|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | First observation of Karlodinium venef icum from the East China Sea and the coastal waters of Germany显示文摘Harmful algal blooms often occurred in the East China Sea (ECS) and the German coastal waters of the North Sea but Karlodinium veneficum had not been taxonomically reported. Two strains of Karlodinium (LAMB090611 and LAMB010601) were isolated from the two areas in 2009. The morphological characteristics and molecular phylogeny of two strains are compared on the basis of observation of a light microscope, a scanning electron microscope (SEM), a laser scanning micro-scope (LSM) and an internal transcribed spacer (ITS) sequence data. The mean cell length of strain LAMB090611 is (14.2 ± 1.8) μm (range 11.1-18.7 μm) and mean width is (10.8 ± 1.5) μm (range 8.2- 14.7 μm). The mean cell length of strain LAMB010601 is (15.1 ± 1.2) μm (range 12.7-17.9 μm) and the mean width is (11.4 ± 1.1) μm (range 9.1-14.7 μm), respectively. The two strains are similar in morphological characteristics, including a straight apical groove, distinct ventral pore, sulcal extension, cingulum displacement, two or four irregular shaped chloroplasts within the cell and almost equal sized epicone and hypocone. The large and round nucleus is located at the center or at the hypocone of the cell. The sequence length of the ECS strain LAMB090611 and the German strain LAMB010601 is 640 and 646 bp, respectively. The GC content is 49%. The nucleotide similarity of the two strains is 98.1%. The sequence divergence is 0.003. Both strains are confirmed as Karlo-dinium veneficum (D. Ballantine) J. Larsen and this is the first taxonomic report from China and Germany coastal waters. The population dynamics of this toxic species in the ECS and German coastal waters needs to be investigated in the near future. | WANG Hongxia LU Douding HUANG Haiyan GOBEL Jeaaette DAI Xinfeng XIA Ping | 2011 | Acta Oceanologica Sinica2011，30，6： | 7 |
| 2 | Biomarker records in penguin droppings and observed changes in penguin communities and their response to the ENSO in the Western Antarctic显示文摘Lipid biomarkers in AD2 penguin droppings-amend soil core from the Ardley Island,Western Antarctic,were dated using 210Pb.Changes in the fatty acid ratios of nC18:2/nC18:0 from the penguin droppings reflect climate changes coincident with ENNO events during 1931-2006.The occurrence of the minimum values in the depth of 2-3 and 6-7 cm are consistent with the end of ENSO in 1958 and 1983,respectively,reflecting a lag of the biomarker records in AD2 penguin droppings-amend soil in climatic signatures.This study also reveals that the changes in the relative concentration of n-alkanes nC23,the ratios of nC23/nC17 and nC21 /nC22+,and carbon preferential index(CPI) values collectively indicate the variations of soil microor-ganism and lower plant,which are closely related to climate changes.The ratios of bacterial fatty acids iC15:0/aC15:0 reflect the increasing significance of microorganism activities during the two periods that occurred at the end years of ENSO.Decrease in CPIA value and increase in nC21 /nC22+ indicate that low molecular weight fatty acids are derived from microorganism;and their insignificant correlation with Pr/Ph suggests microorganisms play an important role in the relatively simply ecosystem in the Antarctic and are closely linked to climatic conditions.In addition,the observed penguin community indicates the popula-tion of penguin can largely reflect the impacts of global climate changes on the ecosystem. | ZHANG HaiSheng LU DouDing YU PeiSong ZHANG WeiGuo LU Bing Hans-Ulrich PETER Walter VETTER | 2012 | Science China Earth Sciences2012，55，8： | 6 |
| 3 | Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Karenia mikimotoi by fluorescence in situ hybridization显示文摘Harmful algal blooms recently have been under the spotlight throughout the world, because of their negative impact on the marine environment, aquaculture, fisheries as well as public health. The development of methods for rapid and precise identification and quantification of causative species is essential for the warning and monitoring of blooms, among which the techniques based on taxonomic probes are the most favored. In this study, two harmful algae, i.e., Prorocentrum minimum and Karenia mikimotoi were taken into consideration. The partial large subunit rDNA (D1-D2) of both species were firstly PCR-amplified, cloned and sequenced. The obtained sequences were then introduced to carry out alignment analysis for gene specific regions. Three respective candidate probes for each species were designed and used to screen the optimal probe by performing fluorescence in situ hybridization (FISH) tests. The results showed that the probes Pmin0443 and Kmik0602 displayed the best hybridization for P. minimum and K. mikimotoi, respectively. Both the specific (taxonomic) (Pmin0443 and Kmik0602) and the control probes (UniC0512 and UniR0499) were used for cross-reactivity tests with other microalgae in our laboratory. The probes Pmin0443 and Kmik0602 are specific and could be served as taxonomic probes introduced into the techniques targeting rRNA, such as FISH, sandwich hybridization, and DNA-microarray assay of P. minimum and K. mikimotoi in the future. Finally, FISH analyses with both probes were performed on the simulated field samples. The probes could hybridize exclusively with the target cells well, and no significant difference (p >0.05) was observed in the cell densities of the samples determined by FISH and light microscopy (LM). All suggest that the probes are specific and could be introduced into FISH for the monitoring of both harmful algae. | CHEN Guofu WANG Quanfu ZHANG Chunyun ZHANG Baoyu WANG Guangce LU Douding XU Zhong YAN Peishen | 2013 | Acta Oceanologica Sinica2013，32，2： | 1 |
| 4 | Morphological and genetic study of Prorocentrum donghaiense Lu from the East China Sea, and comparison with some related Prorocentrum species 显示文摘 | Douding Lu | 2005 | Harmful Algae2005，4，： | 1 |
| 5 | Morphological and genetic study of Prorocentrum donghaiense Lu from the East China Sea, and comparison with some related Prorocentrum species显示文摘 | Douding Lu | 2005 | Harmful Algae2005，4，： | 1 |
| 6 | Distribution of indicator dinoflagellates in the East China Sea显示文摘-The material discussed in this paper is from four cruises which were carried out in May -June, 1986 (spring), July -August, 1987(summer) .October -November, 1988(autumn)and December, 1987(winter)respectively. Identified dinoflagellates were 141 species (including varieties and forms) belonging to 18 genera.According to the distribution and ecological characteristics of dinoflagellates species, we can divide them into three groups: 1) Hyporhaline-neritic group: they are distributed only in type Ⅲ and typeⅣ water and they perform an evident function indicating continental coast water; 2) Eurythermic-euryhaline group represented by Protoperidinium depressum, Gentium fusus, etc. They are fond of perching in mixing water which character is similar to that of coast water even though they are distributed in different water systems; 3) Hyperthermic-hyperhaline group: on the basis of varying degrees to which they tolerate temperature and salinity, we can divide them into two sub-groups: | Lu Douding Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China | 1992 | Acta Oceanologica Sinica1992，11，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员