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| 1 | Early dynamic transcriptomic changes during preoperative radiotherapy in patients with rectal cancer: A feasibility study显示文摘AIM: To develop novel biomarkers of rectal radiotherapy, we measured gene expression profiles on biopsies taken before and during preoperative radiotherapy. METHODS: Six patients presenting with a locally advanced rectal cancer (T>T2, N0/Nx, M0) eligible for preoperative radiotherapy (45 Gy in 25 fractions) were selected in a pilot study. Six tumor and 3 normal tissues biopsies were taken before and during radiotherapy,after a dose of 7.2 Gy at a median time of 1 h following irradiation (0:27-2:12). Tumor or normal tissue purity was assessed by a pathologist prior to RNA extraction. Mean RNA content was 23 μg/biopsy (14-37) before radiotherapy and 22.7 μg/biopsy (12-35) during radiotherapy. After RNA amplification, biopsies were analysed with 54K HG-U133A Plus 2.0 Affymetrix expression micro-arrays. Data were normalized according to MAS5 algorithm. A gene expression ratio was calculated as: (gene expression during radiotherapy-gene expression before radiotherapy)/gene expression before radiotherapy. Were selected genes that showed a ratio higher than ± 0.5 in all 6 patients. RESULTS: Microarray analysis showed that preoperative radiotherapy significantly up-regulated 31 genes and down-regulated 6 genes. According to the Gene Ontology project classification, these genes are involved in protein metabolism (ADAMDEC1 ; AKAP7 ; CAPN5 ; CLIC5 ; CPE ; CREB3L1 ; NEDD4L ; RAB27A), ion transport (AKAP7 ; ATP2A3 ; CCL28 ; CLIC5 ; F2RL2 ; NEDD4L ; SLC6A8), transcription (AKAP7 ; CREB3L1 ; ISX ; PAB-PC1L ; TXNIP), signal transduction (CAPN5 ; F2RL2 ; RA- B27A ; TNFRSF11A), cell adhesion (ADAMDEC1 ; PXDN ; SPON1 ; S100A2), immune response (CCL28 ; PXDN ; TNFRSF11A) and apoptosis (ITM2C ; PDCD4 ; PVT1). Up-regulation of 3 genes (CCL28 ; CLIC5 ; PDCD4) was detected by 2 different probes and up-regulation of 2 genes (RAB27A ; TXNIP) by 3 probes. CONCLUSION: Micro-arrays can efficiently assess early transcriptomic changes during preoperative radiotherapy for rectal cancer, and may help better understand tumor radioresistance. | Stephane Supiot Wilfried Gouraud Loc Campion Pascal Jezéquel Bruno Buecher Josiane Charrier Marie-Francoise Heymann Marc-Andre Mahé Emmanuel Rio Michel Chérel | 2013 | World Journal of Gastroenterology2013，19，21： | 2 |
| 2 | Randomized trial on fluorides and sealants for fissure caries prevention显示文摘 | Liu BY Lo EC Chu CH | 2012 | J Dent Res2012，91，8： | 1 |
| 3 | Emotional management and 5-HT2Areceptor gene variance in patients with schizophrenia显示文摘 | Lo CH Tsai GE Liao CH | 2010 | Biol Psychol2010，83，2： | 1 |
| 4 | Arthroscopy-assisted surgery for tibial plateau fractures:2-to 10-year follow-up results显示文摘 | Chan YS Chiu CH Lo YP | 2008 | Arthroscopy2008，24，7： | 1 |
| 5 | White spot syndrome baculovirus (WSSV) detected in cultured and captured shrimp,crabs and other arthropods 显示文摘 | Ho CH Peng SE | 1996 | Dis Aquat Org1996，27，： | 1 |
| 6 | Caries status of preschool children in Hong Kong显示文摘 | Chu CH Fung DSH Lo ECM | 1999 | Br Dent J1999，187，： | 1 |
| 7 | A novel surgical technique: single-incision transum- bilical laparoscopic Roux-en-Y gastric bypass 显示文摘 | Huang CK Yao SF Lo CH | 2010 | Obes Surg2010，20，10： | 1 |
| 8 | Risk factors and management of intraabdominal infection after extended radical gastrectomy显示文摘 | Lo CH Chen JH Wu CW | 2009 | Am J Surg2009，，6： | 1 |
| 9 | Effects of group music intervention on psychiatric symptoms and depression in patient with schizophrenia显示文摘 | Lu SF Lo CH Sung HC | 2013 | Complement Ther Med2013，，06： | 1 |
| 10 | Arthroscopy - assisited surgery for tibialplateau fractures :2 -to 10 -yer follow- up results 显示文摘 | Chan YS Chiu CH Lo YP etal | 2008 | Arthroscopy2008，24，7： | 1 |
| 11 | Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction显示文摘 | Lo CF Leu JH Ho CH | 1996 | Diseases of Aquatic Organisms1996，25，12： | 1 |
| 12 | Growth hormone or insulin￣like growth factor I increases fat oxidation and decreases protein oxidation without altering energy expenditure in parenterally fed rats显示文摘 | Lo CH Hirvomen MD Kritsch KR | 1997 | Am J Clin nutr1997，65，： | 1 |
| 13 | Assay of enoxacin in human and prostatic tissue by HPLC 显示文摘 | White LO Bowyer HM McMullin CH | 1988 | J Antimicrob Chemother1988，21，： | 1 |
| 14 | Adherence behaviours in Taiwancse children and adolescents with type I diabetes mellitus 显示文摘 | Chang CW Yeh CH Lo FS | 2007 | Journal of Clinical Nursing2007，16，7： | 1 |
| 15 | Uncaria rhynehophylla upregulates the expression of MIF and cyclophilin A in kainic acid-induced epi- lepsy rats : A proteomie analysis 显示文摘 | Lo WY Tsai FJ Liu CH | 2010 | Am J Chin Med2010，38，4： | 1 |
| 16 | 3-D Moment Forms:Their Construction and Application to Object Identification and Positioning显示文摘 | LO CH DON HS | 1989 | IEEE Transactions on pattern analysis and machine intelligence1989，11，10： | 1 |
| 17 | l)etermination of volatile organic compounds in workplace air by multlsorbent adsorption/thermal de- sorption- GC/MS显示文摘 | Wu CH Feng CT Lo YS | 2004 | Chemosphere2004，56，： | 1 |
| 18 | Synthesis and characteri- zation of positive-charge functionalized mesoporous silica nanoparticles for oral drug delivery of an anti-inflammato- ry drug显示文摘 | Lee CH Lo LW Mou CY | 2008 | Adv Funct Mater2008，18，20： | 1 |
| 19 | Antitumor and antimetastatic activity of IL-23显示文摘 | Lo CH Lee SC Wu PY | 2003 | J Immunol2003，171，2： | 1 |
| 20 | Arthroscopy-assisted surgery for tibial plateau fractures:2-to 10-year follow-up results显示文摘 | Chan YS Chiu CH Lo YP | 2008 | Arthroscopy2008，24，7： | 1 |
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