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| 1 | Preparation of Anti-cardiac Troponin I Monoclonal Antibodies and Their Characterization with Surface Plasmon Resonance Biosensor显示文摘Cardiac troponin I(cTnI) was separated and purified from human left ventricular tissue by affinity chromatographic method and used to immunize Balb/c mice by intraperitoneal injection and four hybridoma cell lines, which secreted monoclonal antibody(mAb) against human cTnI, were obtained by cell fusion, identification and cloning twice. Three mAbs(9F5, 2F11, 8C12) were produced from the ascites of Balb/c mice injected intraperitoneally the hybridoma cells and characterized by means of a surface plasmon resonance(SPR) biosensor. An optimal and specific sensing membrane for troponin I was prepared with staphylococcal protein A(SPA) as the intermediate layer and mAb against human cTnI as the capture antibody. On the basis of the sensing membrane, two modes of operation of the SPR biosensor were developed, i.e ., a direct detection of antigen antibody affinity and a sandwich assay. In the sandwich assay detection mode, the mAbs competition was measured by monitoring whether the secondary antibody had been attached to the cTnI already captured by the first antibody on the sensor surface. The SPR biosensor was shown to be able to directly detect the antigen antibody affinity and the order of the affinity was found to be 9F5>2F11>8C12. In the sandwich detection mode, it was found that the different epitopes on the cTnI molecules were recognized by the three mAbs respectively, but the asymmetrical competition was shown between 2F11 and 8C12 and no competition was found between 9F5 and 2F11 or 8c12. Based on these results, a double monoclonal sandwich immunoassay for cTnI was developed by using the optimal antibody pair of 9F5 and 2F11 and the SPR biosensor with SPA substrate membrane, which showed an excellent sensitivity of 0.8 μg/L for both the buffer and the serum samples compared with the direct detection of cTnI for the buffer with the lowest detection limit of 4 μg/L and conventional ELISA with the sensitivity of 1.9 μg/L. | WEIJing-yan LIUXia SONGDa-qian BULi-sha HUXing MUYing | 2003 | Chemical Research in Chinese Universities2003，19，2： | 4 |
| 2 | Activities of the Enzymes Involved in Starch Synthesis and Starch Accumulation in the Grains of Wheat Cultivars, GC8901 and SN1391显示文摘Two wheat cultivars, GC8901 (hard winter wheat) and SN1391 (soft winter wheat), were used for investigating the changes of enzyme activities for sucrose metabolism and starch biosynthesis and the accumulation character of starch composition. The result showed that activities of sucrose (SS), sucrose-phosphate synthase (SPS), adenosine diphosphorate glucose pyrophrylase (AGPase) and soluble starch syntheses (SSS) of 1391, which have more starch, were significant higher than those of 8901, that with low starch content. But the changing of granule-bound starch synthase (GBSS) activity was consistent with the amylose content, which indicated that amylose contents in grain were determined by GBSS activity, especially the activity at later grain filling stages. Simulating with Richards equation showed that it was initiating time and accumulation rate, but not accumulation duration that determined the content of starch composition. Furthermore, changing of sucrose transport capacity was consistent with SSS and GBSS activities, starch accumulation rate was accordant to AGPase and SS/SPS ration, not SS, SPS, SSS or GBSS activities. The results suggested that there was no inevitable relation of starch accumulating rate and starch composition contents with the activity of single enzyme such as SS, SPS, SSS or GBSS, but closely related to AGPase activity and SS/SPS ratio, and it was SPS and AGPase that play a vital role in the biosynthetic pathway. Later polymerization reactions catalyzed by SSS and GBSS don’t seem to control the rate of starch accumulation, but do affect starch structure. | LIUXia JIANGChun-ming ZHENGZe-rong ZHOUZhu-nan HEMing-rong WANGZhen-lin | 2005 | Agricultural Sciences in China2005，4，5： | 2 |
| 3 | Agrobacterium-mediated Transformation of Rice (Oryza sativa L.) with Atrazine Chlorohydrolase Gene (atzA)显示文摘Atrazine chlorohydrolase gene (atzA) was cloned from Arthrobacter sp. AD1. A plant expression plasmid was constructed under the control of CaMV35s promoter and was used in rice transformation. The target gene was successfully introduced into mature embryos of a japonica rice cultivar Jindao 107 by Agrobacterium- mediated transformation and hundreds of transgenic plants were obtained. The exogenous atzA gene in the transgenic plants that expressed atrazine resistance was confirmed by Southern blot hybridization. The resistance experiments by spraying transgenic rice plants with 0.133% atrazine shown that most of the transgenic rice plants exhibited the resistance to herbicide atrazine. The segregation of exogenous atzA gene in T1 progeny corresponded to the Mendelian ratio. | WANGSong-wen SHILi-li SUNZong-xiu CAIBao-li FUYa-ping WANGYang SIHua-min LIUXia ZHANGXin | 2005 | Agricultural Sciences in China2005，4，4： | 2 |
| 4 | YAG:Ce nano-sized phosphor particles prepared by a solvothermal method显示文摘 | LIUXIA LIU HONG WANG JIYANG | 2004 | Materials Research Bulletin2004，39，： | 1 |
| 5 | Alternatefurrow irrigation and nitrogen level effects on migriation of waterand nitrate-nitrogen in soil and root growth of cucumber in solar-greenhouse显示文摘 | Lidong Zhang Lihong Gao Liuxia Zhang | 2012 | Scientia Horticulturae2012，138，： | 1 |
| 6 | 显示文摘 | LIUXia(刘霞) LILei(李蕾) LIWen-jin(李文进) YANGYang(杨阳) MAOLu-gang(毛禄刚) | 2013 | 食品安全质量检测学报2013，2，： | 1 |
| 7 | N-methylimi-dazolium anion-exchangestationaryphaseforhigh-per-formanceliquidchromatography显示文摘 | QiuHongdeng JiangShengxiang LiuXia | 2006 | JournalofChroma-tographyA2006，1103，2： | 1 |
| 8 | Detection of autoantibodies to multiple tumor-associated antigens inthe immunodiagnosis of ovarian cancer显示文摘 | Liuxia Li Kaijuan Wang Liping Dai Peng Wang Xuan-Xian Peng Jian-Ying Zhang | 2008 | Molecular Medicine Reports2008，，4： | 1 |
| 9 | Evaluating Behavioral Risk Factor Interventions for Hypertensive and Diabetic Patient Management in the National Basic Public Health Service Programs from 2009显示文摘BACKGROUND In recent decades,China has faced a double burden of infectious diseases and noncommunicable diseases(NCDs).The number of deaths due to NCDs accounts for 88.5%of total deaths in China in 2019.To address these public health issues and improve the accessibility and affordability of primary health care,a National Basic Public Health Service Program(NBPHSP)was introduced as part of China’s healthcare reform initiated in 2009.Subsided by national and local government budgets,NBPHSP is mainly implemented by community and township health service centers,as well as health stations in urban areas and village clinics in rural areas(1).Health management of patients with hypertension and diabetes is an integral and pivotal part of the NBPHSP. | Liuxia Yan Ning Ji Jian Xu Min Liu Lizheng Guan Kejun Liu Ainan Jia Xianbin Ding Dan Liu Man Li Yanfang Zhao Shengquan Mi Wenhua Zhao Zhuoqun Wang Yamin Bai | 2022 | China CDC weekly2022，4，19： | 0 |
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