet these requirements;thus,they are the potential solution.In this work,we construct a filter-free and self-power NIR narrowband photodetector based on the structure of n-CdSe/p-Sb_(2)(S_(1-x),Se_(x))_(3)heterojunction,and achieve a narrow spectral response at 735 nm with a full width at half-maximum of 35.3 nm in the detector.Further,the imaging characteristics of the NIR narrowband detector are explored,verifying the ability to narrowband detection and imaging.This filter-free and self-power NIR narrowband detector shows considerable promise in real-life applications. | Kanghua Li Yue Lu Xuke Yang Liuchong Fu Jungang He Xuetian Lin Jiajia Zheng Shuaicheng Lu Chao Chen Jiang Tang | 2021 | InfoMat2021，3，10： | 0 |
| 3 | Zinc finger transcription factor ZAT family genes confer multi-tolerances in Gossypium hirsutum L.显示文摘ZAT(Zinc Finger of Arabidopsis thaliana)proteins are composed of a plant-specific transcription factor family,which play an important role in plant growth,development,and stress resista nee.To study the potential function of ZAT family in cotton,the whole genome identification,expression,and structure analysis of ZAT gene family were carried out.In this study,our analysis revealed the presenee of 115Z 56,59,and 115 ZAT genes in Gossypium hirsutum,G raimondii,G.arboreum and G barbadense,respectively.According to the number of domains and phylogenetic characteristics,we divided ZAT genes of four Gossypium species into 4 different clades,and further divided them into 11 subfamilies.The results of collinearity an alysis showed that segmental duplicati on was the main method to amplify the cotton ZAT genes family.Analysis of c/s-elements of promoters indicated that most GhZAT genes contained c/5-elements related to plant hormones and abiotic stress.According to heatmap analysis,the expression patterns of GhZAT genes under different stresses indicated that GhZAT genes were significantly involved in the response to cold,heat,salt,and PEG stress,possibly through different mechanisms.Among the highly expressed genes,we cloned a G hirsutum gene GhZAT67.Through virus-induced gene silencing(VIGS),we found that its expression level decreased significantly after being sileneed.Under alkaline treatment,the wilting degree of silenced plants was even greater than the wild type,which proved that GhZAT67 gene was involved in the response to alkaline stress. | FAN Yapeng ZHANG Yuexin RUI Cun XU Nan ZHANG Hong WANG Jing MALIK Waqar Afeal HAN Mingge ZHAO Lanjie LU Xuke CHEN Xiugui CHEN Chao YE Wuwei | 2021 | Journal of Cotton Research2021，4，3： | 0 |
| 4 | Rapid thermal evaporation for cadmium selenide thin-film solar cells显示文摘Cadmium selenide(CdSe)belongs to the binary II-VI group semiconductor with a direct bandgap of~1.7 eV.The suitable bandgap,high stability,and low manufacturing cost make CdSe an extraordinary candidate as the top cell material in silicon-based tandem solar cells.However,only a few studies have focused on CdSe thin-film solar cells in the past decades.With the advantages of a high deposition rate(~2µm/min)and high uniformity,rapid thermal evaporation(RTE)was used to maximize the use efficiency of CdSe source material.A stable and pure hexagonal phase CdSe thin film with a large grain size was achieved.The CdSe film demonstrated a 1.72 eV bandgap,narrow photoluminescence peak,and fast photoresponse.With the optimal device structure and film thickness,we finally achieved a preliminary efficiency of 1.88%for CdSe thin-film solar cells,suggesting the applicability of CdSe thin-film solar cells. | Kanghua LI Xuetian LIN Boxiang SONG Rokas KONDROTAS Chong WANG Yue LU Xuke YANG Chao CHEN Jiang TANG | 2021 | Frontiers of Optoelectronics2021，14，4： | 0 |
| 5 | Characters and structures of the nucleobase-ascorbate transporters(NAT)family genes in Gossypium hirsutum and their roles in responding to salt and drought stresses显示文摘Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant di erent between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was conducted for the first time in cotton.Results: In this study, we discovered 32, 32, 18, and 16 NAT genes in Gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups(groups I–IV) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT members in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under di erent conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resistance to salt and drought stress through the virus-induced gene silencing(VIGS) analysis.Conclusions: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analysis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to biotic and abiotic resistance for the improvement in cotton. | GUO Lixue ZHAO Lanjie LU Xuke CHEN Xiugui WANG Shuai WANG Junjuan WANG Delong YIN Zujun CHEN Chao FAN Yapeng ZHANG Yuexin YE Wuwei | 2022 | Journal of Cotton Research2022，5，2： | 0 |
| 6 | Functional structure analysis and genome‑wide identification of CNX gene family in cotton显示文摘Background:Under abiotic stress conditions,cotton growth is inhibited and yield losses are severe.Identification of calnexin family members and function analysis under abiotic stress laid the foundation for the screening of stressrelated candidate genes.Results:A total of 60 CNX family members have been identified in Gossypium hirsutum,G.barbadense,G.arboreum,and G.raimondii,and they were divided into two categories:CNX and CRT genes.Through the construction of a phylogenetic tree,they were subdivided into three classes.Further analysis of chromosome localization,conserved promoters,gene structure and selection under pressure showed that the family members were highly conserved in the evolution process.Analysis of cis-acting elements in the promoter regions showed that CNX family genes contain regulatory elements for growth and development,anaerobic,drought,defense and stress response,and plant hormones.Using RNA-seq data to study the expression pattern of GhCNX genes under cold,hot,salt stress and Polyethylene glycol,it was observed that the gene expression levels changed by different degrees under different stress conditions,indicating that GhCNX members were involved in the regulation of multiple biological stresses.Conclusion:This study provides an insight into the members of cotton CNX genes.The results of this study suggested that CNX family members play a role in defense against adversity and provide a foundation for the discovery of stress-related genes. | XU Nan ZHANG Hong ZHANG Yuexin FAN Yapeng WANG Jing MALIK Waqar Afzal RUI Cun HAN Mingge LU Xuke CHEN Xiugui WANG Junjuan WANG Delong WANG Shuai CHEN Chao GUO Lixue ZHAO Lanjie YE Wuwei | 2022 | Journal of Cotton Research2022，5，3： | 0 |
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