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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Epidemiologic characteristics of Helicobacter pylori infection in southeast Hungary显示文摘BACKGROUND Epidemiologic studies have revealed a decrease in the prevalence of Helicobacter pylori(H.pylori)infection in Western Europe.AIM To obtain data regarding the prevalence of H.pylori in Csongrád and Békés Counties in Hungary,evaluate the differences in its prevalence between urban and rural areas,and establish factors associated with positive seroprevalence.METHODS One-thousand and one healthy blood donors[male/female:501/500,mean age:40(19–65)years]were enrolled in this study.Subjects were tested for H.pylori IgG antibody positivity via enzyme-linked immunosorbent assay.Subgroup analysis by age,gender,smoking habits,alcohol consumption,and urban vs nonurban residence was also performed.RESULTS The overall seropositivity of H.pylori was 32%.It was higher in males(34.93%vs 29.2%,P=0.0521)and in rural areas(36.2%vs 27.94%,P=0.0051).Agricultural/industrial workers were more likely to be positive for infection than office workers(38.35%vs 30.11%,P=0.0095)and rural subjects in Békés County than those in Csongrád County(43.36%vs 33.33%,P=0.0015).CONCLUSION Although the prevalence of H.pylori infection decreased in recent decades in Southeast Hungary,it remains high in middle-aged rural populations.Generally accepted risk factors for H.pylori positivity appeared to be valid for the studied population. | Lenke Bálint Andrea Tiszai Gábor Kozák Ilona Dóczi Veronika Szekeres Orsolya Inczefi Georgina Ollé Krisztina Helle Richárd Róka András Rosztóczy | 2019 | World Journal of Gastroenterology2019，25，42： | 5 |
| 3 | Frequency and prognostic role of mucosal healing in patients with Crohn's disease and ulcerative colitis after one-year of biological therapy显示文摘AIM:To assess the endoscopic activity before and after a one-year period of biological therapy and to evaluate the frequency of relapses and need for retreatment after stopping the biologicals in patients with Crohn’s disease(CD)and ulcerative colitis(UC).METHODS:The data from 41 patients with CD and 22 patients with UC were assessed.Twenty-four CD patients received infliximab,and 17 received adalimumab.The endoscopic severity of CD was quantified with the simplified endoscopic activity score for Crohn’s disease in CD and with the Mayo endoscopic subscore in UC.RESULTS:Mucosal healing was achieved in 23 CD and7 UC patients.Biological therapy had to be restarted in78%of patients achieving complete mucosal healing with CD and in 100%of patients with UC.Neither clinical remission nor mucosal healing was associated with the time to restarting the biological therapy in either CD or UC.CONCLUSION:Mucosal healing did not predict sustained clinical remission in patients in whom the biological therapies had been stopped. | Klaudia Farkas Péter László Lakatos Mónika Szcs va Pallagi-Kunstár Anita Bálint Ferenc Nagy Zoltán Szepes Noémi Vass Lajos S Kiss Tibor Wittmann Tamás Molnár | 2014 | World Journal of Gastroenterology2014，20，11： | 2 |
| 4 | Free vibration analysis of a uniform cantilever beam with point masses by an analytical-and-numerical-combined method 显示文摘 | WU J S LINT L | 1990 | Journal of Sound and Vibration Mechanics1990，，136： | 1 |
| 5 | A Robust and Efficient Method for Fusing Heterogeneous Data from Traffic Sensors on Freeways 显示文摘 | VAN LINT J W C HOOGENDOORN S P | 2010 | Computer-Aided Civil and Infrastructure Engineering2010，25，8： | 1 |
| 6 | Biomarker evidence for widespread anaerobic methane oxidation in Mediterranean sediments by a consortium of methanogenic archaea and bacteria显示文摘 | Pancost R D Sinninghe Damste J S De Lint S | 2000 | Appl Environ Microbiol2000，66，： | 1 |
| 7 | Combination of video - assisted thoraeoseop ic surgery and hand - assisted laparoscopic surgery for early cancer of the thoracic esophagus显示文摘 | LINT S KUO S J LAI C Y | 2004 | Int Surg2004，89，3： | 1 |
| 8 | Effect of combining dexmedetomidine and morphine for intravenous patient- controlled analgesia显示文摘 | LINT F YEH Y C LIN F S | 2009 | Br J Anaesth2009，102，1： | 1 |
| 9 | Metabolism of tramadol iu man and ani- mal显示文摘 | Lint LV Erlau S Fraukus LH | 1981 | Aiznein Forsch1981，31，2： | 1 |
| 10 | Tsunami-like solitary waves impinging and overtopping an impermeable seawall: experiment and RANS mod- eling显示文摘 | HSIAO S C LINT C | 2010 | Coastal Engineering2010，57，1： | 1 |
| 11 | Mechanism of activation of protein kinase D2(PKD2) by the CCK(B)/gastrin receptor显示文摘 | Sturany S Van Lint J Gilchrist A | 2002 | J Biol Chem2002，277，29： | 1 |
| 12 | Seeing diabetes visual detection of glucose based on the intrinsic peroxidase - like activity of MoS2 nanosheets显示文摘 | LINT R ZHONG L S GUO L Q | 2014 | Nanoscale2014，6，11： | 1 |
| 13 | Analysis of risk factors-especially different types of plexitis-for postoperative relapse in Crohn's disease显示文摘AIM To evaluate the presence of submucosal and myenteric plexitis and its role in predicting postoperative recurrence.METHODS Data from all patients who underwent Crohn's disease(CD)-related resection at the University of Szeged, Hungary between 2004 and 2014 were analyzed retrospectively. Demographic data, smoking habits, previous resection, treatment before and after surgery, resection margins, neural fiber hyperplasia, submucosal and myenteric plexitis were evaluated as possible predictors of postoperative recurrence. Histological samples were analyzed blinded to the postoperative outcome and the clinical history of the patient. Plexitis was evaluated based on the appearance of the most severely inflamed ganglion or nerve bundle. Patients underwent regular follow-up with colonoscopy after surgery. Postoperativerecurrence was defined on the basis of endoscopic and clinical findings, and/or the need for additional surgical resection. RESULTS One hundred and four patients were enrolled in the study. Ileocecal, colonic, and small bowel resection were performed in 73.1%, 22.1% and 4.8% of the cases, respectively. Mean disease duration at the time of surgery was 6.25 years. Twenty-six patients underwent previous CD-related surgery. Forty-three point two percent of the patients were on 5-aminosalicylate, 20% on corticosteroid, 68.3% on immunomodulant, and 4% on anti-tumor necrosis factor-alpha postoperative treatment. Postoperative recurrence occurred in 61.5% of the patients; of them 39.1% had surgical recurrence. 92.2% of the recurrences developed within the first five years after the index surgery. Mean disease duration for endoscopic relapse was 2.19 years. The severity of submucosal plexitis was a predictor of the need for second surgery(OR = 1.267, 95%CI: 1.000-1.606, P = 0.050). Female gender(OR = 2.21, 95%CI: 0.98-5.00, P = 0.056), stricturing disease behavior(OR = 3.584, 95%CI: 1.344-9.559, P = 0.011), and isolated ileal localization(OR = 2.671, 95%CI: 1.033-6.910, P = 0.043) were also predictors of postoperative recurrence. No association was revealed between postoperative recurrence and smoking status, postoperative prophylactic treatment and the presence of myenteric plexitis and relapse.CONCLUSION The presence of severe submucosal plexitis with lymphocytes in the proximal resection margin is more likely to result in postoperative relapse in CD. | Agnes Milassin Anita Sejben László Tiszlavicz Zita Reisz Gyorgy Lázár Mónika Szucs Renáta Bor Anita Bálint Mariann Rutka Zoltán Szepes Ferenc Nagy Klaudia Farkas Tamás Molnár | 2017 | World Journal of Gastrointestinal Surgery2017，9，7： | 1 |
| 14 | Group Decision Making Using Incomplete Fuzzy Preference Relations Based on the Additive Consistency and the Order Consistency 显示文摘 | CI-IEN S M LINT E LEE L W | 2014 | In-formationSciences2014，259，3： | 1 |
| 15 | Purification and characterization of PE from Ficus awkeotsang Makio achenes显示文摘 | LINT P LIU C C CHEN S W | 1989 | Plant Physiology1989，91，14： | 1 |
| 16 | Application of the charge regulation model to transport of ions through hydrophilic membranes: one-dimensional transport model for narrow pores (nanofiltration)显示文摘 | de Lint W B S Biesheuvel P M Verweij H | 2002 | Journal of Colloid and Interface Science2002，251，： | 1 |
| 17 | A Piezoelectric Micropump Based on Micromachining of Silicon显示文摘 | H van Linte F van de Pol S Bouwstra | 1988 | Sensors and Actuators A1988，15，： | 1 |
| 18 | Coherent interference suppression with complementally transformed adaptive beamformer显示文摘 | LEE T S LINT T | 1998 | IEEE Transactions on Antennas and Propagation1998，46，5： | 1 |
| 19 | Accurate freeway travel time prediction with state-space neural networks under missing data显示文摘 | Van Lint J Hoogendoorn S Van Zuylen H | 2005 | Transportation Research Part C2005，13，： | 1 |
| 20 | Association of a T29-C polymorphism of the transforming growth factor-beta 1 gene with genetic Susceptibility to myocardial infarction in Japanese 显示文摘 | YOKOTA M ICHIHARA S LINT L | 2000 | Circulation2000，101，24： | 1 |
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